Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.

N-α-terminal acetylation is one of the most common, but least understood modifications of eukaryotic proteins. Although a high degree of conservation exists between the N-α-terminal acetylomes of plants and animals, very little information is available on this modification in plants. In yeast and hu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Almudena Ferrández-Ayela, Rosa Micol-Ponce, Ana Belén Sánchez-García, María Magdalena Alonso-Peral, José Luis Micol, María Rosa Ponce
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c331aa3f193467aab379a16d0bc04a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5c331aa3f193467aab379a16d0bc04a7
record_format	dspace
spelling	oai:doaj.org-article:5c331aa3f193467aab379a16d0bc04a72021-11-18T08:46:23ZMutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.1932-620310.1371/journal.pone.0080697https://doaj.org/article/5c331aa3f193467aab379a16d0bc04a72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244708/?tool=EBIhttps://doaj.org/toc/1932-6203N-α-terminal acetylation is one of the most common, but least understood modifications of eukaryotic proteins. Although a high degree of conservation exists between the N-α-terminal acetylomes of plants and animals, very little information is available on this modification in plants. In yeast and humans, N-α-acetyltransferase complexes include a single catalytic subunit and one or two auxiliary subunits. Here, we report the positional cloning of TRANSCURVATA2 (TCU2), which encodes the auxiliary subunit of the NatB N-α-acetyltransferase complex in Arabidopsis. The phenotypes of loss-of-function tcu2 alleles indicate that NatB complex activity is required for flowering time regulation and for leaf, inflorescence, flower, fruit and embryonic development. In double mutants, tcu2 alleles synergistically interact with alleles of ARGONAUTE10, which encodes a component of the microRNA machinery. In summary, NatB-mediated N-α-terminal acetylation of proteins is pleiotropically required for Arabidopsis development and seems to be functionally related to the microRNA pathway.Almudena Ferrández-AyelaRosa Micol-PonceAna Belén Sánchez-GarcíaMaría Magdalena Alonso-PeralJosé Luis MicolMaría Rosa PoncePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80697 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Almudena Ferrández-Ayela Rosa Micol-Ponce Ana Belén Sánchez-García María Magdalena Alonso-Peral José Luis Micol María Rosa Ponce Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
description	N-α-terminal acetylation is one of the most common, but least understood modifications of eukaryotic proteins. Although a high degree of conservation exists between the N-α-terminal acetylomes of plants and animals, very little information is available on this modification in plants. In yeast and humans, N-α-acetyltransferase complexes include a single catalytic subunit and one or two auxiliary subunits. Here, we report the positional cloning of TRANSCURVATA2 (TCU2), which encodes the auxiliary subunit of the NatB N-α-acetyltransferase complex in Arabidopsis. The phenotypes of loss-of-function tcu2 alleles indicate that NatB complex activity is required for flowering time regulation and for leaf, inflorescence, flower, fruit and embryonic development. In double mutants, tcu2 alleles synergistically interact with alleles of ARGONAUTE10, which encodes a component of the microRNA machinery. In summary, NatB-mediated N-α-terminal acetylation of proteins is pleiotropically required for Arabidopsis development and seems to be functionally related to the microRNA pathway.
format	article
author	Almudena Ferrández-Ayela Rosa Micol-Ponce Ana Belén Sánchez-García María Magdalena Alonso-Peral José Luis Micol María Rosa Ponce
author_facet	Almudena Ferrández-Ayela Rosa Micol-Ponce Ana Belén Sánchez-García María Magdalena Alonso-Peral José Luis Micol María Rosa Ponce
author_sort	Almudena Ferrández-Ayela
title	Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
title_short	Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
title_full	Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
title_fullStr	Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
title_full_unstemmed	Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
title_sort	mutation of an arabidopsis natb n-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5c331aa3f193467aab379a16d0bc04a7
work_keys_str_mv	AT almudenaferrandezayela mutationofanarabidopsisnatbnalphaterminalacetylationcomplexcomponentcausespleiotropicdevelopmentaldefects AT rosamicolponce mutationofanarabidopsisnatbnalphaterminalacetylationcomplexcomponentcausespleiotropicdevelopmentaldefects AT anabelensanchezgarcia mutationofanarabidopsisnatbnalphaterminalacetylationcomplexcomponentcausespleiotropicdevelopmentaldefects AT mariamagdalenaalonsoperal mutationofanarabidopsisnatbnalphaterminalacetylationcomplexcomponentcausespleiotropicdevelopmentaldefects AT joseluismicol mutationofanarabidopsisnatbnalphaterminalacetylationcomplexcomponentcausespleiotropicdevelopmentaldefects AT mariarosaponce mutationofanarabidopsisnatbnalphaterminalacetylationcomplexcomponentcausespleiotropicdevelopmentaldefects
_version_	1718421322693869568

Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.

Ejemplares similares