EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Juan C. ALARCÓN P., Hader I. CASTAÑO P., Ligia L. CORRALES G., Silvia L. JIMÉNEZ R., Abel DÍAZ C.
Formato: article
Lenguaje:EN
Publicado: Universidad de Antioquia 2009
Materias:
Acceso en línea:https://doaj.org/article/5c12fc5844914b3aba719b9f52506988
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:5c12fc5844914b3aba719b9f52506988
record_format dspace
institution DOAJ
collection DOAJ
language EN
topic calli
Bixa orellana
annatto.
Food processing and manufacture
TP368-456
Pharmaceutical industry
HD9665-9675
spellingShingle calli
Bixa orellana
annatto.
Food processing and manufacture
TP368-456
Pharmaceutical industry
HD9665-9675
Juan C. ALARCÓN P.
Hader I. CASTAÑO P.
Ligia L. CORRALES G.
Silvia L. JIMÉNEZ R.
Abel DÍAZ C.
EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES
description <!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} @font-face {font-family:Aldine401BT-RomanA; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"MS Mincho"; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 134676480 16 0 131073 0;} @font-face {font-family:Verdana; panose-1:2 11 6 4 3 5 4 4 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:536871559 0 0 0 415 0;} @font-face {font-family:"\@Aldine401BT-RomanA"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:0 134676480 16 0 131072 0;} @font-face {font-family:TimesNewRomanPS-ItalicMT; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:0; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} @font-face {font-family:SymbolMT; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"MS Mincho"; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:0 134676480 16 0 131072 0;} @font-face {font-family:"\@SymbolMT"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:0 134676480 16 0 131072 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Recent evidences suggest annatto can be constituted in a therapeutic alternative by its direct and indirect influences against the poison of Bothrops asper, cause of most of snake bite in our country. The ex- vitro metabolic production of this plant is not obtained in early growth stages but it is possible to obtain it by means of cell cultures. This research evaluates some inductive calli combinations of plant segments of Bixa orellana L, like leaves, roots and stems grown in vitro, in order to get high induction rates and bigger amounts of tissue precursors of cellular suspensions, potentially producers of active metabolites. These explants, located in nutritious means without growth regulators, or with supplements of some combinations of them, are stored for eight weeks (25} 1º C and darkness) and the calli formation percentage tested in second, fifth and eighth week of growing. The calli generation in explants from roots is low, whereas in those from stems and leaves is favored with the treatment and with exposition time to the growth regulators. Regardless of the kind of explant used, the calli formation percentage is increased by the treatment that uses NAA (5 _M) and kinetine (4.64 _M), and showing values of 17,0 %, 58,9 % and 77,5 % for roots, stems and leaves respectively.
format article
author Juan C. ALARCÓN P.
Hader I. CASTAÑO P.
Ligia L. CORRALES G.
Silvia L. JIMÉNEZ R.
Abel DÍAZ C.
author_facet Juan C. ALARCÓN P.
Hader I. CASTAÑO P.
Ligia L. CORRALES G.
Silvia L. JIMÉNEZ R.
Abel DÍAZ C.
author_sort Juan C. ALARCÓN P.
title EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES
title_short EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES
title_full EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES
title_fullStr EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES
title_full_unstemmed EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES
title_sort evaluation of some growth regulators combination for callus induction in annatto (<i>bixa orellana</i> l.) active plant against snake bites
publisher Universidad de Antioquia
publishDate 2009
url https://doaj.org/article/5c12fc5844914b3aba719b9f52506988
work_keys_str_mv AT juancalarconp evaluationofsomegrowthregulatorscombinationforcallusinductioninannattoibixaorellanailactiveplantagainstsnakebites
AT hadericastanop evaluationofsomegrowthregulatorscombinationforcallusinductioninannattoibixaorellanailactiveplantagainstsnakebites
AT ligialcorralesg evaluationofsomegrowthregulatorscombinationforcallusinductioninannattoibixaorellanailactiveplantagainstsnakebites
AT silvialjimenezr evaluationofsomegrowthregulatorscombinationforcallusinductioninannattoibixaorellanailactiveplantagainstsnakebites
AT abeldiazc evaluationofsomegrowthregulatorscombinationforcallusinductioninannattoibixaorellanailactiveplantagainstsnakebites
_version_ 1718420426336501760
spelling oai:doaj.org-article:5c12fc5844914b3aba719b9f525069882021-11-19T04:22:44ZEVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (<I>Bixa orellana</I> L.) ACTIVE PLANT AGAINST SNAKE BITES0121-40042145-2660https://doaj.org/article/5c12fc5844914b3aba719b9f525069882009-02-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/506https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 <!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} @font-face {font-family:Aldine401BT-RomanA; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"MS Mincho"; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 134676480 16 0 131073 0;} @font-face {font-family:Verdana; panose-1:2 11 6 4 3 5 4 4 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:536871559 0 0 0 415 0;} @font-face {font-family:"\@Aldine401BT-RomanA"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:0 134676480 16 0 131072 0;} @font-face {font-family:TimesNewRomanPS-ItalicMT; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:0; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} @font-face {font-family:SymbolMT; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"MS Mincho"; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:0 134676480 16 0 131072 0;} @font-face {font-family:"\@SymbolMT"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:128; mso-generic-font-family:auto; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:0 134676480 16 0 131072 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Recent evidences suggest annatto can be constituted in a therapeutic alternative by its direct and indirect influences against the poison of Bothrops asper, cause of most of snake bite in our country. The ex- vitro metabolic production of this plant is not obtained in early growth stages but it is possible to obtain it by means of cell cultures. This research evaluates some inductive calli combinations of plant segments of Bixa orellana L, like leaves, roots and stems grown in vitro, in order to get high induction rates and bigger amounts of tissue precursors of cellular suspensions, potentially producers of active metabolites. These explants, located in nutritious means without growth regulators, or with supplements of some combinations of them, are stored for eight weeks (25} 1º C and darkness) and the calli formation percentage tested in second, fifth and eighth week of growing. The calli generation in explants from roots is low, whereas in those from stems and leaves is favored with the treatment and with exposition time to the growth regulators. Regardless of the kind of explant used, the calli formation percentage is increased by the treatment that uses NAA (5 _M) and kinetine (4.64 _M), and showing values of 17,0 %, 58,9 % and 77,5 % for roots, stems and leaves respectively. Juan C. ALARCÓN P.Hader I. CASTAÑO P.Ligia L. CORRALES G.Silvia L. JIMÉNEZ R.Abel DÍAZ C.Universidad de AntioquiaarticlecalliBixa orellanaannatto.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 13, Iss 1 (2009)