Presence of diabetes autoantigens in extracellular vesicles derived from human islets

Abstract Beta-cell (β-cell) injury is the hallmark of autoimmune diabetes. However, the mechanisms by which autoreactive responses are generated in susceptible individuals are not well understood. Extracellular vesicles (EV) are produced by mammalian cells under normal and stressed physiological sta...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Craig P. Hasilo, Sarita Negi, Isabelle Allaeys, Nathalie Cloutier, Alissa K. Rutman, Marco Gasparrini, Éric Bonneil, Pierre Thibault, Éric Boilard, Steven Paraskevas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cc65e26c9a04fa5a780db8b66742e77
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5cc65e26c9a04fa5a780db8b66742e77
record_format	dspace
spelling	oai:doaj.org-article:5cc65e26c9a04fa5a780db8b66742e772021-12-02T12:32:41ZPresence of diabetes autoantigens in extracellular vesicles derived from human islets10.1038/s41598-017-04977-y2045-2322https://doaj.org/article/5cc65e26c9a04fa5a780db8b66742e772017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04977-yhttps://doaj.org/toc/2045-2322Abstract Beta-cell (β-cell) injury is the hallmark of autoimmune diabetes. However, the mechanisms by which autoreactive responses are generated in susceptible individuals are not well understood. Extracellular vesicles (EV) are produced by mammalian cells under normal and stressed physiological states. They are an important part of cellular communication, and may serve a role in antigen processing and presentation. We hypothesized that isolated human islets in culture produce EV that contain diabetes autoantigens (DAA) from these otherwise normal, non-diabetic donors. Here we report the caspase-independent production of EV by human islets in culture, and the characterization of DAA glutamic acid decarboxylase 65 (GAD65) and zinc transporter 8 (ZnT8), as well as the β-cell resident glucose transporter 2 (Glut2), present within the EV.Craig P. HasiloSarita NegiIsabelle AllaeysNathalie CloutierAlissa K. RutmanMarco GasparriniÉric BonneilPierre ThibaultÉric BoilardSteven ParaskevasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Craig P. Hasilo Sarita Negi Isabelle Allaeys Nathalie Cloutier Alissa K. Rutman Marco Gasparrini Éric Bonneil Pierre Thibault Éric Boilard Steven Paraskevas Presence of diabetes autoantigens in extracellular vesicles derived from human islets
description	Abstract Beta-cell (β-cell) injury is the hallmark of autoimmune diabetes. However, the mechanisms by which autoreactive responses are generated in susceptible individuals are not well understood. Extracellular vesicles (EV) are produced by mammalian cells under normal and stressed physiological states. They are an important part of cellular communication, and may serve a role in antigen processing and presentation. We hypothesized that isolated human islets in culture produce EV that contain diabetes autoantigens (DAA) from these otherwise normal, non-diabetic donors. Here we report the caspase-independent production of EV by human islets in culture, and the characterization of DAA glutamic acid decarboxylase 65 (GAD65) and zinc transporter 8 (ZnT8), as well as the β-cell resident glucose transporter 2 (Glut2), present within the EV.
format	article
author	Craig P. Hasilo Sarita Negi Isabelle Allaeys Nathalie Cloutier Alissa K. Rutman Marco Gasparrini Éric Bonneil Pierre Thibault Éric Boilard Steven Paraskevas
author_facet	Craig P. Hasilo Sarita Negi Isabelle Allaeys Nathalie Cloutier Alissa K. Rutman Marco Gasparrini Éric Bonneil Pierre Thibault Éric Boilard Steven Paraskevas
author_sort	Craig P. Hasilo
title	Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_short	Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_full	Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_fullStr	Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_full_unstemmed	Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_sort	presence of diabetes autoantigens in extracellular vesicles derived from human islets
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5cc65e26c9a04fa5a780db8b66742e77
work_keys_str_mv	AT craigphasilo presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT saritanegi presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT isabelleallaeys presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT nathaliecloutier presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT alissakrutman presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT marcogasparrini presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT ericbonneil presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT pierrethibault presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT ericboilard presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets AT stevenparaskevas presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
_version_	1718393980212740096

Presence of diabetes autoantigens in extracellular vesicles derived from human islets

Ejemplares similares