Genome-wide identification of Argonautes in Solanaceae with emphasis on potato

Abstract Regulatory small RNAs (sRNAs) play important roles in many fundamental processes in plant biology such as development, fertilization and stress responses. The AGO protein family has here a central importance in gene regulation based on their capacity to associate with sRNAs followed by mRNA...

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Autores principales:	Zhen Liao, Kristian Persson Hodén, Ravi Kumar Singh, Christina Dixelius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c6b624418a44c96bd1c413a1fe249f9
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spelling	oai:doaj.org-article:5c6b624418a44c96bd1c413a1fe249f92021-12-02T11:42:13ZGenome-wide identification of Argonautes in Solanaceae with emphasis on potato10.1038/s41598-020-77593-y2045-2322https://doaj.org/article/5c6b624418a44c96bd1c413a1fe249f92020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77593-yhttps://doaj.org/toc/2045-2322Abstract Regulatory small RNAs (sRNAs) play important roles in many fundamental processes in plant biology such as development, fertilization and stress responses. The AGO protein family has here a central importance in gene regulation based on their capacity to associate with sRNAs followed by mRNA targeting in a sequence-complementary manner. The present study explored Argonautes (AGOs) in the Solanaceae family, with emphasis on potato, Solanum tuberosum (St). A genome-wide monitoring was performed to provide a deeper insight into gene families, genomic localization, gene structure and expression profile against the potato late blight pathogen Phytophthora infestans. Among 15 species in the Solanaceae family we found a variation from ten AGOs in Nicotiana obtusifolia to 17 in N. tabacum. Comprehensive analyses of AGO phylogeny revealed duplication of AGO1, AGO10 and AGO4 paralogs during early radiation of Solanaceae. Fourteen AGOs were identified in potato. Orthologs of AGO8 and AGO9 were missing in the potato genome. However, AGO15 earlier annotated in tomato was identified. StAGO15 differs from the other paralogs having residues of different physico-chemical properties at functionally important amino acid positions. Upon pathogen challenge StAGO15 was significantly activated and hence may play a prominent role in sRNA-based regulation of potato defense.Zhen LiaoKristian Persson HodénRavi Kumar SinghChristina DixeliusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhen Liao Kristian Persson Hodén Ravi Kumar Singh Christina Dixelius Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
description	Abstract Regulatory small RNAs (sRNAs) play important roles in many fundamental processes in plant biology such as development, fertilization and stress responses. The AGO protein family has here a central importance in gene regulation based on their capacity to associate with sRNAs followed by mRNA targeting in a sequence-complementary manner. The present study explored Argonautes (AGOs) in the Solanaceae family, with emphasis on potato, Solanum tuberosum (St). A genome-wide monitoring was performed to provide a deeper insight into gene families, genomic localization, gene structure and expression profile against the potato late blight pathogen Phytophthora infestans. Among 15 species in the Solanaceae family we found a variation from ten AGOs in Nicotiana obtusifolia to 17 in N. tabacum. Comprehensive analyses of AGO phylogeny revealed duplication of AGO1, AGO10 and AGO4 paralogs during early radiation of Solanaceae. Fourteen AGOs were identified in potato. Orthologs of AGO8 and AGO9 were missing in the potato genome. However, AGO15 earlier annotated in tomato was identified. StAGO15 differs from the other paralogs having residues of different physico-chemical properties at functionally important amino acid positions. Upon pathogen challenge StAGO15 was significantly activated and hence may play a prominent role in sRNA-based regulation of potato defense.
format	article
author	Zhen Liao Kristian Persson Hodén Ravi Kumar Singh Christina Dixelius
author_facet	Zhen Liao Kristian Persson Hodén Ravi Kumar Singh Christina Dixelius
author_sort	Zhen Liao
title	Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
title_short	Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
title_full	Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
title_fullStr	Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
title_full_unstemmed	Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
title_sort	genome-wide identification of argonautes in solanaceae with emphasis on potato
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5c6b624418a44c96bd1c413a1fe249f9
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Genome-wide identification of Argonautes in Solanaceae with emphasis on potato

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