Multiple gene substitution by Target-AID base-editing technology in tomato

Abstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Johan Hunziker, Keiji Nishida, Akihiko Kondo, Sanae Kishimoto, Tohru Ariizumi, Hiroshi Ezura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c452f7144bc471a93a6b09ad8de7141
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5c452f7144bc471a93a6b09ad8de7141
record_format	dspace
spelling	oai:doaj.org-article:5c452f7144bc471a93a6b09ad8de71412021-12-02T16:08:59ZMultiple gene substitution by Target-AID base-editing technology in tomato10.1038/s41598-020-77379-22045-2322https://doaj.org/article/5c452f7144bc471a93a6b09ad8de71412020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77379-2https://doaj.org/toc/2045-2322Abstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions.Johan HunzikerKeiji NishidaAkihiko KondoSanae KishimotoTohru AriizumiHiroshi EzuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johan Hunziker Keiji Nishida Akihiko Kondo Sanae Kishimoto Tohru Ariizumi Hiroshi Ezura Multiple gene substitution by Target-AID base-editing technology in tomato
description	Abstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions.
format	article
author	Johan Hunziker Keiji Nishida Akihiko Kondo Sanae Kishimoto Tohru Ariizumi Hiroshi Ezura
author_facet	Johan Hunziker Keiji Nishida Akihiko Kondo Sanae Kishimoto Tohru Ariizumi Hiroshi Ezura
author_sort	Johan Hunziker
title	Multiple gene substitution by Target-AID base-editing technology in tomato
title_short	Multiple gene substitution by Target-AID base-editing technology in tomato
title_full	Multiple gene substitution by Target-AID base-editing technology in tomato
title_fullStr	Multiple gene substitution by Target-AID base-editing technology in tomato
title_full_unstemmed	Multiple gene substitution by Target-AID base-editing technology in tomato
title_sort	multiple gene substitution by target-aid base-editing technology in tomato
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5c452f7144bc471a93a6b09ad8de7141
work_keys_str_mv	AT johanhunziker multiplegenesubstitutionbytargetaidbaseeditingtechnologyintomato AT keijinishida multiplegenesubstitutionbytargetaidbaseeditingtechnologyintomato AT akihikokondo multiplegenesubstitutionbytargetaidbaseeditingtechnologyintomato AT sanaekishimoto multiplegenesubstitutionbytargetaidbaseeditingtechnologyintomato AT tohruariizumi multiplegenesubstitutionbytargetaidbaseeditingtechnologyintomato AT hiroshiezura multiplegenesubstitutionbytargetaidbaseeditingtechnologyintomato
_version_	1718384470982131712

Multiple gene substitution by Target-AID base-editing technology in tomato

Ejemplares similares