Examining cellular responses to reconstituted antibody protein liquids

Abstract Protein ionic liquids (PIL) are a new class of biologic stabilizers designed to protect the functionality and extend the shelf-life of biotechnological and therapeutic agents making them more readily available, and resistant to austere environments. Protein biorecognition elements such as m...

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Autores principales:	M. Tyler Nelson, Joseph M. Slocik, Eric J. Romer, Cassandra I. Mankus, Richard T. Agans, Rajesh R. Naik, Saber M. Hussain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ccef12d0de0453891bedc6f18d5e2aa
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spelling	oai:doaj.org-article:5ccef12d0de0453891bedc6f18d5e2aa2021-12-02T15:09:23ZExamining cellular responses to reconstituted antibody protein liquids10.1038/s41598-021-96375-82045-2322https://doaj.org/article/5ccef12d0de0453891bedc6f18d5e2aa2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96375-8https://doaj.org/toc/2045-2322Abstract Protein ionic liquids (PIL) are a new class of biologic stabilizers designed to protect the functionality and extend the shelf-life of biotechnological and therapeutic agents making them more readily available, and resistant to austere environments. Protein biorecognition elements such as monoclonal antibodies are commonly utilized therapeutics that require the robust stabilization offered by PILs, but biocompatibility remains an important issue. This study has focused on characterizing the biocompatibility of an antibody based PIL by exposing multiple cells types to a cationized immunoglobulin suspended in an anionic liquid (IgG-IL). The IgG-IL caused no significant alterations in cellular health for all three cell types with treatments < 12.5 µg/mL. Concentrations ≥ 12.5 µg/mL resulted in significant necrotic cell death in A549 and HaCaT cells, and caspase associated cell death in HepG2 cells. In addition, all cells displayed evidence of oxidative stress and IL-8 induction in response to IgG-IL exposures. Therapeutic Ig can be utilized with a wide dose range that extends into concentrations we have found to exhibit cytotoxicity raising a toxicity concern and a need for more extensive understanding of the biocompatibility of IgG-ILs.M. Tyler NelsonJoseph M. SlocikEric J. RomerCassandra I. MankusRichard T. AgansRajesh R. NaikSaber M. HussainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Tyler Nelson Joseph M. Slocik Eric J. Romer Cassandra I. Mankus Richard T. Agans Rajesh R. Naik Saber M. Hussain Examining cellular responses to reconstituted antibody protein liquids
description	Abstract Protein ionic liquids (PIL) are a new class of biologic stabilizers designed to protect the functionality and extend the shelf-life of biotechnological and therapeutic agents making them more readily available, and resistant to austere environments. Protein biorecognition elements such as monoclonal antibodies are commonly utilized therapeutics that require the robust stabilization offered by PILs, but biocompatibility remains an important issue. This study has focused on characterizing the biocompatibility of an antibody based PIL by exposing multiple cells types to a cationized immunoglobulin suspended in an anionic liquid (IgG-IL). The IgG-IL caused no significant alterations in cellular health for all three cell types with treatments < 12.5 µg/mL. Concentrations ≥ 12.5 µg/mL resulted in significant necrotic cell death in A549 and HaCaT cells, and caspase associated cell death in HepG2 cells. In addition, all cells displayed evidence of oxidative stress and IL-8 induction in response to IgG-IL exposures. Therapeutic Ig can be utilized with a wide dose range that extends into concentrations we have found to exhibit cytotoxicity raising a toxicity concern and a need for more extensive understanding of the biocompatibility of IgG-ILs.
format	article
author	M. Tyler Nelson Joseph M. Slocik Eric J. Romer Cassandra I. Mankus Richard T. Agans Rajesh R. Naik Saber M. Hussain
author_facet	M. Tyler Nelson Joseph M. Slocik Eric J. Romer Cassandra I. Mankus Richard T. Agans Rajesh R. Naik Saber M. Hussain
author_sort	M. Tyler Nelson
title	Examining cellular responses to reconstituted antibody protein liquids
title_short	Examining cellular responses to reconstituted antibody protein liquids
title_full	Examining cellular responses to reconstituted antibody protein liquids
title_fullStr	Examining cellular responses to reconstituted antibody protein liquids
title_full_unstemmed	Examining cellular responses to reconstituted antibody protein liquids
title_sort	examining cellular responses to reconstituted antibody protein liquids
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5ccef12d0de0453891bedc6f18d5e2aa
work_keys_str_mv	AT mtylernelson examiningcellularresponsestoreconstitutedantibodyproteinliquids AT josephmslocik examiningcellularresponsestoreconstitutedantibodyproteinliquids AT ericjromer examiningcellularresponsestoreconstitutedantibodyproteinliquids AT cassandraimankus examiningcellularresponsestoreconstitutedantibodyproteinliquids AT richardtagans examiningcellularresponsestoreconstitutedantibodyproteinliquids AT rajeshrnaik examiningcellularresponsestoreconstitutedantibodyproteinliquids AT sabermhussain examiningcellularresponsestoreconstitutedantibodyproteinliquids
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Examining cellular responses to reconstituted antibody protein liquids

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