Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension

Abstract Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Masashi Tachikawa, Nobuhiro Morone, Yosuke Senju, Tadao Sugiura, Kyoko Hanawa-Suetsugu, Atsushi Mochizuki, Shiro Suetsugu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c8f8d097fbc4ca2b8fdc07dd9c7afa5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5c8f8d097fbc4ca2b8fdc07dd9c7afa5
record_format	dspace
spelling	oai:doaj.org-article:5c8f8d097fbc4ca2b8fdc07dd9c7afa52021-12-02T11:40:14ZMeasurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension10.1038/s41598-017-08259-52045-2322https://doaj.org/article/5c8f8d097fbc4ca2b8fdc07dd9c7afa52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08259-5https://doaj.org/toc/2045-2322Abstract Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.Masashi TachikawaNobuhiro MoroneYosuke SenjuTadao SugiuraKyoko Hanawa-SuetsuguAtsushi MochizukiShiro SuetsuguNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masashi Tachikawa Nobuhiro Morone Yosuke Senju Tadao Sugiura Kyoko Hanawa-Suetsugu Atsushi Mochizuki Shiro Suetsugu Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
description	Abstract Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates.
format	article
author	Masashi Tachikawa Nobuhiro Morone Yosuke Senju Tadao Sugiura Kyoko Hanawa-Suetsugu Atsushi Mochizuki Shiro Suetsugu
author_facet	Masashi Tachikawa Nobuhiro Morone Yosuke Senju Tadao Sugiura Kyoko Hanawa-Suetsugu Atsushi Mochizuki Shiro Suetsugu
author_sort	Masashi Tachikawa
title	Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
title_short	Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
title_full	Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
title_fullStr	Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
title_full_unstemmed	Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
title_sort	measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5c8f8d097fbc4ca2b8fdc07dd9c7afa5
work_keys_str_mv	AT masashitachikawa measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension AT nobuhiromorone measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension AT yosukesenju measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension AT tadaosugiura measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension AT kyokohanawasuetsugu measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension AT atsushimochizuki measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension AT shirosuetsugu measurementofcaveolin1densitiesinthecellmembraneforquantificationofcaveolardeformationafterexposuretohypotonicmembranetension
_version_	1718395643967307776

Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension

Ejemplares similares