Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response

Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response

Shuyun Liu,1 Lanlan Zhang,2 Jingqiu Cheng,1 Yanrong Lu,1 Jingping Liu1 1Key Laboratory of Transplant Engineering and Immunology, West China Hospital, 2Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, People’s Republic of China Abstract: Inflammat...

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Autores principales: Liu S, Zhang L, Cheng J, Lu Y, Liu J
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
self-assembling peptide
islet transplantation
inflammation
HGF
slow release
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/5c5dc6df813c44e292b5ba94729a5fba
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spelling oai:doaj.org-article:5c5dc6df813c44e292b5ba94729a5fba2021-12-02T03:58:33ZSustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response1178-2013https://doaj.org/article/5c5dc6df813c44e292b5ba94729a5fba2016-09-01T00:00:00Zhttps://www.dovepress.com/sustained-release-of-hepatocyte-growth-factor-by-cationic-self-assembl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shuyun Liu,1 Lanlan Zhang,2 Jingqiu Cheng,1 Yanrong Lu,1 Jingping Liu1 1Key Laboratory of Transplant Engineering and Immunology, West China Hospital, 2Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, People’s Republic of China Abstract: Inflammatory response is a major cause of grafts dysfunction in islet transplantation. Hepatocyte growth factor (HGF) had shown anti-inflammatory activity in multiple diseases. In this study, we aim to deliver HGF by self-assembling peptide/heparin (SAP/Hep) hybrid gel to protect β-cell from inflammatory injury. The morphological and slow release properties of SAPs were analyzed. Rat INS-1 β-cell line was treated with tumor necrosis factor α in vitro and transplanted into rat kidney capsule in vivo, and the viability, apoptosis, function, and inflammation of β-cells were evaluated. Cationic KLD1R and KLD2R self-assembled to nanofiber hydrogel, which showed higher binding affinity for Hep and HGF because of electrostatic interaction. Slow release of HGF from cationic SAP/Hep gel is a two-step mechanism involving binding affinity with Hep and molecular diffusion. In vitro and in vivo results showed that HGF-loaded KLD2R/Hep gel promoted β-cell survival and insulin secretion, and inhibited cell apoptosis, cytokine release, T-cell infiltration, and activation of NFκB/p38 MAPK pathways in β-cells. This study suggested that SAP/Hep gel is a promising carrier for local delivery of bioactive proteins in islet transplantation. Keywords: self-assembling peptide, islet transplantation, inflammation, HGF, slow releaseLiu SZhang LCheng JLu YLiu JDove Medical Pressarticleself-assembling peptideislet transplantationinflammationHGFslow releaseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4875-4890 (2016)
institution DOAJ
collection DOAJ
language EN
topic self-assembling peptide
islet transplantation
inflammation
HGF
slow release
Medicine (General)
R5-920
spellingShingle self-assembling peptide
islet transplantation
inflammation
HGF
slow release
Medicine (General)
R5-920
Liu S
Zhang L
Cheng J
Lu Y
Liu J
Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
description Shuyun Liu,1 Lanlan Zhang,2 Jingqiu Cheng,1 Yanrong Lu,1 Jingping Liu1 1Key Laboratory of Transplant Engineering and Immunology, West China Hospital, 2Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu, People’s Republic of China Abstract: Inflammatory response is a major cause of grafts dysfunction in islet transplantation. Hepatocyte growth factor (HGF) had shown anti-inflammatory activity in multiple diseases. In this study, we aim to deliver HGF by self-assembling peptide/heparin (SAP/Hep) hybrid gel to protect β-cell from inflammatory injury. The morphological and slow release properties of SAPs were analyzed. Rat INS-1 β-cell line was treated with tumor necrosis factor α in vitro and transplanted into rat kidney capsule in vivo, and the viability, apoptosis, function, and inflammation of β-cells were evaluated. Cationic KLD1R and KLD2R self-assembled to nanofiber hydrogel, which showed higher binding affinity for Hep and HGF because of electrostatic interaction. Slow release of HGF from cationic SAP/Hep gel is a two-step mechanism involving binding affinity with Hep and molecular diffusion. In vitro and in vivo results showed that HGF-loaded KLD2R/Hep gel promoted β-cell survival and insulin secretion, and inhibited cell apoptosis, cytokine release, T-cell infiltration, and activation of NFκB/p38 MAPK pathways in β-cells. This study suggested that SAP/Hep gel is a promising carrier for local delivery of bioactive proteins in islet transplantation. Keywords: self-assembling peptide, islet transplantation, inflammation, HGF, slow release
format article
author Liu S
Zhang L
Cheng J
Lu Y
Liu J
author_facet Liu S
Zhang L
Cheng J
Lu Y
Liu J
author_sort Liu S
title Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
title_short Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
title_full Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
title_fullStr Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
title_full_unstemmed Sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
title_sort sustained release of hepatocyte growth factor by cationic self-assembling peptide/heparin hybrid hydrogel improves β-cell survival and function through modulating inflammatory response
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/5c5dc6df813c44e292b5ba94729a5fba
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AT zhangl sustainedreleaseofhepatocytegrowthfactorbycationicselfassemblingpeptideheparinhybridhydrogelimprovesbetacellsurvivalandfunctionthroughmodulatinginflammatoryresponse
AT chengj sustainedreleaseofhepatocytegrowthfactorbycationicselfassemblingpeptideheparinhybridhydrogelimprovesbetacellsurvivalandfunctionthroughmodulatinginflammatoryresponse
AT luy sustainedreleaseofhepatocytegrowthfactorbycationicselfassemblingpeptideheparinhybridhydrogelimprovesbetacellsurvivalandfunctionthroughmodulatinginflammatoryresponse
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