In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

Bacterial diarrhea remains a global health problem, especially in developing tropical countries. Moreover, dysbiosis caused by diarrheagenic bacteria and inappropriate antimicrobial treatment has been associated with intestinal carcinogenesis. Despite the rich tradition of the use of herbs for the t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tomas Kudera, Barbora Fiserova, Marie Korytakova, Ivo Doskocil, Hana Salmonova, Edgardo E. Tulin, Samnang Nguon, Marlito M. Bande, Ladislav Kokoska
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	diarrhea Cambodia Philippines medicinal plant antibacterial anticancer Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5c8d3f4b16284842a2c5fbf46366928d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5c8d3f4b16284842a2c5fbf46366928d
record_format	dspace
spelling	oai:doaj.org-article:5c8d3f4b16284842a2c5fbf46366928d2021-12-01T05:43:43ZIn Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment1663-981210.3389/fphar.2021.746808https://doaj.org/article/5c8d3f4b16284842a2c5fbf46366928d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.746808/fullhttps://doaj.org/toc/1663-9812Bacterial diarrhea remains a global health problem, especially in developing tropical countries. Moreover, dysbiosis caused by diarrheagenic bacteria and inappropriate antimicrobial treatment has been associated with intestinal carcinogenesis. Despite the rich tradition of the use of herbs for the treatment of gastrointestinal disorders in Cambodian and Philippine folk medicine, many of them have not yet been systematically studied for their in vitro selective inhibitory effects on intestinal bacteria and cells. In the present study, in vitro inhibitory activities of 35 ethanolic extracts derived from 32 Cambodian and Philippine medicinal plants were determined by broth microdilution method against 12 pathogenic bacteria. Furthermore, cytotoxicity against intestinal cancer cells (Caco-2 and HT-29) using thiazolyl blue tetrazolium bromide cytotoxicity assay and safety to six beneficial intestinal bacteria (bifidobacteria and lactobacilli) and intestinal normal cells (FHs 74 Int) were determined for the antimicrobially active extracts. Selectivity indices (SIs) were calculated among the averages of minimum inhibitory concentrations (MICs), half-maximal inhibitory concentrations (IC50), and 80% inhibitory concentrations of proliferation (IC80) for each type of the tested agents. The extracts of Artocarpus blancoi (Elmer) Merr. (Moraceae), Ancistrocladus tectorius (Lour.) Merr. (Ancistrocladaceae), and Pentacme siamensis (Miq.) Kurz (Dipterocarpaceae) produced significant growth-inhibitory effects (MICs = 32–512 μg/ml) against intestinal pathogenic bacteria at the concentrations nontoxic to normal intestinal cells (IC80 values >512 μg/ml; SIs = 0.11–0.2). Moreover, the extract of P. siamensis (Miq.) Kurz was relatively safe to beneficial bacteria (MICs ≥512 μg/ml; SI = 0.1), and together with A. blancoi (Elmer) Merr., they selectively inhibited intestinal cancer cells (IC50 values ≥51.98 ± 19.79 μg/ml; SIs = 0.3 and 0.6). Finally, a strong selective antiproliferative effect on cancer cells (IC50 values 37.89 ± 2.68 to 130.89 ± 13.99 μg/ml; SIs = 0.5) was exerted by Ehretia microphylla Lam. (Boraginaceae), Lagerstroemia cochinchinensis Pierre ex Gagnep. (Lythraceae), and Melastoma saigonense (Kuntze) Merr. (Melastomataceae) (leaves with flower buds). The results suggest that the above-mentioned species are promising materials for the development of new selective antibacterial and antiproliferative agents for the treatment of infectious diarrhea and associated intestinal cancer diseases. However, further research is needed regarding the isolation and identification of their active constituents.Tomas KuderaBarbora FiserovaMarie KorytakovaIvo DoskocilHana SalmonovaEdgardo E. TulinSamnang NguonMarlito M. BandeLadislav KokoskaFrontiers Media S.A.articlediarrheaCambodiaPhilippinesmedicinal plantantibacterialanticancerTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diarrhea Cambodia Philippines medicinal plant antibacterial anticancer Therapeutics. Pharmacology RM1-950
spellingShingle	diarrhea Cambodia Philippines medicinal plant antibacterial anticancer Therapeutics. Pharmacology RM1-950 Tomas Kudera Barbora Fiserova Marie Korytakova Ivo Doskocil Hana Salmonova Edgardo E. Tulin Samnang Nguon Marlito M. Bande Ladislav Kokoska In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment
