Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants

Stacey Markovic,1,* Jodi Belz,2,* Rajiv Kumar,3,4 Robert A Cormack,4 Srinivas Sridhar,3,4 Mark Niedre1 1Department of Electrical and Computer Engineering, 2Department of Bioengineering, 3Department of Physics, Northeastern University, Boston, MA, USA; 4Department of Radiation Oncology, Brigham and...

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Autores principales:	Markovic S, Belz J, Kumar R, Cormack RA, Sridhar S, Niedre M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	near infrared fluorescence imaging nanoparticles diffusion brachytherapy spacers Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5c1f10f7129e4785b2542d9b7283903d
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spelling	oai:doaj.org-article:5c1f10f7129e4785b2542d9b7283903d2021-12-02T00:38:50ZNear-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants1178-2013https://doaj.org/article/5c1f10f7129e4785b2542d9b7283903d2016-03-01T00:00:00Zhttps://www.dovepress.com/near-infrared-fluorescence-imaging-platform-for-quantifying-in-vivo-na-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Stacey Markovic,1,* Jodi Belz,2,* Rajiv Kumar,3,4 Robert A Cormack,4 Srinivas Sridhar,3,4 Mark Niedre1 1Department of Electrical and Computer Engineering, 2Department of Bioengineering, 3Department of Physics, Northeastern University, Boston, MA, USA; 4Department of Radiation Oncology, Brigham and Women’s Hospital, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA *These authors contributed equally to this work Abstract: Drug loaded implants are a new, versatile technology platform to deliver a localized payload of drugs for various disease models. One example is the implantable nanoplatform for chemo-radiation therapy where inert brachytherapy spacers are replaced by spacers doped with nanoparticles (NPs) loaded with chemotherapeutics and placed directly at the disease site for long-term localized drug delivery. However, it is difficult to directly validate and optimize the diffusion of these doped NPs in in vivo systems. To better study this drug release and diffusion, we developed a custom macroscopic fluorescence imaging system to visualize and quantify fluorescent NP diffusion from spacers in vivo. To validate the platform, we studied the release of free fluorophores, and 30 nm and 200 nm NPs conjugated with the same fluorophores as a model drug, in agar gel phantoms in vitro and in mice in vivo. Our data verified that the diffusion volume was NP size-dependent in all cases. Our near-infrared imaging system provides a method by which NP diffusion from implantable nanoplatform for chemo-radiation therapy spacers can be systematically optimized (eg, particle size or charge) thereby improving treatment efficacy of the platform. Keywords: optical imaging, fluorescence, drug delivery, brachytherapy, treatment monitoringMarkovic SBelz JKumar RCormack RASridhar SNiedre MDove Medical Pressarticlenear infraredfluorescence imagingnanoparticlesdiffusionbrachytherapy spacersMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1213-1223 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	near infrared fluorescence imaging nanoparticles diffusion brachytherapy spacers Medicine (General) R5-920
spellingShingle	near infrared fluorescence imaging nanoparticles diffusion brachytherapy spacers Medicine (General) R5-920 Markovic S Belz J Kumar R Cormack RA Sridhar S Niedre M Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
description	Stacey Markovic,1,* Jodi Belz,2,* Rajiv Kumar,3,4 Robert A Cormack,4 Srinivas Sridhar,3,4 Mark Niedre1 1Department of Electrical and Computer Engineering, 2Department of Bioengineering, 3Department of Physics, Northeastern University, Boston, MA, USA; 4Department of Radiation Oncology, Brigham and Women’s Hospital, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA *These authors contributed equally to this work Abstract: Drug loaded implants are a new, versatile technology platform to deliver a localized payload of drugs for various disease models. One example is the implantable nanoplatform for chemo-radiation therapy where inert brachytherapy spacers are replaced by spacers doped with nanoparticles (NPs) loaded with chemotherapeutics and placed directly at the disease site for long-term localized drug delivery. However, it is difficult to directly validate and optimize the diffusion of these doped NPs in in vivo systems. To better study this drug release and diffusion, we developed a custom macroscopic fluorescence imaging system to visualize and quantify fluorescent NP diffusion from spacers in vivo. To validate the platform, we studied the release of free fluorophores, and 30 nm and 200 nm NPs conjugated with the same fluorophores as a model drug, in agar gel phantoms in vitro and in mice in vivo. Our data verified that the diffusion volume was NP size-dependent in all cases. Our near-infrared imaging system provides a method by which NP diffusion from implantable nanoplatform for chemo-radiation therapy spacers can be systematically optimized (eg, particle size or charge) thereby improving treatment efficacy of the platform. Keywords: optical imaging, fluorescence, drug delivery, brachytherapy, treatment monitoring
format	article
author	Markovic S Belz J Kumar R Cormack RA Sridhar S Niedre M
author_facet	Markovic S Belz J Kumar R Cormack RA Sridhar S Niedre M
author_sort	Markovic S
title	Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
title_short	Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
title_full	Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
title_fullStr	Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
title_full_unstemmed	Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
title_sort	near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/5c1f10f7129e4785b2542d9b7283903d
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_version_	1718403556658118656

Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants

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