Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders

BACKGROUND AND OBJECTIVE: Fungal agents, especially Candida species, can cause fungal and life-threatening lung infections in children. The present study was performed to identify Candida isolates obtained from bronchoalveolar lavage (BAL) specimens of children admitted to Akbar Children's Hosp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E Kashefi, SJ Seyedi, H Zarrinfar, A Fata, H Mehrad-Majd, MJ Najafzadeh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2021
Materias:	candida children bal pulmonary infection. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5ca47a22d2bf4dcaabb1ce966c0f3e09
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5ca47a22d2bf4dcaabb1ce966c0f3e09
record_format	dspace
spelling	oai:doaj.org-article:5ca47a22d2bf4dcaabb1ce966c0f3e092021-11-29T07:40:49ZMolecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders1561-41072251-7170https://doaj.org/article/5ca47a22d2bf4dcaabb1ce966c0f3e092021-03-01T00:00:00Zhttp://jbums.org/article-1-9749-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Fungal agents, especially Candida species, can cause fungal and life-threatening lung infections in children. The present study was performed to identify Candida isolates obtained from bronchoalveolar lavage (BAL) specimens of children admitted to Akbar Children's Hospital in Mashhad. METHODS: In this cross-sectional study, 210 BAL specimens of hospitalized children with pulmonary disorders during 27 months (April 2018 to June 2020) were examined. Routine mycology tests including direct examination with 20% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar containing chloramphenicol were performed for these specimens. The genomic DNA of Candida colonies were amplified by Multiplex PCR, and Candida species were identified based on the electrophoresis pattern. FINDINGS: The patients included 90 females (42.9%) and 120 females (57.1%) and their age range was between 2 months to 16 years and their mean age was 4.6±3.16 years. Among clinical specimens, 20 specimens (9.5%) had direct microscopic results and positive culture for Candida. Identified Candida isolates included C. albicans in 15 cases (75%), C. dubliniensis in 2 cases (10%), C. parapsilosis in 2 cases (10%) and C. tropicalis in 1 case (5%). Among the underlying factors, corticosteroid receptors and patients with neutropenia had the highest frequency (73 patients, 34.8%). CONCLUSION: The results of the study showed that C. albicans was the most common Candida isolate in the BAL specimen of hospitalized children with pulmonary disorders. Children under 4 years of age were the most affected group and pneumonia was the most common clinical form in patients with Candida infection.E KashefiSJ SeyediH ZarrinfarA FataH Mehrad-MajdMJ NajafzadehBabol University of Medical Sciencesarticlecandidachildrenbalpulmonaryinfection.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 331-336 (2021)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	candida children bal pulmonary infection. Medicine R Medicine (General) R5-920
spellingShingle	candida children bal pulmonary infection. Medicine R Medicine (General) R5-920 E Kashefi SJ Seyedi H Zarrinfar A Fata H Mehrad-Majd MJ Najafzadeh Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders
description	BACKGROUND AND OBJECTIVE: Fungal agents, especially Candida species, can cause fungal and life-threatening lung infections in children. The present study was performed to identify Candida isolates obtained from bronchoalveolar lavage (BAL) specimens of children admitted to Akbar Children's Hospital in Mashhad. METHODS: In this cross-sectional study, 210 BAL specimens of hospitalized children with pulmonary disorders during 27 months (April 2018 to June 2020) were examined. Routine mycology tests including direct examination with 20% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar containing chloramphenicol were performed for these specimens. The genomic DNA of Candida colonies were amplified by Multiplex PCR, and Candida species were identified based on the electrophoresis pattern. FINDINGS: The patients included 90 females (42.9%) and 120 females (57.1%) and their age range was between 2 months to 16 years and their mean age was 4.6±3.16 years. Among clinical specimens, 20 specimens (9.5%) had direct microscopic results and positive culture for Candida. Identified Candida isolates included C. albicans in 15 cases (75%), C. dubliniensis in 2 cases (10%), C. parapsilosis in 2 cases (10%) and C. tropicalis in 1 case (5%). Among the underlying factors, corticosteroid receptors and patients with neutropenia had the highest frequency (73 patients, 34.8%). CONCLUSION: The results of the study showed that C. albicans was the most common Candida isolate in the BAL specimen of hospitalized children with pulmonary disorders. Children under 4 years of age were the most affected group and pneumonia was the most common clinical form in patients with Candida infection.
format	article
author	E Kashefi SJ Seyedi H Zarrinfar A Fata H Mehrad-Majd MJ Najafzadeh
author_facet	E Kashefi SJ Seyedi H Zarrinfar A Fata H Mehrad-Majd MJ Najafzadeh
author_sort	E Kashefi
title	Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders
title_short	Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders
title_full	Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders
title_fullStr	Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders
title_full_unstemmed	Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders
title_sort	molecular identification of candida species in bronchoalveolar lavage specimens of hospitalized children with pulmonary disorders
publisher	Babol University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/5ca47a22d2bf4dcaabb1ce966c0f3e09
work_keys_str_mv	AT ekashefi molecularidentificationofcandidaspeciesinbronchoalveolarlavagespecimensofhospitalizedchildrenwithpulmonarydisorders AT sjseyedi molecularidentificationofcandidaspeciesinbronchoalveolarlavagespecimensofhospitalizedchildrenwithpulmonarydisorders AT hzarrinfar molecularidentificationofcandidaspeciesinbronchoalveolarlavagespecimensofhospitalizedchildrenwithpulmonarydisorders AT afata molecularidentificationofcandidaspeciesinbronchoalveolarlavagespecimensofhospitalizedchildrenwithpulmonarydisorders AT hmehradmajd molecularidentificationofcandidaspeciesinbronchoalveolarlavagespecimensofhospitalizedchildrenwithpulmonarydisorders AT mjnajafzadeh molecularidentificationofcandidaspeciesinbronchoalveolarlavagespecimensofhospitalizedchildrenwithpulmonarydisorders
_version_	1718407525293883392

Molecular Identification of Candida Species in Bronchoalveolar Lavage Specimens of Hospitalized Children with Pulmonary Disorders

Ejemplares similares