PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology

In this research, Analytical Quality by Design (AQbD) methodology was used to develop the HILIC-PDA-CAD method for the determination of magnesium, pyridoxine and thiamine content in a dietary supplement. Experiments were conducted on the Dionex Ulitimate 3000 HPLC system with PDA and CAD detectors....

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Autores principales:	Rmandić Milena, Rađenović Miloš, Stanković Jovana, Protić Ana, Otašević Biljana, Malenović Anđelija
Formato:	article
Lenguaje:	SR
Publicado:	Pharmaceutical Association of Serbia, Belgrade, Serbia 2021
Materias:	hilic-pda-cad magnesium pyridoxine thiamine aqbd Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/5c644bf3aa284f508663722a5f37a925
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spelling	oai:doaj.org-article:5c644bf3aa284f508663722a5f37a9252021-12-05T18:01:41ZPDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology0004-19632217-876710.5937/arhfarm71-32093https://doaj.org/article/5c644bf3aa284f508663722a5f37a9252021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632105378R.pdfhttps://doaj.org/toc/0004-1963https://doaj.org/toc/2217-8767In this research, Analytical Quality by Design (AQbD) methodology was used to develop the HILIC-PDA-CAD method for the determination of magnesium, pyridoxine and thiamine content in a dietary supplement. Experiments were conducted on the Dionex Ulitimate 3000 HPLC system with PDA and CAD detectors. Separations were performed on the ZIC-HILIC PEEK column (100 × 4.6 mm, 5 mm) with a mixture of acetonitrile, acetone and ammonium acetate/ammonium formate aqueous solution (48:12:40, v/v/v) as the mobile phase. The detection was performed at 220 nm. The optimal settings of CAD were defined with the one-factor-at-a-time approach: evaporation temperature 50°C, filter constant 10 s, pressure gas 60 psi. The influence of qualitative (salt type) and quantitative factors (pH and salt concentration) on the magnesium retention factor, k Mg and the selectivity factor between pyridoxine and chloride anion, a B6/Cl-, was tested using the I-optimal design. The design space was defined by Monte Carlo simulations and model coefficients' errors were propagated with the aim of identifying the conditions that meet the following criteria: k Mg <4 and a B6/Cl->3.5, with the probability p=95%. From the derived 2D-Design Space graph, 95 mM of ammonium formate pH 4.4 was selected as the optimal composition of the aqueous phase. The method was validated and its reliability in routine application was confirmed.Rmandić MilenaRađenović MilošStanković JovanaProtić AnaOtašević BiljanaMalenović AnđelijaPharmaceutical Association of Serbia, Belgrade, Serbiaarticlehilic-pda-cadmagnesiumpyridoxinethiamineaqbdPharmacy and materia medicaRS1-441SRArhiv za farmaciju, Vol 71, Iss 5, Pp 378-392 (2021)
institution	DOAJ
collection	DOAJ
language	SR
topic	hilic-pda-cad magnesium pyridoxine thiamine aqbd Pharmacy and materia medica RS1-441
spellingShingle	hilic-pda-cad magnesium pyridoxine thiamine aqbd Pharmacy and materia medica RS1-441 Rmandić Milena Rađenović Miloš Stanković Jovana Protić Ana Otašević Biljana Malenović Anđelija PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
description	In this research, Analytical Quality by Design (AQbD) methodology was used to develop the HILIC-PDA-CAD method for the determination of magnesium, pyridoxine and thiamine content in a dietary supplement. Experiments were conducted on the Dionex Ulitimate 3000 HPLC system with PDA and CAD detectors. Separations were performed on the ZIC-HILIC PEEK column (100 × 4.6 mm, 5 mm) with a mixture of acetonitrile, acetone and ammonium acetate/ammonium formate aqueous solution (48:12:40, v/v/v) as the mobile phase. The detection was performed at 220 nm. The optimal settings of CAD were defined with the one-factor-at-a-time approach: evaporation temperature 50°C, filter constant 10 s, pressure gas 60 psi. The influence of qualitative (salt type) and quantitative factors (pH and salt concentration) on the magnesium retention factor, k Mg and the selectivity factor between pyridoxine and chloride anion, a B6/Cl-, was tested using the I-optimal design. The design space was defined by Monte Carlo simulations and model coefficients' errors were propagated with the aim of identifying the conditions that meet the following criteria: k Mg <4 and a B6/Cl->3.5, with the probability p=95%. From the derived 2D-Design Space graph, 95 mM of ammonium formate pH 4.4 was selected as the optimal composition of the aqueous phase. The method was validated and its reliability in routine application was confirmed.
format	article
author	Rmandić Milena Rađenović Miloš Stanković Jovana Protić Ana Otašević Biljana Malenović Anđelija
author_facet	Rmandić Milena Rađenović Miloš Stanković Jovana Protić Ana Otašević Biljana Malenović Anđelija
author_sort	Rmandić Milena
title	PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
title_short	PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
title_full	PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
title_fullStr	PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
title_full_unstemmed	PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
title_sort	pda-cad method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology
publisher	Pharmaceutical Association of Serbia, Belgrade, Serbia
publishDate	2021
url	https://doaj.org/article/5c644bf3aa284f508663722a5f37a925
work_keys_str_mv	AT rmandicmilena pdacadmethodforthedeterminationofmagnesiumpyridoxineandthiamineinadietarysupplementsupportedbyanalyticalqualitybydesignmethodology AT rađenovicmilos pdacadmethodforthedeterminationofmagnesiumpyridoxineandthiamineinadietarysupplementsupportedbyanalyticalqualitybydesignmethodology AT stankovicjovana pdacadmethodforthedeterminationofmagnesiumpyridoxineandthiamineinadietarysupplementsupportedbyanalyticalqualitybydesignmethodology AT proticana pdacadmethodforthedeterminationofmagnesiumpyridoxineandthiamineinadietarysupplementsupportedbyanalyticalqualitybydesignmethodology AT otasevicbiljana pdacadmethodforthedeterminationofmagnesiumpyridoxineandthiamineinadietarysupplementsupportedbyanalyticalqualitybydesignmethodology AT malenovicanđelija pdacadmethodforthedeterminationofmagnesiumpyridoxineandthiamineinadietarysupplementsupportedbyanalyticalqualitybydesignmethodology
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PDA-CAD method for the determination of magnesium, pyridoxine and thiamine in a dietary supplement supported by analytical quality by design methodology

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