Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue

The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fibrin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was car...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Horňák Slavomír, Harvanová Denisa, Ledecký Valent, Hluchý Marian, Valenčáková-Agyagosová Alexandra, Amrichová Judita, Rosocha Ján, Vaško Gabriel, Švihla Róbert, Petrovič Vladimír
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	rabbits articular cartilage defect chondrocyte implants collagen hyaluronan Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5cb2c9522eaa40e8a5c505ee1d476eed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5cb2c9522eaa40e8a5c505ee1d476eed
record_format	dspace
spelling	oai:doaj.org-article:5cb2c9522eaa40e8a5c505ee1d476eed2021-11-17T21:27:52ZReparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue1820-744810.2478/acve-2014-0007https://doaj.org/article/5cb2c9522eaa40e8a5c505ee1d476eed2014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0007https://doaj.org/toc/1820-7448The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fibrin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was carried out on 15 domestic rabbits randomly assigned to five groups (n = 3 in each) with different treatments of artificially created chondral defects (ChD´s). These defects were made in a non-load-bearing area of medial condyle of the distal femur, and were treated as follows: 1st and 3rd group: the ChD´s were filled with autologous or allogenous chondrocytes seeded on Col type I-HYA scaffolds, respectively. The scaffolds were fixed to the ChD´s by fibrin glue Beriplast®; 2nd and 4th group: the ChD´s were filled with a suspension of autologous or allogenous chondrocytes in fibrin glue Beriplast®, respectively, and they were immediately covered by unseeded Col type I-HYA scaffolds; Control group: the ChD´s were left to heal spontaneously without any treatment. Macroscopical, histological and immunohistochemical analyses of the ChD´s were performed 12 months after the treatment. In all treated groups, the chondrocytes were capable to proliferate and produce the cartilage extracellular matrix, including proteoglycans and type II collagen, as compared to the control “untreated” group. On the other hand, the production of hyaline-like cartilage tissue confirmed that both therapeutic methods using autologous chondrocytes can be applied successfully for the treatment of chondral defects in rabbits.Horňák SlavomírHarvanová DenisaLedecký ValentHluchý MarianValenčáková-Agyagosová AlexandraAmrichová JuditaRosocha JánVaško GabrielŠvihla RóbertPetrovič VladimírSciendoarticlerabbitsarticular cartilage defectchondrocyte implantscollagenhyaluronanVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 61-72 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	rabbits articular cartilage defect chondrocyte implants collagen hyaluronan Veterinary medicine SF600-1100
spellingShingle	rabbits articular cartilage defect chondrocyte implants collagen hyaluronan Veterinary medicine SF600-1100 Horňák Slavomír Harvanová Denisa Ledecký Valent Hluchý Marian Valenčáková-Agyagosová Alexandra Amrichová Judita Rosocha Ján Vaško Gabriel Švihla Róbert Petrovič Vladimír Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
description	The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fibrin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was carried out on 15 domestic rabbits randomly assigned to five groups (n = 3 in each) with different treatments of artificially created chondral defects (ChD´s). These defects were made in a non-load-bearing area of medial condyle of the distal femur, and were treated as follows: 1st and 3rd group: the ChD´s were filled with autologous or allogenous chondrocytes seeded on Col type I-HYA scaffolds, respectively. The scaffolds were fixed to the ChD´s by fibrin glue Beriplast®; 2nd and 4th group: the ChD´s were filled with a suspension of autologous or allogenous chondrocytes in fibrin glue Beriplast®, respectively, and they were immediately covered by unseeded Col type I-HYA scaffolds; Control group: the ChD´s were left to heal spontaneously without any treatment. Macroscopical, histological and immunohistochemical analyses of the ChD´s were performed 12 months after the treatment. In all treated groups, the chondrocytes were capable to proliferate and produce the cartilage extracellular matrix, including proteoglycans and type II collagen, as compared to the control “untreated” group. On the other hand, the production of hyaline-like cartilage tissue confirmed that both therapeutic methods using autologous chondrocytes can be applied successfully for the treatment of chondral defects in rabbits.
format	article
author	Horňák Slavomír Harvanová Denisa Ledecký Valent Hluchý Marian Valenčáková-Agyagosová Alexandra Amrichová Judita Rosocha Ján Vaško Gabriel Švihla Róbert Petrovič Vladimír
author_facet	Horňák Slavomír Harvanová Denisa Ledecký Valent Hluchý Marian Valenčáková-Agyagosová Alexandra Amrichová Judita Rosocha Ján Vaško Gabriel Švihla Róbert Petrovič Vladimír
author_sort	Horňák Slavomír
title	Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
title_short	Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
title_full	Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
title_fullStr	Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
title_full_unstemmed	Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
title_sort	reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/5cb2c9522eaa40e8a5c505ee1d476eed
work_keys_str_mv	AT hornakslavomir reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT harvanovadenisa reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT ledeckyvalent reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT hluchymarian reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT valencakovaagyagosovaalexandra reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT amrichovajudita reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT rosochajan reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT vaskogabriel reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT svihlarobert reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue AT petrovicvladimir reparationofchondraldefectsinrabbitsbyautologousandallogenouschondrocytesseededoncollagenhyaluronanscaffoldorsuspendedinfibringlue
_version_	1718425284073488384

Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue

Ejemplares similares