Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight...

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Autores principales:	Veljović Ljubiša, Knežević Aleksandra, Milić Nenad, Krnjaić Dejan, Miković Radoš, Zorić Andrea, Marković Maja, Milićević Vesna, Stamenković Miodrag, Stanojević Maja, Maksimović-Zorić Jelena, Petrović Tamaš, Nišavić Jakov
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	bovine parainfluenza virus type 3 virus isolation rt-pcr serbia Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5cc1fc8d8f8c4b3495cc9b1f1a9d2acd
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spelling	oai:doaj.org-article:5cc1fc8d8f8c4b3495cc9b1f1a9d2acd2021-11-17T21:27:51ZIsolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia1820-744810.1515/acve-2016-0044https://doaj.org/article/5cc1fc8d8f8c4b3495cc9b1f1a9d2acd2016-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0044https://doaj.org/toc/1820-7448The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.Veljović LjubišaKnežević AleksandraMilić NenadKrnjaić DejanMiković RadošZorić AndreaMarković MajaMilićević VesnaStamenković MiodragStanojević MajaMaksimović-Zorić JelenaPetrović TamašNišavić JakovSciendoarticlebovine parainfluenza virus type 3virus isolationrt-pcrserbiaVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 4, Pp 509-519 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine parainfluenza virus type 3 virus isolation rt-pcr serbia Veterinary medicine SF600-1100
spellingShingle	bovine parainfluenza virus type 3 virus isolation rt-pcr serbia Veterinary medicine SF600-1100 Veljović Ljubiša Knežević Aleksandra Milić Nenad Krnjaić Dejan Miković Radoš Zorić Andrea Marković Maja Milićević Vesna Stamenković Miodrag Stanojević Maja Maksimović-Zorić Jelena Petrović Tamaš Nišavić Jakov Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia
description	The presence of bovine parainfluenza virus type 3 (BPIV3) was examined in 119 nasal swabs collected from cattle with severe respiratory infection. All samples were conducted for virus isolation on the MDBK cell line. The cytopathic effect was observed after 48h to 72h in cells inoculated with eight samples (8/119; 6.7%). The confirmation of isolated strains of BPIV3 was done by the virus-neutralization test. In addition, all samples of bovine nasal swabs were also examined for the presence of BPIV3 virus using RT-PCR with primers specific for the part of HN gene. The presence of BPIV3 was detected in eight samples (8/119; 6.7%) that were also positive upon virus isolation. The molecular characterization based on nucleotide sequencing of the part of the HN gene showed that all BPIV3 isolates belonged to genotype C of BPIV3. They branched in one distinct cluster with three different branches, but these branches were very similar to each other (98.1% to 99.8%). Serbian BPIV3c isolates were most similar to the Chinese BPIV3c isolates SD0805, SD0809 and SD0835 (from 97.92% to 99.7%), and to South Korean (12Q061), Japanese (HS9) and American (TVMDL16 and TVMDL20) BPIV3c strains (from 97.1% to 98.8%), and distinct from American (TVMDL15and TVMDL17) and Australian (Q5592) BPI3V genotype B strains (only 79.9% to 82.3% similarity), as well as from the genotype A BPIV3 strains from different countries published in GenBank.
format	article
author	Veljović Ljubiša Knežević Aleksandra Milić Nenad Krnjaić Dejan Miković Radoš Zorić Andrea Marković Maja Milićević Vesna Stamenković Miodrag Stanojević Maja Maksimović-Zorić Jelena Petrović Tamaš Nišavić Jakov
author_facet	Veljović Ljubiša Knežević Aleksandra Milić Nenad Krnjaić Dejan Miković Radoš Zorić Andrea Marković Maja Milićević Vesna Stamenković Miodrag Stanojević Maja Maksimović-Zorić Jelena Petrović Tamaš Nišavić Jakov
author_sort	Veljović Ljubiša
title	Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia
title_short	Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia
title_full	Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia
title_fullStr	Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia
title_full_unstemmed	Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia
title_sort	isolation and molecular detection of bovine parainfluenza virus type 3 in cattle in serbia
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/5cc1fc8d8f8c4b3495cc9b1f1a9d2acd
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Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

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