Raman spectroscopy on blood serum samples of patients with end-stage liver disease.

Raman spectroscopy has shown to be a promising method for the examination of biomedical samples. However, until now, its efficacy has not been established in clinical diagnostics. In this study, Raman spectroscopy's potential application in medical laboratories is evaluated for a large variety...

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Autores principales:	René Staritzbichler, Pascal Hunold, Irina Estrela-Lopis, Peter Werner Hildebrand, Berend Isermann, Thorsten Kaiser
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cad61701f074d709098ba14c62d7639
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spelling	oai:doaj.org-article:5cad61701f074d709098ba14c62d76392021-12-02T20:04:43ZRaman spectroscopy on blood serum samples of patients with end-stage liver disease.1932-620310.1371/journal.pone.0256045https://doaj.org/article/5cad61701f074d709098ba14c62d76392021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256045https://doaj.org/toc/1932-6203Raman spectroscopy has shown to be a promising method for the examination of biomedical samples. However, until now, its efficacy has not been established in clinical diagnostics. In this study, Raman spectroscopy's potential application in medical laboratories is evaluated for a large variety (38) of biomarkers. Given 234 serum samples from a cohort of patients with different stages of liver disease, we performed Raman spectroscopy at 780nm excitation wavelength. The Raman spectra were analyzed in combination with the results of routine diagnostics using specifically developed complex mathematical algorithms, including fluorescence filtering, frequency subset selection and several overfitting circumventing strategies, such as independent validation. With the results of this cohort, which were validated in 328 independent samples, a significant proof-of-concept study was completed. This study highlights the need to prevent overfitting and to use independent data for validation. The results reveal that Raman spectroscopy has high potential for use in medical laboratory diagnostics to simultaneously quantify multiple biomarkers.René StaritzbichlerPascal HunoldIrina Estrela-LopisPeter Werner HildebrandBerend IsermannThorsten KaiserPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256045 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q René Staritzbichler Pascal Hunold Irina Estrela-Lopis Peter Werner Hildebrand Berend Isermann Thorsten Kaiser Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
description	Raman spectroscopy has shown to be a promising method for the examination of biomedical samples. However, until now, its efficacy has not been established in clinical diagnostics. In this study, Raman spectroscopy's potential application in medical laboratories is evaluated for a large variety (38) of biomarkers. Given 234 serum samples from a cohort of patients with different stages of liver disease, we performed Raman spectroscopy at 780nm excitation wavelength. The Raman spectra were analyzed in combination with the results of routine diagnostics using specifically developed complex mathematical algorithms, including fluorescence filtering, frequency subset selection and several overfitting circumventing strategies, such as independent validation. With the results of this cohort, which were validated in 328 independent samples, a significant proof-of-concept study was completed. This study highlights the need to prevent overfitting and to use independent data for validation. The results reveal that Raman spectroscopy has high potential for use in medical laboratory diagnostics to simultaneously quantify multiple biomarkers.
format	article
author	René Staritzbichler Pascal Hunold Irina Estrela-Lopis Peter Werner Hildebrand Berend Isermann Thorsten Kaiser
author_facet	René Staritzbichler Pascal Hunold Irina Estrela-Lopis Peter Werner Hildebrand Berend Isermann Thorsten Kaiser
author_sort	René Staritzbichler
title	Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
title_short	Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
title_full	Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
title_fullStr	Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
title_full_unstemmed	Raman spectroscopy on blood serum samples of patients with end-stage liver disease.
title_sort	raman spectroscopy on blood serum samples of patients with end-stage liver disease.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5cad61701f074d709098ba14c62d7639
work_keys_str_mv	AT renestaritzbichler ramanspectroscopyonbloodserumsamplesofpatientswithendstageliverdisease AT pascalhunold ramanspectroscopyonbloodserumsamplesofpatientswithendstageliverdisease AT irinaestrelalopis ramanspectroscopyonbloodserumsamplesofpatientswithendstageliverdisease AT peterwernerhildebrand ramanspectroscopyonbloodserumsamplesofpatientswithendstageliverdisease AT berendisermann ramanspectroscopyonbloodserumsamplesofpatientswithendstageliverdisease AT thorstenkaiser ramanspectroscopyonbloodserumsamplesofpatientswithendstageliverdisease
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Raman spectroscopy on blood serum samples of patients with end-stage liver disease.

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