Examining cellular responses to reconstituted antibody protein liquids

Abstract Protein ionic liquids (PIL) are a new class of biologic stabilizers designed to protect the functionality and extend the shelf-life of biotechnological and therapeutic agents making them more readily available, and resistant to austere environments. Protein biorecognition elements such as m...

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Autores principales: M. Tyler Nelson, Joseph M. Slocik, Eric J. Romer, Cassandra I. Mankus, Richard T. Agans, Rajesh R. Naik, Saber M. Hussain
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/5ccef12d0de0453891bedc6f18d5e2aa
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spelling oai:doaj.org-article:5ccef12d0de0453891bedc6f18d5e2aa2021-12-02T15:09:23ZExamining cellular responses to reconstituted antibody protein liquids10.1038/s41598-021-96375-82045-2322https://doaj.org/article/5ccef12d0de0453891bedc6f18d5e2aa2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96375-8https://doaj.org/toc/2045-2322Abstract Protein ionic liquids (PIL) are a new class of biologic stabilizers designed to protect the functionality and extend the shelf-life of biotechnological and therapeutic agents making them more readily available, and resistant to austere environments. Protein biorecognition elements such as monoclonal antibodies are commonly utilized therapeutics that require the robust stabilization offered by PILs, but biocompatibility remains an important issue. This study has focused on characterizing the biocompatibility of an antibody based PIL by exposing multiple cells types to a cationized immunoglobulin suspended in an anionic liquid (IgG-IL). The IgG-IL caused no significant alterations in cellular health for all three cell types with treatments < 12.5 µg/mL. Concentrations ≥ 12.5 µg/mL resulted in significant necrotic cell death in A549 and HaCaT cells, and caspase associated cell death in HepG2 cells. In addition, all cells displayed evidence of oxidative stress and IL-8 induction in response to IgG-IL exposures. Therapeutic Ig can be utilized with a wide dose range that extends into concentrations we have found to exhibit cytotoxicity raising a toxicity concern and a need for more extensive understanding of the biocompatibility of IgG-ILs.M. Tyler NelsonJoseph M. SlocikEric J. RomerCassandra I. MankusRichard T. AgansRajesh R. NaikSaber M. HussainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
M. Tyler Nelson
Joseph M. Slocik
Eric J. Romer
Cassandra I. Mankus
Richard T. Agans
Rajesh R. Naik
Saber M. Hussain
Examining cellular responses to reconstituted antibody protein liquids
description Abstract Protein ionic liquids (PIL) are a new class of biologic stabilizers designed to protect the functionality and extend the shelf-life of biotechnological and therapeutic agents making them more readily available, and resistant to austere environments. Protein biorecognition elements such as monoclonal antibodies are commonly utilized therapeutics that require the robust stabilization offered by PILs, but biocompatibility remains an important issue. This study has focused on characterizing the biocompatibility of an antibody based PIL by exposing multiple cells types to a cationized immunoglobulin suspended in an anionic liquid (IgG-IL). The IgG-IL caused no significant alterations in cellular health for all three cell types with treatments < 12.5 µg/mL. Concentrations ≥ 12.5 µg/mL resulted in significant necrotic cell death in A549 and HaCaT cells, and caspase associated cell death in HepG2 cells. In addition, all cells displayed evidence of oxidative stress and IL-8 induction in response to IgG-IL exposures. Therapeutic Ig can be utilized with a wide dose range that extends into concentrations we have found to exhibit cytotoxicity raising a toxicity concern and a need for more extensive understanding of the biocompatibility of IgG-ILs.
format article
author M. Tyler Nelson
Joseph M. Slocik
Eric J. Romer
Cassandra I. Mankus
Richard T. Agans
Rajesh R. Naik
Saber M. Hussain
author_facet M. Tyler Nelson
Joseph M. Slocik
Eric J. Romer
Cassandra I. Mankus
Richard T. Agans
Rajesh R. Naik
Saber M. Hussain
author_sort M. Tyler Nelson
title Examining cellular responses to reconstituted antibody protein liquids
title_short Examining cellular responses to reconstituted antibody protein liquids
title_full Examining cellular responses to reconstituted antibody protein liquids
title_fullStr Examining cellular responses to reconstituted antibody protein liquids
title_full_unstemmed Examining cellular responses to reconstituted antibody protein liquids
title_sort examining cellular responses to reconstituted antibody protein liquids
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/5ccef12d0de0453891bedc6f18d5e2aa
work_keys_str_mv AT mtylernelson examiningcellularresponsestoreconstitutedantibodyproteinliquids
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AT ericjromer examiningcellularresponsestoreconstitutedantibodyproteinliquids
AT cassandraimankus examiningcellularresponsestoreconstitutedantibodyproteinliquids
AT richardtagans examiningcellularresponsestoreconstitutedantibodyproteinliquids
AT rajeshrnaik examiningcellularresponsestoreconstitutedantibodyproteinliquids
AT sabermhussain examiningcellularresponsestoreconstitutedantibodyproteinliquids
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