CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs

Off-target editing remains a concern for therapeutic applications of CRISPR-Cas9. Here the authors present CRISPR GUARD, which uses very short non-cleaving gRNAs to prevent editing at off-target sites.

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Autores principales:	Matthew A. Coelho, Etienne De Braekeleer, Mike Firth, Michal Bista, Sebastian Lukasiak, Maria Emanuela Cuomo, Benjamin J. M. Taylor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5ca3e3c9d50c40859d3cc5b4b7665184
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spelling	oai:doaj.org-article:5ca3e3c9d50c40859d3cc5b4b76651842021-12-02T15:10:56ZCRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs10.1038/s41467-020-17952-52041-1723https://doaj.org/article/5ca3e3c9d50c40859d3cc5b4b76651842020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17952-5https://doaj.org/toc/2041-1723Off-target editing remains a concern for therapeutic applications of CRISPR-Cas9. Here the authors present CRISPR GUARD, which uses very short non-cleaving gRNAs to prevent editing at off-target sites.Matthew A. CoelhoEtienne De BraekeleerMike FirthMichal BistaSebastian LukasiakMaria Emanuela CuomoBenjamin J. M. TaylorNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Matthew A. Coelho Etienne De Braekeleer Mike Firth Michal Bista Sebastian Lukasiak Maria Emanuela Cuomo Benjamin J. M. Taylor CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
description	Off-target editing remains a concern for therapeutic applications of CRISPR-Cas9. Here the authors present CRISPR GUARD, which uses very short non-cleaving gRNAs to prevent editing at off-target sites.
format	article
author	Matthew A. Coelho Etienne De Braekeleer Mike Firth Michal Bista Sebastian Lukasiak Maria Emanuela Cuomo Benjamin J. M. Taylor
author_facet	Matthew A. Coelho Etienne De Braekeleer Mike Firth Michal Bista Sebastian Lukasiak Maria Emanuela Cuomo Benjamin J. M. Taylor
author_sort	Matthew A. Coelho
title	CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
title_short	CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
title_full	CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
title_fullStr	CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
title_full_unstemmed	CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
title_sort	crispr guard protects off-target sites from cas9 nuclease activity using short guide rnas
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5ca3e3c9d50c40859d3cc5b4b7665184
work_keys_str_mv	AT matthewacoelho crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas AT etiennedebraekeleer crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas AT mikefirth crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas AT michalbista crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas AT sebastianlukasiak crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas AT mariaemanuelacuomo crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas AT benjaminjmtaylor crisprguardprotectsofftargetsitesfromcas9nucleaseactivityusingshortguidernas
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CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs

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