Regulation of glycolysis in brown adipocytes by HIF-1α

Abstract Brown adipose tissue takes up large amounts of glucose during cold exposure in mice and humans. Here we report an induction of glucose transporter 1 expression and increased expression of several glycolytic enzymes in brown adipose tissue from cold-exposed mice. Accordingly, these genes wer...

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Autores principales:	Astrid L. Basse, Marie S. Isidor, Sally Winther, Nina B. Skjoldborg, Maria Murholm, Elise S. Andersen, Steen B. Pedersen, Christian Wolfrum, Bjørn Quistorff, Jacob B. Hansen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cc8df8a3be24f4c90f043fd2f9aa223
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spelling	oai:doaj.org-article:5cc8df8a3be24f4c90f043fd2f9aa2232021-12-02T11:52:37ZRegulation of glycolysis in brown adipocytes by HIF-1α10.1038/s41598-017-04246-y2045-2322https://doaj.org/article/5cc8df8a3be24f4c90f043fd2f9aa2232017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04246-yhttps://doaj.org/toc/2045-2322Abstract Brown adipose tissue takes up large amounts of glucose during cold exposure in mice and humans. Here we report an induction of glucose transporter 1 expression and increased expression of several glycolytic enzymes in brown adipose tissue from cold-exposed mice. Accordingly, these genes were also induced after β-adrenergic activation of cultured brown adipocytes, concomitant with accumulation of hypoxia inducible factor-1α (HIF-1α) protein levels. HIF-1α accumulation was dependent on uncoupling protein 1 and generation of mitochondrial reactive oxygen species. Expression of key glycolytic enzymes was reduced after knockdown of HIF-1α in mature brown adipocytes. Glucose consumption, lactate export and glycolytic capacity were reduced in brown adipocytes depleted of Hif-1α. Finally, we observed a decreased β-adrenergically induced oxygen consumption in Hif-1α knockdown adipocytes cultured in medium with glucose as the only exogenously added fuel. These data suggest that HIF-1α-dependent regulation of glycolysis is necessary for maximum glucose metabolism in brown adipocytes.Astrid L. BasseMarie S. IsidorSally WintherNina B. SkjoldborgMaria MurholmElise S. AndersenSteen B. PedersenChristian WolfrumBjørn QuistorffJacob B. HansenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Astrid L. Basse Marie S. Isidor Sally Winther Nina B. Skjoldborg Maria Murholm Elise S. Andersen Steen B. Pedersen Christian Wolfrum Bjørn Quistorff Jacob B. Hansen Regulation of glycolysis in brown adipocytes by HIF-1α
description	Abstract Brown adipose tissue takes up large amounts of glucose during cold exposure in mice and humans. Here we report an induction of glucose transporter 1 expression and increased expression of several glycolytic enzymes in brown adipose tissue from cold-exposed mice. Accordingly, these genes were also induced after β-adrenergic activation of cultured brown adipocytes, concomitant with accumulation of hypoxia inducible factor-1α (HIF-1α) protein levels. HIF-1α accumulation was dependent on uncoupling protein 1 and generation of mitochondrial reactive oxygen species. Expression of key glycolytic enzymes was reduced after knockdown of HIF-1α in mature brown adipocytes. Glucose consumption, lactate export and glycolytic capacity were reduced in brown adipocytes depleted of Hif-1α. Finally, we observed a decreased β-adrenergically induced oxygen consumption in Hif-1α knockdown adipocytes cultured in medium with glucose as the only exogenously added fuel. These data suggest that HIF-1α-dependent regulation of glycolysis is necessary for maximum glucose metabolism in brown adipocytes.
format	article
author	Astrid L. Basse Marie S. Isidor Sally Winther Nina B. Skjoldborg Maria Murholm Elise S. Andersen Steen B. Pedersen Christian Wolfrum Bjørn Quistorff Jacob B. Hansen
author_facet	Astrid L. Basse Marie S. Isidor Sally Winther Nina B. Skjoldborg Maria Murholm Elise S. Andersen Steen B. Pedersen Christian Wolfrum Bjørn Quistorff Jacob B. Hansen
author_sort	Astrid L. Basse
title	Regulation of glycolysis in brown adipocytes by HIF-1α
title_short	Regulation of glycolysis in brown adipocytes by HIF-1α
title_full	Regulation of glycolysis in brown adipocytes by HIF-1α
title_fullStr	Regulation of glycolysis in brown adipocytes by HIF-1α
title_full_unstemmed	Regulation of glycolysis in brown adipocytes by HIF-1α
title_sort	regulation of glycolysis in brown adipocytes by hif-1α
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5cc8df8a3be24f4c90f043fd2f9aa223
work_keys_str_mv	AT astridlbasse regulationofglycolysisinbrownadipocytesbyhif1a AT mariesisidor regulationofglycolysisinbrownadipocytesbyhif1a AT sallywinther regulationofglycolysisinbrownadipocytesbyhif1a AT ninabskjoldborg regulationofglycolysisinbrownadipocytesbyhif1a AT mariamurholm regulationofglycolysisinbrownadipocytesbyhif1a AT elisesandersen regulationofglycolysisinbrownadipocytesbyhif1a AT steenbpedersen regulationofglycolysisinbrownadipocytesbyhif1a AT christianwolfrum regulationofglycolysisinbrownadipocytesbyhif1a AT bjørnquistorff regulationofglycolysisinbrownadipocytesbyhif1a AT jacobbhansen regulationofglycolysisinbrownadipocytesbyhif1a
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Regulation of glycolysis in brown adipocytes by HIF-1α

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