Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma

Background As a new class of non-coding RNAs, circRNAs have been recently reported to be involved in the tumorigenesis and progression of human cancers. In the current study, we attempted to explore the potential function of a novel circRNA (hsa_circ_0013290) in hepatocellular carcinoma (HCC). Metho...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xi Luo, Yicun Liu, Han Li, Tiaochun Cheng, Jianjun Wu, Lin Chen, Linling Ju, Weihua Cai, Zhaolian Bian
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/5cbbb94ea520453aafa13290f6092b04
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5cbbb94ea520453aafa13290f6092b04
record_format	dspace
spelling	oai:doaj.org-article:5cbbb94ea520453aafa13290f6092b042021-11-21T01:34:43ZHsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma1073-274810.1177/10732748211055681https://doaj.org/article/5cbbb94ea520453aafa13290f6092b042021-11-01T00:00:00Zhttps://doi.org/10.1177/10732748211055681https://doaj.org/toc/1073-2748Background As a new class of non-coding RNAs, circRNAs have been recently reported to be involved in the tumorigenesis and progression of human cancers. In the current study, we attempted to explore the potential function of a novel circRNA (hsa_circ_0013290) in hepatocellular carcinoma (HCC). Methods Relative hsa_circ_0013290 expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The subcellular location of hsa_circ_0013290 was performed by RNA subcellular isolation and fluorescence in situ hybridization (FISH) assays. The effect of hsa_circ_0013290 on proliferation was detected by Cell Counting Kit-8 (CCK-8) assays. The effect of hsa_circ_0013290 on cell cycle distribution and apoptosis was detected by flow cytometry. The invasion and migration abilities of hsa_circ_0013290 were detected by transwell assays. Results Hsa_circ_0013290 is significantly upregulated in HCC cell lines and mainly located in cytoplasm of HCC cells. Hsa_circ_0013290 overexpression promotes cell invasion and migration and inhibits cell apoptosis. In contrast, hsa_circ_0013290 knockdown impedes cell invasion and migration and accelerates cell apoptosis. However, hsa_circ_0013290 did not affect cell proliferation. Conclusions Hsa_circ_0013290 is overexpressed in HCC cell lines and is mainly located in the cytoplasm of HCC cells. Hsa_circ_0013290 promotes cell invasion and migration, and inhibits cell apoptosis.Xi LuoYicun LiuHan LiTiaochun ChengJianjun WuLin ChenLinling JuWeihua CaiZhaolian BianSAGE PublishingarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Control, Vol 28 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Xi Luo Yicun Liu Han Li Tiaochun Cheng Jianjun Wu Lin Chen Linling Ju Weihua Cai Zhaolian Bian Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma
description	Background As a new class of non-coding RNAs, circRNAs have been recently reported to be involved in the tumorigenesis and progression of human cancers. In the current study, we attempted to explore the potential function of a novel circRNA (hsa_circ_0013290) in hepatocellular carcinoma (HCC). Methods Relative hsa_circ_0013290 expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The subcellular location of hsa_circ_0013290 was performed by RNA subcellular isolation and fluorescence in situ hybridization (FISH) assays. The effect of hsa_circ_0013290 on proliferation was detected by Cell Counting Kit-8 (CCK-8) assays. The effect of hsa_circ_0013290 on cell cycle distribution and apoptosis was detected by flow cytometry. The invasion and migration abilities of hsa_circ_0013290 were detected by transwell assays. Results Hsa_circ_0013290 is significantly upregulated in HCC cell lines and mainly located in cytoplasm of HCC cells. Hsa_circ_0013290 overexpression promotes cell invasion and migration and inhibits cell apoptosis. In contrast, hsa_circ_0013290 knockdown impedes cell invasion and migration and accelerates cell apoptosis. However, hsa_circ_0013290 did not affect cell proliferation. Conclusions Hsa_circ_0013290 is overexpressed in HCC cell lines and is mainly located in the cytoplasm of HCC cells. Hsa_circ_0013290 promotes cell invasion and migration, and inhibits cell apoptosis.
format	article
author	Xi Luo Yicun Liu Han Li Tiaochun Cheng Jianjun Wu Lin Chen Linling Ju Weihua Cai Zhaolian Bian
author_facet	Xi Luo Yicun Liu Han Li Tiaochun Cheng Jianjun Wu Lin Chen Linling Ju Weihua Cai Zhaolian Bian
author_sort	Xi Luo
title	Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma
title_short	Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma
title_full	Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma
title_fullStr	Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma
title_full_unstemmed	Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma
title_sort	hsa_circ_0013290 acts as cancer-promoting gene in hepatocellular carcinoma
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/5cbbb94ea520453aafa13290f6092b04
work_keys_str_mv	AT xiluo hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT yicunliu hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT hanli hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT tiaochuncheng hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT jianjunwu hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT linchen hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT linlingju hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT weihuacai hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma AT zhaolianbian hsacirc0013290actsascancerpromotinggeneinhepatocellularcarcinoma
_version_	1718419365734383616

Hsa_circ_0013290 Acts as Cancer-Promoting Gene in Hepatocellular Carcinoma

Ejemplares similares