Silkworm coatomers and their role in tube expansion of posterior silkgland.

<h4>Background</h4>Coat protein complex I (COPI) vesicles, coated by seven coatomer subunits, are mainly responsible for Golgi-to-ER transport. Silkworm posterior silkgland (PSG), a highly differentiated secretory tissue, secretes fibroin for silk production, but many physiological proce...

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Autores principales:	Qiao Wang, Birong Shen, Pengli Zheng, Hui Feng, Liang Chen, Jing Zhang, Chuanxi Zhang, Guozheng Zhang, Junlin Teng, Jianguo Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cb7bf72d2d94deb9e8d4b9a46306dde
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spelling	oai:doaj.org-article:5cb7bf72d2d94deb9e8d4b9a46306dde2021-11-18T07:03:27ZSilkworm coatomers and their role in tube expansion of posterior silkgland.1932-620310.1371/journal.pone.0013252https://doaj.org/article/5cb7bf72d2d94deb9e8d4b9a46306dde2010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20967265/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Coat protein complex I (COPI) vesicles, coated by seven coatomer subunits, are mainly responsible for Golgi-to-ER transport. Silkworm posterior silkgland (PSG), a highly differentiated secretory tissue, secretes fibroin for silk production, but many physiological processes in the PSG cells await further investigation.<h4>Methodology/principal findings</h4>Here, to investigate the role of silkworm COPI, we cloned six silkworm COPI subunits (α, β, β', δ, ε, and ζ-COP), determined their peak expression in day 2 in fifth-instar PSG, and visualized the localization of COPI, as a coat complex, with cis-Golgi. By dsRNA injection into silkworm larvae, we suppressed the expression of α-, β'- and γ-COP, and demonstrated that COPI subunits were required for PSG tube expansion. Knockdown of α-COP disrupted the integrity of Golgi apparatus and led to a narrower glandular lumen of the PSG, suggesting that silkworm COPI is essential for PSG tube expansion.<h4>Conclusions/significance</h4>The initial characterization reveals the essential roles of silkworm COPI in PSG. Although silkworm COPI resembles the previously characterized coatomers in other organisms, some surprising findings require further investigation. Therefore, our results suggest the silkworm as a model for studying intracellular transport, and would facilitate the establishment of silkworm PSG as an efficient bioreactor.Qiao WangBirong ShenPengli ZhengHui FengLiang ChenJing ZhangChuanxi ZhangGuozheng ZhangJunlin TengJianguo ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13252 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiao Wang Birong Shen Pengli Zheng Hui Feng Liang Chen Jing Zhang Chuanxi Zhang Guozheng Zhang Junlin Teng Jianguo Chen Silkworm coatomers and their role in tube expansion of posterior silkgland.
description	<h4>Background</h4>Coat protein complex I (COPI) vesicles, coated by seven coatomer subunits, are mainly responsible for Golgi-to-ER transport. Silkworm posterior silkgland (PSG), a highly differentiated secretory tissue, secretes fibroin for silk production, but many physiological processes in the PSG cells await further investigation.<h4>Methodology/principal findings</h4>Here, to investigate the role of silkworm COPI, we cloned six silkworm COPI subunits (α, β, β', δ, ε, and ζ-COP), determined their peak expression in day 2 in fifth-instar PSG, and visualized the localization of COPI, as a coat complex, with cis-Golgi. By dsRNA injection into silkworm larvae, we suppressed the expression of α-, β'- and γ-COP, and demonstrated that COPI subunits were required for PSG tube expansion. Knockdown of α-COP disrupted the integrity of Golgi apparatus and led to a narrower glandular lumen of the PSG, suggesting that silkworm COPI is essential for PSG tube expansion.<h4>Conclusions/significance</h4>The initial characterization reveals the essential roles of silkworm COPI in PSG. Although silkworm COPI resembles the previously characterized coatomers in other organisms, some surprising findings require further investigation. Therefore, our results suggest the silkworm as a model for studying intracellular transport, and would facilitate the establishment of silkworm PSG as an efficient bioreactor.
format	article
author	Qiao Wang Birong Shen Pengli Zheng Hui Feng Liang Chen Jing Zhang Chuanxi Zhang Guozheng Zhang Junlin Teng Jianguo Chen
author_facet	Qiao Wang Birong Shen Pengli Zheng Hui Feng Liang Chen Jing Zhang Chuanxi Zhang Guozheng Zhang Junlin Teng Jianguo Chen
author_sort	Qiao Wang
title	Silkworm coatomers and their role in tube expansion of posterior silkgland.
title_short	Silkworm coatomers and their role in tube expansion of posterior silkgland.
title_full	Silkworm coatomers and their role in tube expansion of posterior silkgland.
title_fullStr	Silkworm coatomers and their role in tube expansion of posterior silkgland.
title_full_unstemmed	Silkworm coatomers and their role in tube expansion of posterior silkgland.
title_sort	silkworm coatomers and their role in tube expansion of posterior silkgland.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/5cb7bf72d2d94deb9e8d4b9a46306dde
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_version_	1718423978409721856

Silkworm coatomers and their role in tube expansion of posterior silkgland.

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