Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.

<h4>Objective</h4>The aim of this study was to evaluate the expression of a panel of genes involved in toxicology in response to styrene exposure at levels below the occupational standard setting.<h4>Methods</h4>Workers in a fiber glass boat industry were evaluated for a pane...

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Autores principales:	Elisabetta Strafella, Massimo Bracci, Sara Staffolani, Nicola Manzella, Daniele Giantomasi, Matteo Valentino, Monica Amati, Marco Tomasetti, Lory Santarelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c4db8caae844a9e8f7e71b2cc71381d
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spelling	oai:doaj.org-article:5c4db8caae844a9e8f7e71b2cc71381d2021-11-18T08:54:10ZOccupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.1932-620310.1371/journal.pone.0075401https://doaj.org/article/5c4db8caae844a9e8f7e71b2cc71381d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086524/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>The aim of this study was to evaluate the expression of a panel of genes involved in toxicology in response to styrene exposure at levels below the occupational standard setting.<h4>Methods</h4>Workers in a fiber glass boat industry were evaluated for a panel of stress- and toxicity-related genes and associated with biochemical parameters related to hepatic injury. Urinary styrene metabolites (MA+PGA) of subjects and environmental sampling data collected for air at workplace were used to estimate styrene exposure.<h4>Results</h4>Expression array analysis revealed massive upregulation of genes encoding stress-responsive proteins (HSPA1L, EGR1, IL-6, IL-1β, TNSF10 and TNFα) in the styrene-exposed group; the levels of cytokines released were further confirmed in serum. The exposed workers were then stratified by styrene exposure levels. EGR1 gene upregulation paralleled the expression and transcriptional protein levels of IL-6, TNSF10 and TNFα in styrene exposed workers, even at low level. The activation of the EGR1 pathway observed at low-styrene exposure was associated with a slight increase of hepatic markers found in highly exposed subjects, even though they were within normal range. The ALT and AST levels were not affected by alcohol consumption, and positively correlated with urinary styrene metabolites as evaluated by multiple regression analysis.<h4>Conclusion</h4>The pro-inflammatory cytokines IL-6 and TNFα are the primary mediators of processes involved in the hepatic injury response and regeneration. Here, we show that styrene induced stress responsive genes involved in cytoprotection and cytotoxicity at low-exposure, that proceed to a mild subclinical hepatic toxicity at high-styrene exposure.Elisabetta StrafellaMassimo BracciSara StaffolaniNicola ManzellaDaniele GiantomasiMatteo ValentinoMonica AmatiMarco TomasettiLory SantarelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75401 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisabetta Strafella Massimo Bracci Sara Staffolani Nicola Manzella Daniele Giantomasi Matteo Valentino Monica Amati Marco Tomasetti Lory Santarelli Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
description	<h4>Objective</h4>The aim of this study was to evaluate the expression of a panel of genes involved in toxicology in response to styrene exposure at levels below the occupational standard setting.<h4>Methods</h4>Workers in a fiber glass boat industry were evaluated for a panel of stress- and toxicity-related genes and associated with biochemical parameters related to hepatic injury. Urinary styrene metabolites (MA+PGA) of subjects and environmental sampling data collected for air at workplace were used to estimate styrene exposure.<h4>Results</h4>Expression array analysis revealed massive upregulation of genes encoding stress-responsive proteins (HSPA1L, EGR1, IL-6, IL-1β, TNSF10 and TNFα) in the styrene-exposed group; the levels of cytokines released were further confirmed in serum. The exposed workers were then stratified by styrene exposure levels. EGR1 gene upregulation paralleled the expression and transcriptional protein levels of IL-6, TNSF10 and TNFα in styrene exposed workers, even at low level. The activation of the EGR1 pathway observed at low-styrene exposure was associated with a slight increase of hepatic markers found in highly exposed subjects, even though they were within normal range. The ALT and AST levels were not affected by alcohol consumption, and positively correlated with urinary styrene metabolites as evaluated by multiple regression analysis.<h4>Conclusion</h4>The pro-inflammatory cytokines IL-6 and TNFα are the primary mediators of processes involved in the hepatic injury response and regeneration. Here, we show that styrene induced stress responsive genes involved in cytoprotection and cytotoxicity at low-exposure, that proceed to a mild subclinical hepatic toxicity at high-styrene exposure.
format	article
author	Elisabetta Strafella Massimo Bracci Sara Staffolani Nicola Manzella Daniele Giantomasi Matteo Valentino Monica Amati Marco Tomasetti Lory Santarelli
author_facet	Elisabetta Strafella Massimo Bracci Sara Staffolani Nicola Manzella Daniele Giantomasi Matteo Valentino Monica Amati Marco Tomasetti Lory Santarelli
author_sort	Elisabetta Strafella
title	Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
title_short	Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
title_full	Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
title_fullStr	Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
title_full_unstemmed	Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
title_sort	occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5c4db8caae844a9e8f7e71b2cc71381d
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Occupational styrene exposure induces stress-responsive genes involved in cytoprotective and cytotoxic activities.

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