Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p

Temporal lobe epilepsy (TLE) often occurs in childhood and is the most common type of epilepsy. Studies have confirmed that long non-coding RNAs (lncRNAs) can affect the progression of neurological diseases. This study explored the expression level of lncRNA TUG1 in TLE children and its clinical sig...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chunlian Li, Xiaojing Zheng, Pingping Liu, Meilian Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	lncrna tug1 mir-199a-3p proliferation apoptosis hippocampus neuron temporal lobe epilepsy Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5c3756a298cf40cf816c1236e9fe1822
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5c3756a298cf40cf816c1236e9fe1822
record_format	dspace
spelling	oai:doaj.org-article:5c3756a298cf40cf816c1236e9fe18222021-11-26T11:19:49ZClinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p2165-59792165-598710.1080/21655979.2021.2001904https://doaj.org/article/5c3756a298cf40cf816c1236e9fe18222021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001904https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Temporal lobe epilepsy (TLE) often occurs in childhood and is the most common type of epilepsy. Studies have confirmed that long non-coding RNAs (lncRNAs) can affect the progression of neurological diseases. This study explored the expression level of lncRNA TUG1 in TLE children and its clinical significance and investigated its role in hippocampal neurons. 86 healthy individuals and 88 TLE children were recruited. The expressions of lncRNA TUG1 and miR-199a-3p in serum were detected by qRT-PCR. Hippocampal neurons were treated with non-Mg2+ to establish TLE cell model. MTT assay and flow cytometry assay was used to detect the effect of lncRNA TUG1 on the proliferation and apoptosis of hippocampal neurons. A dual-luciferase reporter assay was done to confirm the target relationship. The expression of lncRNA TUG1 was increased in TLE children compared with the control group. The diagnostic potential was reflected by the receiver operator characteristic (ROC) curve, with the AUC of 0.915 at the cut-off value of 1.256. Elevated levels of TUG1 were detected in TLE cell models, and TUG1 knockout could enhance cell activity and inhibit cell apoptosis. MiR-199a-3p was the target of TUG1. Clinically, the serum miR-199a-3p levels showed a negative association with TUG1. LncRNA TUG1 may be a biomarker of TLE diagnosis in children, and can regulate hippocampal neuron cell activity and apoptosis via sponging miR-199a-3p.Chunlian LiXiaojing ZhengPingping LiuMeilian LiTaylor & Francis Grouparticlelncrna tug1mir-199a-3pproliferationapoptosishippocampus neurontemporal lobe epilepsyBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncrna tug1 mir-199a-3p proliferation apoptosis hippocampus neuron temporal lobe epilepsy Biotechnology TP248.13-248.65
spellingShingle	lncrna tug1 mir-199a-3p proliferation apoptosis hippocampus neuron temporal lobe epilepsy Biotechnology TP248.13-248.65 Chunlian Li Xiaojing Zheng Pingping Liu Meilian Li Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p
description	Temporal lobe epilepsy (TLE) often occurs in childhood and is the most common type of epilepsy. Studies have confirmed that long non-coding RNAs (lncRNAs) can affect the progression of neurological diseases. This study explored the expression level of lncRNA TUG1 in TLE children and its clinical significance and investigated its role in hippocampal neurons. 86 healthy individuals and 88 TLE children were recruited. The expressions of lncRNA TUG1 and miR-199a-3p in serum were detected by qRT-PCR. Hippocampal neurons were treated with non-Mg2+ to establish TLE cell model. MTT assay and flow cytometry assay was used to detect the effect of lncRNA TUG1 on the proliferation and apoptosis of hippocampal neurons. A dual-luciferase reporter assay was done to confirm the target relationship. The expression of lncRNA TUG1 was increased in TLE children compared with the control group. The diagnostic potential was reflected by the receiver operator characteristic (ROC) curve, with the AUC of 0.915 at the cut-off value of 1.256. Elevated levels of TUG1 were detected in TLE cell models, and TUG1 knockout could enhance cell activity and inhibit cell apoptosis. MiR-199a-3p was the target of TUG1. Clinically, the serum miR-199a-3p levels showed a negative association with TUG1. LncRNA TUG1 may be a biomarker of TLE diagnosis in children, and can regulate hippocampal neuron cell activity and apoptosis via sponging miR-199a-3p.
format	article
author	Chunlian Li Xiaojing Zheng Pingping Liu Meilian Li
author_facet	Chunlian Li Xiaojing Zheng Pingping Liu Meilian Li
author_sort	Chunlian Li
title	Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p
title_short	Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p
title_full	Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p
title_fullStr	Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p
title_full_unstemmed	Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p
title_sort	clinical value of lncrna tug1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging mir-199a-3p
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/5c3756a298cf40cf816c1236e9fe1822
work_keys_str_mv	AT chunlianli clinicalvalueoflncrnatug1intemporallobeepilepsyanditsroleintheproliferationofhippocampusneuronviaspongingmir199a3p AT xiaojingzheng clinicalvalueoflncrnatug1intemporallobeepilepsyanditsroleintheproliferationofhippocampusneuronviaspongingmir199a3p AT pingpingliu clinicalvalueoflncrnatug1intemporallobeepilepsyanditsroleintheproliferationofhippocampusneuronviaspongingmir199a3p AT meilianli clinicalvalueoflncrnatug1intemporallobeepilepsyanditsroleintheproliferationofhippocampusneuronviaspongingmir199a3p
_version_	1718409472588644352

Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p

Ejemplares similares