Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions

Abstract Background Mosquitoes are important vectors of disease-causing organisms such as filarial worms, malaria parasites, and arboviruses endemic to sub-Saharan Africa including Nigeria. Malaria is a disease caused by a plasmodium parasite, transmitted by the bite of infected mosquitoes and is no...

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Autores principales:	Oluyinka A. Iyiola, Aishat T. Kamaldeen-Ibrahim, Rahmat D. Shaibu, Olalere Shittu, Temitope O. Fadipe, Olukayode J. Adelaja, Muyideen K. Tijani, Hajarat A. Afolabi
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Mosquitoes Cytochrome oxidase subunit I Intergenic spacer region Genetic diversity Zoology QL1-991
Acceso en línea:	https://doaj.org/article/5c884cc6cfea4a01be8b60d31887e23f
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spelling	oai:doaj.org-article:5c884cc6cfea4a01be8b60d31887e23f2021-11-14T12:24:47ZMolecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions10.1186/s41936-021-00252-92090-990Xhttps://doaj.org/article/5c884cc6cfea4a01be8b60d31887e23f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s41936-021-00252-9https://doaj.org/toc/2090-990XAbstract Background Mosquitoes are important vectors of disease-causing organisms such as filarial worms, malaria parasites, and arboviruses endemic to sub-Saharan Africa including Nigeria. Malaria is a disease caused by a plasmodium parasite, transmitted by the bite of infected mosquitoes and is no doubt a public health concern. There is limited information on the genetic diversity of mosquitoes in Nigeria. This is necessary because information about the genetic diversity of mosquitoes is a very important step towards vector control and management with the aim to mitigate or eliminate burden resulting from malaria and other diseases caused by mosquitoes. In the present study, we investigated the genetic variability and relatedness of mosquitoes based on the DNA sequences of the mitochondrial cytochrome oxidase subunit I (COI) and ribosomal intergenic non-coding spacer gene regions (IGS). Mosquitoes were collected from five different states in Northcentral Nigeria, they were morphologically identified using standard keys and genomic DNA was extracted. The specific regions of interests were amplified, and the PCR products were then sequenced. Results PCR was able to successfully amplify the expected amplicon sizes of COI and IGS sequences (710 and 169) base pairs, respectively. For COI sequence, pairwise genetic distance between mosquito species ranged from 0.00 to 0.17 in the COI sequences. The pairwise genetic distance among Culex, Aedes and Anopheles species in the IGS sequences ranged from 0.000 to 0.118. Phylogenetic analysis of the sequences of the mitochondrial cytochrome oxidase subunit showed that there was genetic diversity amongst the different mosquito species sampled. It effectively showed marked differences between Culicine and Anopheline mosquitoes. Conclusions The ribosomal IGS primers used for this study only amplified Anopheles spp. However, it revealed that there is diversity among the Anopheles gambiae and Anopheles arabiensis samples collected. This study concludes that the mitochondrial COI and ribosomal IGS gene regions are reliable markers for mosquito genetic diversity study and will surely yield a reliable result for molecular diversity assessment of mosquito species.Oluyinka A. IyiolaAishat T. Kamaldeen-IbrahimRahmat D. ShaibuOlalere ShittuTemitope O. FadipeOlukayode J. AdelajaMuyideen K. TijaniHajarat A. AfolabiSpringerOpenarticleMosquitoesCytochrome oxidase subunit IIntergenic spacer regionGenetic diversityZoologyQL1-991ENJournal of Basic and Applied Zoology, Vol 82, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mosquitoes Cytochrome oxidase subunit I Intergenic spacer region Genetic diversity Zoology QL1-991
spellingShingle	Mosquitoes Cytochrome oxidase subunit I Intergenic spacer region Genetic diversity Zoology QL1-991 Oluyinka A. Iyiola Aishat T. Kamaldeen-Ibrahim Rahmat D. Shaibu Olalere Shittu Temitope O. Fadipe Olukayode J. Adelaja Muyideen K. Tijani Hajarat A. Afolabi Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions
description	Abstract Background Mosquitoes are important vectors of disease-causing organisms such as filarial worms, malaria parasites, and arboviruses endemic to sub-Saharan Africa including Nigeria. Malaria is a disease caused by a plasmodium parasite, transmitted by the bite of infected mosquitoes and is no doubt a public health concern. There is limited information on the genetic diversity of mosquitoes in Nigeria. This is necessary because information about the genetic diversity of mosquitoes is a very important step towards vector control and management with the aim to mitigate or eliminate burden resulting from malaria and other diseases caused by mosquitoes. In the present study, we investigated the genetic variability and relatedness of mosquitoes based on the DNA sequences of the mitochondrial cytochrome oxidase subunit I (COI) and ribosomal intergenic non-coding spacer gene regions (IGS). Mosquitoes were collected from five different states in Northcentral Nigeria, they were morphologically identified using standard keys and genomic DNA was extracted. The specific regions of interests were amplified, and the PCR products were then sequenced. Results PCR was able to successfully amplify the expected amplicon sizes of COI and IGS sequences (710 and 169) base pairs, respectively. For COI sequence, pairwise genetic distance between mosquito species ranged from 0.00 to 0.17 in the COI sequences. The pairwise genetic distance among Culex, Aedes and Anopheles species in the IGS sequences ranged from 0.000 to 0.118. Phylogenetic analysis of the sequences of the mitochondrial cytochrome oxidase subunit showed that there was genetic diversity amongst the different mosquito species sampled. It effectively showed marked differences between Culicine and Anopheline mosquitoes. Conclusions The ribosomal IGS primers used for this study only amplified Anopheles spp. However, it revealed that there is diversity among the Anopheles gambiae and Anopheles arabiensis samples collected. This study concludes that the mitochondrial COI and ribosomal IGS gene regions are reliable markers for mosquito genetic diversity study and will surely yield a reliable result for molecular diversity assessment of mosquito species.
format	article
author	Oluyinka A. Iyiola Aishat T. Kamaldeen-Ibrahim Rahmat D. Shaibu Olalere Shittu Temitope O. Fadipe Olukayode J. Adelaja Muyideen K. Tijani Hajarat A. Afolabi
author_facet	Oluyinka A. Iyiola Aishat T. Kamaldeen-Ibrahim Rahmat D. Shaibu Olalere Shittu Temitope O. Fadipe Olukayode J. Adelaja Muyideen K. Tijani Hajarat A. Afolabi
author_sort	Oluyinka A. Iyiola
title	Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions
title_short	Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions
title_full	Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions
title_fullStr	Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions
title_full_unstemmed	Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions
title_sort	molecular characterization and phylogenetic analysis of collected mosquitoes (diptera: culicidae) from northcentral nigeria using mitochondrial coi and ribosomal igs gene regions
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/5c884cc6cfea4a01be8b60d31887e23f
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Molecular characterization and phylogenetic analysis of collected mosquitoes (Diptera: Culicidae) from Northcentral Nigeria using mitochondrial COI and ribosomal IGS gene regions

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