Distinct lung microbiota associate with HIV-associated chronic lung disease in children

Abstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a cross-sectional study of 202 HIV-infected children...

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Autores principales:	Sudha Bhadriraju, Douglas W. Fadrosh, Meera K. Shenoy, Din L. Lin, Kole V. Lynch, Kathryn McCauley, Rashida A. Ferrand, Edith D. Majonga, Grace McHugh, Laurence Huang, Susan V. Lynch, John Z. Metcalfe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c889e806c114c2e94ed9a46227d7dc5
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spelling	oai:doaj.org-article:5c889e806c114c2e94ed9a46227d7dc52021-12-02T18:51:06ZDistinct lung microbiota associate with HIV-associated chronic lung disease in children10.1038/s41598-020-73085-12045-2322https://doaj.org/article/5c889e806c114c2e94ed9a46227d7dc52020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73085-1https://doaj.org/toc/2045-2322Abstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a cross-sectional study of 202 HIV-infected children aged 6–16 years in Harare, Zimbabwe, we determined the association of sputum microbiota composition (using 16S ribosomal RNA V4 gene region sequencing) with CLD defined using clinical, spirometric, or radiographic criteria. Forty-two percent of children were determined to have CLD according to our definition. Dirichlet multinomial mixtures identified four compositionally distinct sputum microbiota structures. Patients whose sputum microbiota was dominated by Haemophilus, Moraxella or Neisseria (HMN) were at 1.5 times higher risk of CLD than those with Streptococcus or Prevotella (SP)-dominated microbiota (RR = 1.48, p = 0.035). Cell-free products of HMN sputum microbiota induced features of epithelial disruption and inflammatory gene expression in vitro, indicating enhanced pathogenic potential of these CLD-associated microbiota. Thus, HIV-positive children harbor distinct sputum microbiota, with those dominated by Haemophilus, Moraxella or Neisseria associated with enhanced pathogenesis in vitro and clinical CLD.Sudha BhadrirajuDouglas W. FadroshMeera K. ShenoyDin L. LinKole V. LynchKathryn McCauleyRashida A. FerrandEdith D. MajongaGrace McHughLaurence HuangSusan V. LynchJohn Z. MetcalfeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sudha Bhadriraju Douglas W. Fadrosh Meera K. Shenoy Din L. Lin Kole V. Lynch Kathryn McCauley Rashida A. Ferrand Edith D. Majonga Grace McHugh Laurence Huang Susan V. Lynch John Z. Metcalfe Distinct lung microbiota associate with HIV-associated chronic lung disease in children
description	Abstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a cross-sectional study of 202 HIV-infected children aged 6–16 years in Harare, Zimbabwe, we determined the association of sputum microbiota composition (using 16S ribosomal RNA V4 gene region sequencing) with CLD defined using clinical, spirometric, or radiographic criteria. Forty-two percent of children were determined to have CLD according to our definition. Dirichlet multinomial mixtures identified four compositionally distinct sputum microbiota structures. Patients whose sputum microbiota was dominated by Haemophilus, Moraxella or Neisseria (HMN) were at 1.5 times higher risk of CLD than those with Streptococcus or Prevotella (SP)-dominated microbiota (RR = 1.48, p = 0.035). Cell-free products of HMN sputum microbiota induced features of epithelial disruption and inflammatory gene expression in vitro, indicating enhanced pathogenic potential of these CLD-associated microbiota. Thus, HIV-positive children harbor distinct sputum microbiota, with those dominated by Haemophilus, Moraxella or Neisseria associated with enhanced pathogenesis in vitro and clinical CLD.
format	article
author	Sudha Bhadriraju Douglas W. Fadrosh Meera K. Shenoy Din L. Lin Kole V. Lynch Kathryn McCauley Rashida A. Ferrand Edith D. Majonga Grace McHugh Laurence Huang Susan V. Lynch John Z. Metcalfe
author_facet	Sudha Bhadriraju Douglas W. Fadrosh Meera K. Shenoy Din L. Lin Kole V. Lynch Kathryn McCauley Rashida A. Ferrand Edith D. Majonga Grace McHugh Laurence Huang Susan V. Lynch John Z. Metcalfe
author_sort	Sudha Bhadriraju
title	Distinct lung microbiota associate with HIV-associated chronic lung disease in children
title_short	Distinct lung microbiota associate with HIV-associated chronic lung disease in children
title_full	Distinct lung microbiota associate with HIV-associated chronic lung disease in children
title_fullStr	Distinct lung microbiota associate with HIV-associated chronic lung disease in children
title_full_unstemmed	Distinct lung microbiota associate with HIV-associated chronic lung disease in children
title_sort	distinct lung microbiota associate with hiv-associated chronic lung disease in children
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5c889e806c114c2e94ed9a46227d7dc5
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Distinct lung microbiota associate with HIV-associated chronic lung disease in children

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