Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma

Oral squamous cell carcinoma (OSCC) is one of the most common types of malignant tumor. Sequestosome 1 (SQSTM1) serves as an adaptor of autophagy for degrading protein aggregates. The regulation of autophagy by EGFR and its clinical impacts are indicated in various types of cancer. However, the asso...

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Autores principales:	Yu-Kai Tseng, Chun-Feng Chen, Chih-Wen Shu, Cheng-Hsin Lee, Yan-Ting Chou, Yi-Jing Li, Huei-Han Liou, Jiin-Tsuey Cheng, Chun-Lin Chen, Luo-Ping Ger, Pei-Feng Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	oral squamous cell carcinoma sequestosome 1 epidermal growth factor receptor malignancy prognosis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/5ca87154c7174028ac89c392c7f9be16
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spelling	oai:doaj.org-article:5ca87154c7174028ac89c392c7f9be162021-11-25T17:54:30ZEffect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma10.3390/ijms2222122261422-00671661-6596https://doaj.org/article/5ca87154c7174028ac89c392c7f9be162021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12226https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Oral squamous cell carcinoma (OSCC) is one of the most common types of malignant tumor. Sequestosome 1 (SQSTM1) serves as an adaptor of autophagy for degrading protein aggregates. The regulation of autophagy by EGFR and its clinical impacts are indicated in various types of cancer. However, the association of EGFR and SQSTM1 in OSCC is still unknown. Our results show that the expression levels of SQSTM1 and EGFR proteins are higher in tumor tissues than in the corresponding tumor-adjacent (CTAN) tissues of OSCC patients. The expression levels of SQSTM1 were positively associated with the EGFR expression level. High co-expression of SQSTM1 and EGFR is associated with poor prognosis in OSCC patients. Moreover, SQSTM1 expression is decreased in EGFR-knockdown cells. Cell growth and invasion/migration are also decreased in cells with single/combined knockdowns of EGFR and SQSTM1 or in SQSTM1-knockdown cells without EGFR kinase inhibitor Lapatinib treatment compared to that in scrambled cells. However, cell growth and invasion/metastasis were not significantly different between the scrambled cells and SQSTM1-knockdown cells in the presence of Lapatinib. This study is the first to indicate the biological roles and clinical significance of SQSTM1 regulation by EGFR in OSCC.Yu-Kai TsengChun-Feng ChenChih-Wen ShuCheng-Hsin LeeYan-Ting ChouYi-Jing LiHuei-Han LiouJiin-Tsuey ChengChun-Lin ChenLuo-Ping GerPei-Feng LiuMDPI AGarticleoral squamous cell carcinomasequestosome 1epidermal growth factor receptormalignancyprognosisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12226, p 12226 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oral squamous cell carcinoma sequestosome 1 epidermal growth factor receptor malignancy prognosis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	oral squamous cell carcinoma sequestosome 1 epidermal growth factor receptor malignancy prognosis Biology (General) QH301-705.5 Chemistry QD1-999 Yu-Kai Tseng Chun-Feng Chen Chih-Wen Shu Cheng-Hsin Lee Yan-Ting Chou Yi-Jing Li Huei-Han Liou Jiin-Tsuey Cheng Chun-Lin Chen Luo-Ping Ger Pei-Feng Liu Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma
description	Oral squamous cell carcinoma (OSCC) is one of the most common types of malignant tumor. Sequestosome 1 (SQSTM1) serves as an adaptor of autophagy for degrading protein aggregates. The regulation of autophagy by EGFR and its clinical impacts are indicated in various types of cancer. However, the association of EGFR and SQSTM1 in OSCC is still unknown. Our results show that the expression levels of SQSTM1 and EGFR proteins are higher in tumor tissues than in the corresponding tumor-adjacent (CTAN) tissues of OSCC patients. The expression levels of SQSTM1 were positively associated with the EGFR expression level. High co-expression of SQSTM1 and EGFR is associated with poor prognosis in OSCC patients. Moreover, SQSTM1 expression is decreased in EGFR-knockdown cells. Cell growth and invasion/migration are also decreased in cells with single/combined knockdowns of EGFR and SQSTM1 or in SQSTM1-knockdown cells without EGFR kinase inhibitor Lapatinib treatment compared to that in scrambled cells. However, cell growth and invasion/metastasis were not significantly different between the scrambled cells and SQSTM1-knockdown cells in the presence of Lapatinib. This study is the first to indicate the biological roles and clinical significance of SQSTM1 regulation by EGFR in OSCC.
format	article
author	Yu-Kai Tseng Chun-Feng Chen Chih-Wen Shu Cheng-Hsin Lee Yan-Ting Chou Yi-Jing Li Huei-Han Liou Jiin-Tsuey Cheng Chun-Lin Chen Luo-Ping Ger Pei-Feng Liu
author_facet	Yu-Kai Tseng Chun-Feng Chen Chih-Wen Shu Cheng-Hsin Lee Yan-Ting Chou Yi-Jing Li Huei-Han Liou Jiin-Tsuey Cheng Chun-Lin Chen Luo-Ping Ger Pei-Feng Liu
author_sort	Yu-Kai Tseng
title	Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma
title_short	Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma
title_full	Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma
title_fullStr	Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma
title_full_unstemmed	Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma
title_sort	effect of egfr on sqstm1 expression in malignancy and tumor progression of oral squamous cell carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5ca87154c7174028ac89c392c7f9be16
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Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma

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