RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection

ABSTRACT The cytoplasmic helicase RIG-I is an established sensor for viral 5′-triphosphorylated RNA species. Recently, RIG-I was also implicated in the detection of intracellular bacteria. However, little is known about the host cell specificity of this process and the bacterial pathogen-associated...

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Autores principales:	Mirco Schmolke, Jenish R. Patel, Elisa de Castro, Maria T. Sánchez-Aparicio, Melissa B. Uccellini, Jennifer C. Miller, Balaji Manicassamy, Takashi Satoh, Taro Kawai, Shizuo Akira, Miriam Merad, Adolfo García-Sastre
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5c8c7580d0d14579892faa77373b07c6
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spelling	oai:doaj.org-article:5c8c7580d0d14579892faa77373b07c62021-11-15T15:45:13ZRIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection10.1128/mBio.01006-142150-7511https://doaj.org/article/5c8c7580d0d14579892faa77373b07c62014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01006-14https://doaj.org/toc/2150-7511ABSTRACT The cytoplasmic helicase RIG-I is an established sensor for viral 5′-triphosphorylated RNA species. Recently, RIG-I was also implicated in the detection of intracellular bacteria. However, little is known about the host cell specificity of this process and the bacterial pathogen-associated molecular pattern (PAMP) that activates RIG-I. Here we show that RNA of Salmonella enterica serovar Typhimurium activates production of beta interferon in a RIG-I-dependent fashion only in nonphagocytic cells. In phagocytic cells, RIG-I is obsolete for detection of Salmonella infection. We further demonstrate that Salmonella mRNA reaches the cytoplasm during infection and is thus accessible for RIG-I. The results from next-generation sequencing analysis of RIG-I-associated RNA suggest that coding bacterial mRNAs represent the activating PAMP. IMPORTANCE S. Typhimurium is a major food-borne pathogen. After fecal-oral transmission, it can infect epithelial cells in the gut as well as immune cells (mainly macrophages, dendritic cells, and M cells). The innate host immune system relies on a growing number of sensors that detect pathogen-associated molecular patterns (PAMPs) to launch a first broad-spectrum response to invading pathogens. Successful detection of a given pathogen depends on colocalization of host sensors and PAMPs as well as potential countermeasures of the pathogen during infection. RIG-I-like helicases were mainly associated with detection of RNA viruses. Our work shows that S. Typhimurium is detected by RIG-I during infection specifically in nonimmune cells.Mirco SchmolkeJenish R. PatelElisa de CastroMaria T. Sánchez-AparicioMelissa B. UccelliniJennifer C. MillerBalaji ManicassamyTakashi SatohTaro KawaiShizuo AkiraMiriam MeradAdolfo García-SastreAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Mirco Schmolke Jenish R. Patel Elisa de Castro Maria T. Sánchez-Aparicio Melissa B. Uccellini Jennifer C. Miller Balaji Manicassamy Takashi Satoh Taro Kawai Shizuo Akira Miriam Merad Adolfo García-Sastre RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection
description	ABSTRACT The cytoplasmic helicase RIG-I is an established sensor for viral 5′-triphosphorylated RNA species. Recently, RIG-I was also implicated in the detection of intracellular bacteria. However, little is known about the host cell specificity of this process and the bacterial pathogen-associated molecular pattern (PAMP) that activates RIG-I. Here we show that RNA of Salmonella enterica serovar Typhimurium activates production of beta interferon in a RIG-I-dependent fashion only in nonphagocytic cells. In phagocytic cells, RIG-I is obsolete for detection of Salmonella infection. We further demonstrate that Salmonella mRNA reaches the cytoplasm during infection and is thus accessible for RIG-I. The results from next-generation sequencing analysis of RIG-I-associated RNA suggest that coding bacterial mRNAs represent the activating PAMP. IMPORTANCE S. Typhimurium is a major food-borne pathogen. After fecal-oral transmission, it can infect epithelial cells in the gut as well as immune cells (mainly macrophages, dendritic cells, and M cells). The innate host immune system relies on a growing number of sensors that detect pathogen-associated molecular patterns (PAMPs) to launch a first broad-spectrum response to invading pathogens. Successful detection of a given pathogen depends on colocalization of host sensors and PAMPs as well as potential countermeasures of the pathogen during infection. RIG-I-like helicases were mainly associated with detection of RNA viruses. Our work shows that S. Typhimurium is detected by RIG-I during infection specifically in nonimmune cells.
format	article
author	Mirco Schmolke Jenish R. Patel Elisa de Castro Maria T. Sánchez-Aparicio Melissa B. Uccellini Jennifer C. Miller Balaji Manicassamy Takashi Satoh Taro Kawai Shizuo Akira Miriam Merad Adolfo García-Sastre
author_facet	Mirco Schmolke Jenish R. Patel Elisa de Castro Maria T. Sánchez-Aparicio Melissa B. Uccellini Jennifer C. Miller Balaji Manicassamy Takashi Satoh Taro Kawai Shizuo Akira Miriam Merad Adolfo García-Sastre
author_sort	Mirco Schmolke
title	RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection
title_short	RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection
title_full	RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection
title_fullStr	RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection
title_full_unstemmed	RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection
title_sort	rig-i detects mrna of intracellular <named-content content-type="genus-species">salmonella enterica</named-content> serovar typhimurium during bacterial infection
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/5c8c7580d0d14579892faa77373b07c6
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RIG-I Detects mRNA of Intracellular <named-content content-type="genus-species">Salmonella enterica</named-content> Serovar Typhimurium during Bacterial Infection

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