Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.

The preterm gut microbiome is a complex dynamic community influenced by genetic and environmental factors and is implicated in the pathogenesis of necrotising enterocolitis (NEC) and sepsis. We aimed to explore the longitudinal development of the gut microbiome in preterm twins to determine how shar...

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Autores principales:	Christopher J Stewart, Emma C L Marrs, Andrew Nelson, Clare Lanyon, John D Perry, Nicholas D Embleton, Stephen P Cummings, Janet E Berrington
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ca37f97883b4bc2bdfc994ce3821d78
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spelling	oai:doaj.org-article:5ca37f97883b4bc2bdfc994ce3821d782021-11-18T08:57:32ZDevelopment of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.1932-620310.1371/journal.pone.0073465https://doaj.org/article/5ca37f97883b4bc2bdfc994ce3821d782013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023682/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The preterm gut microbiome is a complex dynamic community influenced by genetic and environmental factors and is implicated in the pathogenesis of necrotising enterocolitis (NEC) and sepsis. We aimed to explore the longitudinal development of the gut microbiome in preterm twins to determine how shared environmental and genetic factors may influence temporal changes and compared this to the expressed breast milk (EBM) microbiome. Stool samples (n = 173) from 27 infants (12 twin pairs and 1 triplet set) and EBM (n = 18) from 4 mothers were collected longitudinally. All samples underwent PCR-DGGE (denaturing gradient gel electrophoresis) analysis and a selected subset underwent 454 pyrosequencing. Stool and EBM shared a core microbiome dominated by Enterobacteriaceae, Enterococcaceae, and Staphylococcaceae. The gut microbiome showed greater similarity between siblings compared to unrelated individuals. Pyrosequencing revealed a reduction in diversity and increasing dominance of Escherichia sp. preceding NEC that was not observed in the healthy twin. Antibiotic treatment had a substantial effect on the gut microbiome, reducing Escherichia sp. and increasing other Enterobacteriaceae. This study demonstrates related preterm twins share similar gut microbiome development, even within the complex environment of neonatal intensive care. This is likely a result of shared genetic and immunomodulatory factors as well as exposure to the same maternal microbiome during birth, skin contact and exposure to EBM. Environmental factors including antibiotic exposure and feeding are additional significant determinants of community structure, regardless of host genetics.Christopher J StewartEmma C L MarrsAndrew NelsonClare LanyonJohn D PerryNicholas D EmbletonStephen P CummingsJanet E BerringtonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73465 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher J Stewart Emma C L Marrs Andrew Nelson Clare Lanyon John D Perry Nicholas D Embleton Stephen P Cummings Janet E Berrington Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
description	The preterm gut microbiome is a complex dynamic community influenced by genetic and environmental factors and is implicated in the pathogenesis of necrotising enterocolitis (NEC) and sepsis. We aimed to explore the longitudinal development of the gut microbiome in preterm twins to determine how shared environmental and genetic factors may influence temporal changes and compared this to the expressed breast milk (EBM) microbiome. Stool samples (n = 173) from 27 infants (12 twin pairs and 1 triplet set) and EBM (n = 18) from 4 mothers were collected longitudinally. All samples underwent PCR-DGGE (denaturing gradient gel electrophoresis) analysis and a selected subset underwent 454 pyrosequencing. Stool and EBM shared a core microbiome dominated by Enterobacteriaceae, Enterococcaceae, and Staphylococcaceae. The gut microbiome showed greater similarity between siblings compared to unrelated individuals. Pyrosequencing revealed a reduction in diversity and increasing dominance of Escherichia sp. preceding NEC that was not observed in the healthy twin. Antibiotic treatment had a substantial effect on the gut microbiome, reducing Escherichia sp. and increasing other Enterobacteriaceae. This study demonstrates related preterm twins share similar gut microbiome development, even within the complex environment of neonatal intensive care. This is likely a result of shared genetic and immunomodulatory factors as well as exposure to the same maternal microbiome during birth, skin contact and exposure to EBM. Environmental factors including antibiotic exposure and feeding are additional significant determinants of community structure, regardless of host genetics.
format	article
author	Christopher J Stewart Emma C L Marrs Andrew Nelson Clare Lanyon John D Perry Nicholas D Embleton Stephen P Cummings Janet E Berrington
author_facet	Christopher J Stewart Emma C L Marrs Andrew Nelson Clare Lanyon John D Perry Nicholas D Embleton Stephen P Cummings Janet E Berrington
author_sort	Christopher J Stewart
title	Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
title_short	Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
title_full	Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
title_fullStr	Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
title_full_unstemmed	Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
title_sort	development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5ca37f97883b4bc2bdfc994ce3821d78
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Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis.

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