description	Bacterial diarrhea remains a global health problem, especially in developing tropical countries. Moreover, dysbiosis caused by diarrheagenic bacteria and inappropriate antimicrobial treatment has been associated with intestinal carcinogenesis. Despite the rich tradition of the use of herbs for the treatment of gastrointestinal disorders in Cambodian and Philippine folk medicine, many of them have not yet been systematically studied for their in vitro selective inhibitory effects on intestinal bacteria and cells. In the present study, in vitro inhibitory activities of 35 ethanolic extracts derived from 32 Cambodian and Philippine medicinal plants were determined by broth microdilution method against 12 pathogenic bacteria. Furthermore, cytotoxicity against intestinal cancer cells (Caco-2 and HT-29) using thiazolyl blue tetrazolium bromide cytotoxicity assay and safety to six beneficial intestinal bacteria (bifidobacteria and lactobacilli) and intestinal normal cells (FHs 74 Int) were determined for the antimicrobially active extracts. Selectivity indices (SIs) were calculated among the averages of minimum inhibitory concentrations (MICs), half-maximal inhibitory concentrations (IC50), and 80% inhibitory concentrations of proliferation (IC80) for each type of the tested agents. The extracts of Artocarpus blancoi (Elmer) Merr. (Moraceae), Ancistrocladus tectorius (Lour.) Merr. (Ancistrocladaceae), and Pentacme siamensis (Miq.) Kurz (Dipterocarpaceae) produced significant growth-inhibitory effects (MICs = 32–512 μg/ml) against intestinal pathogenic bacteria at the concentrations nontoxic to normal intestinal cells (IC80 values >512 μg/ml; SIs = 0.11–0.2). Moreover, the extract of P. siamensis (Miq.) Kurz was relatively safe to beneficial bacteria (MICs ≥512 μg/ml; SI = 0.1), and together with A. blancoi (Elmer) Merr., they selectively inhibited intestinal cancer cells (IC50 values ≥51.98 ± 19.79 μg/ml; SIs = 0.3 and 0.6). Finally, a strong selective antiproliferative effect on cancer cells (IC50 values 37.89 ± 2.68 to 130.89 ± 13.99 μg/ml; SIs = 0.5) was exerted by Ehretia microphylla Lam. (Boraginaceae), Lagerstroemia cochinchinensis Pierre ex Gagnep. (Lythraceae), and Melastoma saigonense (Kuntze) Merr. (Melastomataceae) (leaves with flower buds). The results suggest that the above-mentioned species are promising materials for the development of new selective antibacterial and antiproliferative agents for the treatment of infectious diarrhea and associated intestinal cancer diseases. However, further research is needed regarding the isolation and identification of their active constituents.
format	article
author	Tomas Kudera Barbora Fiserova Marie Korytakova Ivo Doskocil Hana Salmonova Edgardo E. Tulin Samnang Nguon Marlito M. Bande Ladislav Kokoska
author_facet	Tomas Kudera Barbora Fiserova Marie Korytakova Ivo Doskocil Hana Salmonova Edgardo E. Tulin Samnang Nguon Marlito M. Bande Ladislav Kokoska
author_sort	Tomas Kudera
title	In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment
title_short	In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment
title_full	In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment
title_fullStr	In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment
title_full_unstemmed	In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment
title_sort	in vitro selective antibacterial and antiproliferative effects of ethanolic extracts from cambodian and philippine plants used in folk medicine for diarrhea treatment
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5c8d3f4b16284842a2c5fbf46366928d
work_keys_str_mv	AT tomaskudera invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT barborafiserova invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT mariekorytakova invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT ivodoskocil invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT hanasalmonova invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT edgardoetulin invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT samnangnguon invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT marlitombande invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment AT ladislavkokoska invitroselectiveantibacterialandantiproliferativeeffectsofethanolicextractsfromcambodianandphilippineplantsusedinfolkmedicinefordiarrheatreatment
_version_	1718405476602871808

In Vitro Selective Antibacterial and Antiproliferative Effects of Ethanolic Extracts from Cambodian and Philippine Plants Used in Folk Medicine for Diarrhea Treatment

Ejemplares similares