Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities

ABSTRACT Syntrophic interactions between organohalide-respiring and fermentative microorganisms are critical for effective bioremediation of halogenated compounds. This work investigated the effect of ammonium concentration (up to 4 g liter−1 NH4+-N) on trichloroethene-reducing Dehalococcoides mccar...

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Autores principales:	Anca G. Delgado, Devyn Fajardo-Williams, Kylie L. Kegerreis, Prathap Parameswaran, Rosa Krajmalnik-Brown
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Dehalococcoides mccartyi Geobacter ammonia fermentation organohalide respiration trichloroethene Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5c42ed39bc9f47ab94c82e2fb0a13336
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spelling	oai:doaj.org-article:5c42ed39bc9f47ab94c82e2fb0a133362021-11-15T15:21:22ZImpact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities10.1128/mSphere.00053-162379-5042https://doaj.org/article/5c42ed39bc9f47ab94c82e2fb0a133362016-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00053-16https://doaj.org/toc/2379-5042ABSTRACT Syntrophic interactions between organohalide-respiring and fermentative microorganisms are critical for effective bioremediation of halogenated compounds. This work investigated the effect of ammonium concentration (up to 4 g liter−1 NH4+-N) on trichloroethene-reducing Dehalococcoides mccartyi and Geobacteraceae in microbial communities fed lactate and methanol. We found that production of ethene by D. mccartyi occurred in mineral medium containing ≤2 g liter−1 NH4+-N and in landfill leachate. For the partial reduction of trichloroethene (TCE) to cis-dichloroethene (cis-DCE) at ≥1 g liter−1 NH4+-N, organohalide-respiring dynamics shifted from D. mccartyi and Geobacteraceae to mainly D. mccartyi. An increasing concentration of ammonium was coupled to lower metabolic rates, longer lag times, and lower gene abundances for all microbial processes studied. The methanol fermentation pathway to acetate and H2 was conserved, regardless of the ammonium concentration provided. However, lactate fermentation shifted from propionic to acetogenic at concentrations of ≥2 g liter−1 NH4+-N. Our study findings strongly support a tolerance of D. mccartyi to high ammonium concentrations, highlighting the feasibility of organohalide respiration in ammonium-contaminated subsurface environments. IMPORTANCE Contamination with ammonium and chlorinated solvents has been reported in numerous subsurface environments, and these chemicals bring significant challenges for in situ bioremediation. Dehalococcoides mccartyi is able to reduce the chlorinated solvent trichloroethene to the nontoxic end product ethene. Fermentative bacteria are of central importance for organohalide respiration and bioremediation to provide D. mccartyi with H2, their electron donor, acetate, their carbon source, and other micronutrients. In this study, we found that high concentrations of ammonium negatively correlated with rates of trichloroethene reductive dehalogenation and fermentation. However, detoxification of trichloroethene to nontoxic ethene occurred even at ammonium concentrations typical of those found in animal waste (up to 2 g liter−1 NH4+-N). To date, hundreds of subsurface environments have been bioremediated through the unique metabolic capability of D. mccartyi. These findings extend our knowledge of D. mccartyi and provide insight for bioremediation of sites contaminated with chlorinated solvents and ammonium.Anca G. DelgadoDevyn Fajardo-WilliamsKylie L. KegerreisPrathap ParameswaranRosa Krajmalnik-BrownAmerican Society for MicrobiologyarticleDehalococcoides mccartyiGeobacterammoniafermentationorganohalide respirationtrichloroetheneMicrobiologyQR1-502ENmSphere, Vol 1, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dehalococcoides mccartyi Geobacter ammonia fermentation organohalide respiration trichloroethene Microbiology QR1-502
spellingShingle	Dehalococcoides mccartyi Geobacter ammonia fermentation organohalide respiration trichloroethene Microbiology QR1-502 Anca G. Delgado Devyn Fajardo-Williams Kylie L. Kegerreis Prathap Parameswaran Rosa Krajmalnik-Brown Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
description	ABSTRACT Syntrophic interactions between organohalide-respiring and fermentative microorganisms are critical for effective bioremediation of halogenated compounds. This work investigated the effect of ammonium concentration (up to 4 g liter−1 NH4+-N) on trichloroethene-reducing Dehalococcoides mccartyi and Geobacteraceae in microbial communities fed lactate and methanol. We found that production of ethene by D. mccartyi occurred in mineral medium containing ≤2 g liter−1 NH4+-N and in landfill leachate. For the partial reduction of trichloroethene (TCE) to cis-dichloroethene (cis-DCE) at ≥1 g liter−1 NH4+-N, organohalide-respiring dynamics shifted from D. mccartyi and Geobacteraceae to mainly D. mccartyi. An increasing concentration of ammonium was coupled to lower metabolic rates, longer lag times, and lower gene abundances for all microbial processes studied. The methanol fermentation pathway to acetate and H2 was conserved, regardless of the ammonium concentration provided. However, lactate fermentation shifted from propionic to acetogenic at concentrations of ≥2 g liter−1 NH4+-N. Our study findings strongly support a tolerance of D. mccartyi to high ammonium concentrations, highlighting the feasibility of organohalide respiration in ammonium-contaminated subsurface environments. IMPORTANCE Contamination with ammonium and chlorinated solvents has been reported in numerous subsurface environments, and these chemicals bring significant challenges for in situ bioremediation. Dehalococcoides mccartyi is able to reduce the chlorinated solvent trichloroethene to the nontoxic end product ethene. Fermentative bacteria are of central importance for organohalide respiration and bioremediation to provide D. mccartyi with H2, their electron donor, acetate, their carbon source, and other micronutrients. In this study, we found that high concentrations of ammonium negatively correlated with rates of trichloroethene reductive dehalogenation and fermentation. However, detoxification of trichloroethene to nontoxic ethene occurred even at ammonium concentrations typical of those found in animal waste (up to 2 g liter−1 NH4+-N). To date, hundreds of subsurface environments have been bioremediated through the unique metabolic capability of D. mccartyi. These findings extend our knowledge of D. mccartyi and provide insight for bioremediation of sites contaminated with chlorinated solvents and ammonium.
format	article
author	Anca G. Delgado Devyn Fajardo-Williams Kylie L. Kegerreis Prathap Parameswaran Rosa Krajmalnik-Brown
author_facet	Anca G. Delgado Devyn Fajardo-Williams Kylie L. Kegerreis Prathap Parameswaran Rosa Krajmalnik-Brown
author_sort	Anca G. Delgado
title	Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
title_short	Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
title_full	Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
title_fullStr	Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
title_full_unstemmed	Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
title_sort	impact of ammonium on syntrophic organohalide-respiring and fermenting microbial communities
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/5c42ed39bc9f47ab94c82e2fb0a13336
work_keys_str_mv	AT ancagdelgado impactofammoniumonsyntrophicorganohaliderespiringandfermentingmicrobialcommunities AT devynfajardowilliams impactofammoniumonsyntrophicorganohaliderespiringandfermentingmicrobialcommunities AT kylielkegerreis impactofammoniumonsyntrophicorganohaliderespiringandfermentingmicrobialcommunities AT prathapparameswaran impactofammoniumonsyntrophicorganohaliderespiringandfermentingmicrobialcommunities AT rosakrajmalnikbrown impactofammoniumonsyntrophicorganohaliderespiringandfermentingmicrobialcommunities
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Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities

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