Optical birefringence of liquid crystals for label-free optical biosensing diagnosis

Tan Tai Nguyen,1 Gyeo-Re Han,2 Chang-Hyun Jang,1,2 Heongkyu Ju1,3,4 1Department of Bionano Technology, 2Department of Nano-Chemistry, 3Department of Nano-Physics, Gachon University, Seongnam-City, 4Neuroscience Institute, Gil Hospital, Incheon, South Korea Purpose: We present a polarization-sensit...

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Autores principales:	Nguyen TT, Han GR, Jang CH, Ju H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5c9b30130d9b4cc0a3e9d2dd2766e66b
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spelling	oai:doaj.org-article:5c9b30130d9b4cc0a3e9d2dd2766e66b2021-12-02T03:00:39ZOptical birefringence of liquid crystals for label-free optical biosensing diagnosis1178-2013https://doaj.org/article/5c9b30130d9b4cc0a3e9d2dd2766e66b2015-08-01T00:00:00Zhttp://www.dovepress.com/optical-birefringence-of-liquid-crystals-for-label-free-optical-biosen-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tan Tai Nguyen,1 Gyeo-Re Han,2 Chang-Hyun Jang,1,2 Heongkyu Ju1,3,4 1Department of Bionano Technology, 2Department of Nano-Chemistry, 3Department of Nano-Physics, Gachon University, Seongnam-City, 4Neuroscience Institute, Gil Hospital, Incheon, South Korea Purpose: We present a polarization-sensitive optical detection platform for label-free quantitative optical biosensing diagnosis using liquid crystals (LCs). This is capable of determining quantitatively the optical birefringence of optical cells containing LCs, whose orientation depends on the immobilized biomolecules.Patients and methods: This technique uses a polarization-dependent double-port detection without any polarizer at a single wavelength and removes the need of aligning optical cells of LCs in the azimuthal direction, with respect to the light path through the optical cell. Thus, this technique enables a stand-alone detection in a relatively compact format without an additional optical instrument, such as a retardation compensator, a Michael–Levy chart, and a spectrophotometer, in order to determine the optical birefringence quantitatively.Results: We demonstrate that bovine serum albumin immobilized on the gold surface of the cell hybrid interfaces that support both homeotropic and planar anchoring of LCs causes optical phase retardation change which can be determined quantitatively. We also provide estimation of the zenithal orientation of LCs near the gold surface of the hybrid interfaces, based on the phase retardation determined. The estimated limit of bovine serum albumin detection is approximately 2.1 µM.Conclusion: This optical technique with LCs can serve an optical platform for label-free quantitative diagnosis of proteins in a real time manner. Keywords: biological sensors, LC orientation, protein sensing, tag-free diagnosisNguyen TTHan GRJang CHJu HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 25-32 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Nguyen TT Han GR Jang CH Ju H Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
description	Tan Tai Nguyen,1 Gyeo-Re Han,2 Chang-Hyun Jang,1,2 Heongkyu Ju1,3,4 1Department of Bionano Technology, 2Department of Nano-Chemistry, 3Department of Nano-Physics, Gachon University, Seongnam-City, 4Neuroscience Institute, Gil Hospital, Incheon, South Korea Purpose: We present a polarization-sensitive optical detection platform for label-free quantitative optical biosensing diagnosis using liquid crystals (LCs). This is capable of determining quantitatively the optical birefringence of optical cells containing LCs, whose orientation depends on the immobilized biomolecules.Patients and methods: This technique uses a polarization-dependent double-port detection without any polarizer at a single wavelength and removes the need of aligning optical cells of LCs in the azimuthal direction, with respect to the light path through the optical cell. Thus, this technique enables a stand-alone detection in a relatively compact format without an additional optical instrument, such as a retardation compensator, a Michael–Levy chart, and a spectrophotometer, in order to determine the optical birefringence quantitatively.Results: We demonstrate that bovine serum albumin immobilized on the gold surface of the cell hybrid interfaces that support both homeotropic and planar anchoring of LCs causes optical phase retardation change which can be determined quantitatively. We also provide estimation of the zenithal orientation of LCs near the gold surface of the hybrid interfaces, based on the phase retardation determined. The estimated limit of bovine serum albumin detection is approximately 2.1 µM.Conclusion: This optical technique with LCs can serve an optical platform for label-free quantitative diagnosis of proteins in a real time manner. Keywords: biological sensors, LC orientation, protein sensing, tag-free diagnosis
format	article
author	Nguyen TT Han GR Jang CH Ju H
author_facet	Nguyen TT Han GR Jang CH Ju H
author_sort	Nguyen TT
title	Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
title_short	Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
title_full	Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
title_fullStr	Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
title_full_unstemmed	Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
title_sort	optical birefringence of liquid crystals for label-free optical biosensing diagnosis
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/5c9b30130d9b4cc0a3e9d2dd2766e66b
work_keys_str_mv	AT nguyentt opticalbirefringenceofliquidcrystalsforlabelfreeopticalbiosensingdiagnosis AT hangr opticalbirefringenceofliquidcrystalsforlabelfreeopticalbiosensingdiagnosis AT jangch opticalbirefringenceofliquidcrystalsforlabelfreeopticalbiosensingdiagnosis AT juh opticalbirefringenceofliquidcrystalsforlabelfreeopticalbiosensingdiagnosis
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Optical birefringence of liquid crystals for label-free optical biosensing diagnosis

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