Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs

An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples. The ic-ELISA, with optimized pH, methanol content an...

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Autores principales:	Xianlu Lei, Liguang Xu, Shanshan Song, Liqiang Liu, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	florfenicol ic-elisa immunochromatographic strip assay monoclonal antibody thiamphenicol Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/5c137e9e6a324abf99a9480f8d624628
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spelling	oai:doaj.org-article:5c137e9e6a324abf99a9480f8d6246282021-11-26T11:19:46ZDevelopment of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs0954-01051465-344310.1080/09540105.2017.1371114https://doaj.org/article/5c137e9e6a324abf99a9480f8d6246282018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1371114https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples. The ic-ELISA, with optimized pH, methanol content and sodium chloride content, exhibited an IC50 value of 0.2 ng/mL for FF and 0.27 ng/mL for TAP, with the working range of 0.05–0.77 and 0.05–1.42 ng/mL, respectively. The optimized ic-ELISA showed negligible cross-reactivity with other phenols and broad-spectrum antibiotics. The recoveries in egg samples using the ic-ELISA ranged from 84% to 115% with a coefficient of variation of less than 5%. Based on this monoclonal antibody, a rapid and ultrasensitive immunochromatographic strip assay was developed with a cutoff value of 1 ng/mL for FF and TAP. Our results indicated that both developed methods were highly useful for screening FF and TAP in eggs.Xianlu LeiLiguang XuShanshan SongLiqiang LiuHua KuangTaylor & Francis Grouparticleflorfenicolic-elisaimmunochromatographic strip assaymonoclonal antibodythiamphenicolAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 254-266 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	florfenicol ic-elisa immunochromatographic strip assay monoclonal antibody thiamphenicol Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	florfenicol ic-elisa immunochromatographic strip assay monoclonal antibody thiamphenicol Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Xianlu Lei Liguang Xu Shanshan Song Liqiang Liu Hua Kuang Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
description	An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples. The ic-ELISA, with optimized pH, methanol content and sodium chloride content, exhibited an IC50 value of 0.2 ng/mL for FF and 0.27 ng/mL for TAP, with the working range of 0.05–0.77 and 0.05–1.42 ng/mL, respectively. The optimized ic-ELISA showed negligible cross-reactivity with other phenols and broad-spectrum antibiotics. The recoveries in egg samples using the ic-ELISA ranged from 84% to 115% with a coefficient of variation of less than 5%. Based on this monoclonal antibody, a rapid and ultrasensitive immunochromatographic strip assay was developed with a cutoff value of 1 ng/mL for FF and TAP. Our results indicated that both developed methods were highly useful for screening FF and TAP in eggs.
format	article
author	Xianlu Lei Liguang Xu Shanshan Song Liqiang Liu Hua Kuang
author_facet	Xianlu Lei Liguang Xu Shanshan Song Liqiang Liu Hua Kuang
author_sort	Xianlu Lei
title	Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
title_short	Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
title_full	Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
title_fullStr	Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
title_full_unstemmed	Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
title_sort	development of an ultrasensitive ic-elisa and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/5c137e9e6a324abf99a9480f8d624628
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Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs

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