Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital

Objective: To examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in Citrobacter species isolated from hospitalized patients in a tertiary care hospital. Methods: A prospective study was conducted in a 1000-bed tertiary care center in Pune, India from October...

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Autores principales:	Ashok Kumar Praharaj, Atul Khajuria, Mahadevan Kumar, Naveen Grover
Formato:	article
Lenguaje:	EN
Publicado:	Thieme Medical and Scientific Publishers Pvt. Ltd. 2016
Materias:	blandm-1 blavim-2 combined-disc synergy test citrobacter freundii citrobacter koseri double-disc synergy tests metallo-beta-lactamase modified hodge test Medicine R
Acceso en línea:	https://doaj.org/article/5cb9c41ff3f94d389be74372f8b0f0b0
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spelling	oai:doaj.org-article:5cb9c41ff3f94d389be74372f8b0f0b02021-12-02T16:34:20ZPhenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital2231-07702249-446410.4103/2231-0770.173578https://doaj.org/article/5cb9c41ff3f94d389be74372f8b0f0b02016-01-01T00:00:00Zhttp://www.thieme-connect.de/DOI/DOI?10.4103/2231-0770.173578https://doaj.org/toc/2231-0770https://doaj.org/toc/2249-4464Objective: To examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in Citrobacter species isolated from hospitalized patients in a tertiary care hospital. Methods: A prospective study was conducted in a 1000-bed tertiary care center in Pune, India from October 2010 to October 2013. A total of 221 Citrobacter spp. isolates were recovered from clinical specimens from different patients (one isolate per patient) admitted to the surgical ward, medical ward and medical and surgical Intensive Care Units. Polymerase chain reaction (PCR) assays and sequencing were used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine their transferability. Isolate relatedness were determined by repetitive element based-PCR, enterobacterial repetitive intergenic consensus-PCR and randomly amplified polymorphic DNA. Results: Among 221 tested isolates of Citrobacter spp. recovered from various clinical specimens, 179 (80.9%) isolates showed minimum inhibitory concentration (MIC) >4 μg/ml against meropenem and imipenem. One hundred and forty-five isolates with increased MICs value against carbapenems were further processed for molecular characterization of beta-lactamase genes. Susceptibility profiling of the isolates indicated that 100% retained susceptibility to colistin. Conjugation experiments indicated that blaNDM-1was transferable via a plasmid. Conclusion: The ease of NDM-1 plasmid transmissibility may help their dissemination among the Citrobacter species as well as to others in Enterobacteriaceae. Early detection, antimicrobial stewardship and adequate infection control measures will help in limiting the spread of these organisms.Ashok Kumar PraharajAtul KhajuriaMahadevan KumarNaveen GroverThieme Medical and Scientific Publishers Pvt. Ltd.articleblandm-1blavim-2combined-disc synergy testcitrobacter freundiicitrobacter koseridouble-disc synergy testsmetallo-beta-lactamasemodified hodge testMedicineRENAvicenna Journal of Medicine, Vol 06, Iss 01, Pp 17-27 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	blandm-1 blavim-2 combined-disc synergy test citrobacter freundii citrobacter koseri double-disc synergy tests metallo-beta-lactamase modified hodge test Medicine R
spellingShingle	blandm-1 blavim-2 combined-disc synergy test citrobacter freundii citrobacter koseri double-disc synergy tests metallo-beta-lactamase modified hodge test Medicine R Ashok Kumar Praharaj Atul Khajuria Mahadevan Kumar Naveen Grover Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital
description	Objective: To examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in Citrobacter species isolated from hospitalized patients in a tertiary care hospital. Methods: A prospective study was conducted in a 1000-bed tertiary care center in Pune, India from October 2010 to October 2013. A total of 221 Citrobacter spp. isolates were recovered from clinical specimens from different patients (one isolate per patient) admitted to the surgical ward, medical ward and medical and surgical Intensive Care Units. Polymerase chain reaction (PCR) assays and sequencing were used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine their transferability. Isolate relatedness were determined by repetitive element based-PCR, enterobacterial repetitive intergenic consensus-PCR and randomly amplified polymorphic DNA. Results: Among 221 tested isolates of Citrobacter spp. recovered from various clinical specimens, 179 (80.9%) isolates showed minimum inhibitory concentration (MIC) >4 μg/ml against meropenem and imipenem. One hundred and forty-five isolates with increased MICs value against carbapenems were further processed for molecular characterization of beta-lactamase genes. Susceptibility profiling of the isolates indicated that 100% retained susceptibility to colistin. Conjugation experiments indicated that blaNDM-1was transferable via a plasmid. Conclusion: The ease of NDM-1 plasmid transmissibility may help their dissemination among the Citrobacter species as well as to others in Enterobacteriaceae. Early detection, antimicrobial stewardship and adequate infection control measures will help in limiting the spread of these organisms.
format	article
author	Ashok Kumar Praharaj Atul Khajuria Mahadevan Kumar Naveen Grover
author_facet	Ashok Kumar Praharaj Atul Khajuria Mahadevan Kumar Naveen Grover
author_sort	Ashok Kumar Praharaj
title	Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital
title_short	Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital
title_full	Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital
title_fullStr	Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital
title_full_unstemmed	Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital
title_sort	phenotypic detection and molecular characterization of beta-lactamase genes among citrobacter species in a tertiary care hospital
publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
publishDate	2016
url	https://doaj.org/article/5cb9c41ff3f94d389be74372f8b0f0b0
work_keys_str_mv	AT ashokkumarpraharaj phenotypicdetectionandmolecularcharacterizationofbetalactamasegenesamongcitrobacterspeciesinatertiarycarehospital AT atulkhajuria phenotypicdetectionandmolecularcharacterizationofbetalactamasegenesamongcitrobacterspeciesinatertiarycarehospital AT mahadevankumar phenotypicdetectionandmolecularcharacterizationofbetalactamasegenesamongcitrobacterspeciesinatertiarycarehospital AT naveengrover phenotypicdetectionandmolecularcharacterizationofbetalactamasegenesamongcitrobacterspeciesinatertiarycarehospital
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Phenotypic detection and molecular characterization of beta-lactamase genes among Citrobacter species in a tertiary care hospital

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