Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway

Context: Morphine is an alkaloid isolated from the poppy plants. The addiction of morphine is a very serious social issue. Some long non-coding RNAs (lncRNAs) have been proposed to engage in drug addiction. Objective: Whether lncRNA maternally expressed gene 3 (MEG3) attended to morphine-mediated au...

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Autores principales:	Shuibo Gao, Enyao Li, Haixia Gao
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	drug addiction morphine cell autophagy lncrna maternally expressed gene 3 orexin1 receptor Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5cc83d33631546bbb1696f147f090cc0
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spelling	oai:doaj.org-article:5cc83d33631546bbb1696f147f090cc02021-11-17T14:21:56ZLong non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway1388-02091744-511610.1080/13880209.2019.1651343https://doaj.org/article/5cc83d33631546bbb1696f147f090cc02019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1651343https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Morphine is an alkaloid isolated from the poppy plants. The addiction of morphine is a very serious social issue. Some long non-coding RNAs (lncRNAs) have been proposed to engage in drug addiction. Objective: Whether lncRNA maternally expressed gene 3 (MEG3) attended to morphine-mediated autophagy of mouse hippocampal neuronal HT22 cells was probed. Materials and methods: HT22 cells were subjected to 10 µM morphine for 24 h. Cell autophagy was assessed by measuring LC3-II/LC3-I and Beclin-1 expression. qRT-PCR was carried out to measure MEG3 expression. SiRNA oligoribonucleotides targeting MEG3 (si-MEG3) was transfected to silence MEG3. The orexin1 receptor (OX1R), c-fos, p/t-ERK and p/t-PKC expressions were tested by western blotting. SCH772984 was used as an inhibitor of ERK pathway. Results: Morphine elevated OX1R (2.92 times), c-fos (2.06 times), p/t-ERK (2.04 times) and p/t-PKC (2.4 times), Beclin-1 (3.2 times) and LC3-II/LC3-I (3.96 times) expression in HT22 cells. Moreover, followed by morphine exposure, the MEG3 expression was also elevated in HT22 cells (3.03 times). The silence of MEG3 lowered the Beclin-1 (1.85 times), LC3-II/LC3-I (2.12 times), c-fos (1.39 times) and p/t-ERK (1.44 times) expressions in morphine-treated HT22 cells. Inhibitor of ERK pathway SCH772984 further promoted the influence of MEG3 silence on morphine-caused Beclin-1 (1.97 times) and LC3-II/LC3-I (1.92 times) expressions decreases. Conclusions: Up-regulation of MEG3 attended to the morphine-caused autophagy of HT22 cells might be through elevating c-fos expression and promoting ERK pathway activation. More experiments are also needed in the future to analyse the influence of other lncRNAs in drug addiction.Shuibo GaoEnyao LiHaixia GaoTaylor & Francis Grouparticledrug addictionmorphinecell autophagylncrna maternally expressed gene 3orexin1 receptorTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 536-542 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	drug addiction morphine cell autophagy lncrna maternally expressed gene 3 orexin1 receptor Therapeutics. Pharmacology RM1-950
spellingShingle	drug addiction morphine cell autophagy lncrna maternally expressed gene 3 orexin1 receptor Therapeutics. Pharmacology RM1-950 Shuibo Gao Enyao Li Haixia Gao Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
description	Context: Morphine is an alkaloid isolated from the poppy plants. The addiction of morphine is a very serious social issue. Some long non-coding RNAs (lncRNAs) have been proposed to engage in drug addiction. Objective: Whether lncRNA maternally expressed gene 3 (MEG3) attended to morphine-mediated autophagy of mouse hippocampal neuronal HT22 cells was probed. Materials and methods: HT22 cells were subjected to 10 µM morphine for 24 h. Cell autophagy was assessed by measuring LC3-II/LC3-I and Beclin-1 expression. qRT-PCR was carried out to measure MEG3 expression. SiRNA oligoribonucleotides targeting MEG3 (si-MEG3) was transfected to silence MEG3. The orexin1 receptor (OX1R), c-fos, p/t-ERK and p/t-PKC expressions were tested by western blotting. SCH772984 was used as an inhibitor of ERK pathway. Results: Morphine elevated OX1R (2.92 times), c-fos (2.06 times), p/t-ERK (2.04 times) and p/t-PKC (2.4 times), Beclin-1 (3.2 times) and LC3-II/LC3-I (3.96 times) expression in HT22 cells. Moreover, followed by morphine exposure, the MEG3 expression was also elevated in HT22 cells (3.03 times). The silence of MEG3 lowered the Beclin-1 (1.85 times), LC3-II/LC3-I (2.12 times), c-fos (1.39 times) and p/t-ERK (1.44 times) expressions in morphine-treated HT22 cells. Inhibitor of ERK pathway SCH772984 further promoted the influence of MEG3 silence on morphine-caused Beclin-1 (1.97 times) and LC3-II/LC3-I (1.92 times) expressions decreases. Conclusions: Up-regulation of MEG3 attended to the morphine-caused autophagy of HT22 cells might be through elevating c-fos expression and promoting ERK pathway activation. More experiments are also needed in the future to analyse the influence of other lncRNAs in drug addiction.
format	article
author	Shuibo Gao Enyao Li Haixia Gao
author_facet	Shuibo Gao Enyao Li Haixia Gao
author_sort	Shuibo Gao
title	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
title_short	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
title_full	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
title_fullStr	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
title_full_unstemmed	Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway
title_sort	long non-coding rna meg3 attends to morphine-mediated autophagy of ht22 cells through modulating erk pathway
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/5cc83d33631546bbb1696f147f090cc0
work_keys_str_mv	AT shuibogao longnoncodingrnameg3attendstomorphinemediatedautophagyofht22cellsthroughmodulatingerkpathway AT enyaoli longnoncodingrnameg3attendstomorphinemediatedautophagyofht22cellsthroughmodulatingerkpathway AT haixiagao longnoncodingrnameg3attendstomorphinemediatedautophagyofht22cellsthroughmodulatingerkpathway
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Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway

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