Genetic control of resistance to Trypanosoma brucei brucei infection in mice.

<h4>Background</h4>Trypanosoma brucei brucei infects livestock, with severe effects in horses and dogs. Mouse strains differ greatly in susceptibility to this parasite. However, no genes controlling these differences were mapped.<h4>Methods</h4>We studied the genetic control...

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Autores principales:	Matyáš Síma, Helena Havelková, Lei Quan, Milena Svobodová, Taťána Jarošíková, Jarmila Vojtíšková, Alphons P M Stassen, Peter Demant, Marie Lipoldová
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/5c390e675c114dcd956262f108f03994
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spelling	oai:doaj.org-article:5c390e675c114dcd956262f108f039942021-11-18T09:13:18ZGenetic control of resistance to Trypanosoma brucei brucei infection in mice.1935-27271935-273510.1371/journal.pntd.0001173https://doaj.org/article/5c390e675c114dcd956262f108f039942011-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21666791/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Trypanosoma brucei brucei infects livestock, with severe effects in horses and dogs. Mouse strains differ greatly in susceptibility to this parasite. However, no genes controlling these differences were mapped.<h4>Methods</h4>We studied the genetic control of survival after T. b. brucei infection using recombinant congenic (RC) strains, which have a high mapping power. Each RC strain of BALB/c-c-STS/A (CcS/Dem) series contains a different random subset of 12.5% genes from the parental "donor" strain STS/A and 87.5% genes from the "background" strain BALB/c. Although BALB/c and STS/A mice are similarly susceptible to T. b. brucei, the RC strain CcS-11 is more susceptible than either of them. We analyzed genetics of survival in T. b. brucei-infected F(2) hybrids between BALB/c and CcS-11. CcS-11 strain carries STS-derived segments on eight chromosomes. They were genotyped in the F(2) hybrid mice and their linkage with survival was tested by analysis of variance.<h4>Results</h4>We mapped four Tbbr (Trypanosoma brucei brucei response) loci that influence survival after T. b. brucei infection. Tbbr1 (chromosome 3) and Tbbr2 (chromosome 12) have effects on survival independent of inter-genic interactions (main effects). Tbbr3 (chromosome 7) influences survival in interaction with Tbbr4 (chromosome 19). Tbbr2 is located on a segment 2.15 Mb short that contains only 26 genes.<h4>Conclusion</h4>This study presents the first identification of chromosomal loci controlling susceptibility to T. b. brucei infection. While mapping in F(2) hybrids of inbred strains usually has a precision of 40-80 Mb, in RC strains we mapped Tbbr2 to a 2.15 Mb segment containing only 26 genes, which will enable an effective search for the candidate gene. Definition of susceptibility genes will improve the understanding of pathways and genetic diversity underlying the disease and may result in new strategies to overcome the active subversion of the immune system by T. b. brucei.Matyáš SímaHelena HavelkováLei QuanMilena SvobodováTaťána JarošíkováJarmila VojtíškováAlphons P M StassenPeter DemantMarie LipoldováPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1173 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Matyáš Síma Helena Havelková Lei Quan Milena Svobodová Taťána Jarošíková Jarmila Vojtíšková Alphons P M Stassen Peter Demant Marie Lipoldová Genetic control of resistance to Trypanosoma brucei brucei infection in mice.
description	<h4>Background</h4>Trypanosoma brucei brucei infects livestock, with severe effects in horses and dogs. Mouse strains differ greatly in susceptibility to this parasite. However, no genes controlling these differences were mapped.<h4>Methods</h4>We studied the genetic control of survival after T. b. brucei infection using recombinant congenic (RC) strains, which have a high mapping power. Each RC strain of BALB/c-c-STS/A (CcS/Dem) series contains a different random subset of 12.5% genes from the parental "donor" strain STS/A and 87.5% genes from the "background" strain BALB/c. Although BALB/c and STS/A mice are similarly susceptible to T. b. brucei, the RC strain CcS-11 is more susceptible than either of them. We analyzed genetics of survival in T. b. brucei-infected F(2) hybrids between BALB/c and CcS-11. CcS-11 strain carries STS-derived segments on eight chromosomes. They were genotyped in the F(2) hybrid mice and their linkage with survival was tested by analysis of variance.<h4>Results</h4>We mapped four Tbbr (Trypanosoma brucei brucei response) loci that influence survival after T. b. brucei infection. Tbbr1 (chromosome 3) and Tbbr2 (chromosome 12) have effects on survival independent of inter-genic interactions (main effects). Tbbr3 (chromosome 7) influences survival in interaction with Tbbr4 (chromosome 19). Tbbr2 is located on a segment 2.15 Mb short that contains only 26 genes.<h4>Conclusion</h4>This study presents the first identification of chromosomal loci controlling susceptibility to T. b. brucei infection. While mapping in F(2) hybrids of inbred strains usually has a precision of 40-80 Mb, in RC strains we mapped Tbbr2 to a 2.15 Mb segment containing only 26 genes, which will enable an effective search for the candidate gene. Definition of susceptibility genes will improve the understanding of pathways and genetic diversity underlying the disease and may result in new strategies to overcome the active subversion of the immune system by T. b. brucei.
format	article
author	Matyáš Síma Helena Havelková Lei Quan Milena Svobodová Taťána Jarošíková Jarmila Vojtíšková Alphons P M Stassen Peter Demant Marie Lipoldová
author_facet	Matyáš Síma Helena Havelková Lei Quan Milena Svobodová Taťána Jarošíková Jarmila Vojtíšková Alphons P M Stassen Peter Demant Marie Lipoldová
author_sort	Matyáš Síma
title	Genetic control of resistance to Trypanosoma brucei brucei infection in mice.
title_short	Genetic control of resistance to Trypanosoma brucei brucei infection in mice.
title_full	Genetic control of resistance to Trypanosoma brucei brucei infection in mice.
title_fullStr	Genetic control of resistance to Trypanosoma brucei brucei infection in mice.
title_full_unstemmed	Genetic control of resistance to Trypanosoma brucei brucei infection in mice.
title_sort	genetic control of resistance to trypanosoma brucei brucei infection in mice.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/5c390e675c114dcd956262f108f03994
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Genetic control of resistance to Trypanosoma brucei brucei infection in mice.

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