Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor

BACKGROUND: Cryptosporidium spp. is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp. can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patien...

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Autores principales:	Mehmet Karabey, Hüseyin Can, Tülay Öncü Öner, Mert Döşkaya, Sedef Erkunt Alak, Aysu Değirmenci Döşkaya, Muhammet Karakavuk, Ahmet Efe Köseoğlu, Cemal Ün, Adnan Yüksel Gürüz, Ahmet Alacacıoğlu, Bayram Pektaş, Aytül Gül, Selçuk Kaya, Ayşegül Aksoy Gökmen
Formato:	article
Lenguaje:	EN
Publicado:	King Faisal Specialist Hospital and Research Centre 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/5ce0dc472305430a993b3e4e3bd7da7c
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spelling	oai:doaj.org-article:5ce0dc472305430a993b3e4e3bd7da7c2021-12-01T12:57:42ZCryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor10.5144/0256-4947.2021.2930975-44660256-4947https://doaj.org/article/5ce0dc472305430a993b3e4e3bd7da7c2021-09-01T00:00:00Zhttp://www.annsaudimed.net/doi/10.5144/0256-4947.2021.293https://doaj.org/toc/0256-4947https://doaj.org/toc/0975-4466BACKGROUND: Cryptosporidium spp. is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp. can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patients undergoing chemotherapy for malignant solid tumors. OBJECTIVE: Investigate the prevalence of Cryptosporidium spp. in stool samples. DESIGN: Cross-sectional. SETTING: Tertiary care. PATIENTS AND METHODS: Stool samples were collected from adult patients with malignant solid tumors receiving chemotherapy and diarrhea. Cryptosporidium spp. prevalence was determined using Ziehl–Neelsen staining, ELISA, and real-time PCR targeting of the COWP gene. MAIN OUTCOME MEASURE: The prevalence of Cryptosporidium spp. in patients undergoing chemotherapy for malignant solid tumors. SAMPLE SIZE: 94 RESULTS: The prevalence was 2.1% (2/94), 5.3% (5/94), and 5.3% (5/94) as detected by Ziehl–Neelsen staining, real-time PCR and ELISA, respectively. The prevalence reached 8.5% (8/94) using all results obtained from the three methods. Among eight positive stool samples, four were positive by at least two different methods (Ziehl–Neelsen staining-ELISA or ELISA-real-time PCR) whereas the remaining four were positive by either ELISA or real-time PCR. CONCLUSION: These findings show the risk of cryptosporidiosis in cancer patients and the necessity to use at least two diagnostic methods during the diagnosis of cryptosporidiosis to reach more accurate and trustworthy results. LIMITATIONS: Further studies with a larger sample size are recommended. CONFLICT OF INTEREST: None.Mehmet KarabeyHüseyin CanTülay Öncü ÖnerMert DöşkayaSedef Erkunt AlakAysu Değirmenci DöşkayaMuhammet KarakavukAhmet Efe KöseoğluCemal ÜnAdnan Yüksel GürüzAhmet AlacacıoğluBayram PektaşAytül GülSelçuk KayaAyşegül Aksoy GökmenKing Faisal Specialist Hospital and Research CentrearticleMedicineRENAnnals of Saudi Medicine, Vol 41, Iss 5, Pp 293-298 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Mehmet Karabey Hüseyin Can Tülay Öncü Öner Mert Döşkaya Sedef Erkunt Alak Aysu Değirmenci Döşkaya Muhammet Karakavuk Ahmet Efe Köseoğlu Cemal Ün Adnan Yüksel Gürüz Ahmet Alacacıoğlu Bayram Pektaş Aytül Gül Selçuk Kaya Ayşegül Aksoy Gökmen Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
description	BACKGROUND: Cryptosporidium spp. is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp. can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patients undergoing chemotherapy for malignant solid tumors. OBJECTIVE: Investigate the prevalence of Cryptosporidium spp. in stool samples. DESIGN: Cross-sectional. SETTING: Tertiary care. PATIENTS AND METHODS: Stool samples were collected from adult patients with malignant solid tumors receiving chemotherapy and diarrhea. Cryptosporidium spp. prevalence was determined using Ziehl–Neelsen staining, ELISA, and real-time PCR targeting of the COWP gene. MAIN OUTCOME MEASURE: The prevalence of Cryptosporidium spp. in patients undergoing chemotherapy for malignant solid tumors. SAMPLE SIZE: 94 RESULTS: The prevalence was 2.1% (2/94), 5.3% (5/94), and 5.3% (5/94) as detected by Ziehl–Neelsen staining, real-time PCR and ELISA, respectively. The prevalence reached 8.5% (8/94) using all results obtained from the three methods. Among eight positive stool samples, four were positive by at least two different methods (Ziehl–Neelsen staining-ELISA or ELISA-real-time PCR) whereas the remaining four were positive by either ELISA or real-time PCR. CONCLUSION: These findings show the risk of cryptosporidiosis in cancer patients and the necessity to use at least two diagnostic methods during the diagnosis of cryptosporidiosis to reach more accurate and trustworthy results. LIMITATIONS: Further studies with a larger sample size are recommended. CONFLICT OF INTEREST: None.
format	article
author	Mehmet Karabey Hüseyin Can Tülay Öncü Öner Mert Döşkaya Sedef Erkunt Alak Aysu Değirmenci Döşkaya Muhammet Karakavuk Ahmet Efe Köseoğlu Cemal Ün Adnan Yüksel Gürüz Ahmet Alacacıoğlu Bayram Pektaş Aytül Gül Selçuk Kaya Ayşegül Aksoy Gökmen
author_facet	Mehmet Karabey Hüseyin Can Tülay Öncü Öner Mert Döşkaya Sedef Erkunt Alak Aysu Değirmenci Döşkaya Muhammet Karakavuk Ahmet Efe Köseoğlu Cemal Ün Adnan Yüksel Gürüz Ahmet Alacacıoğlu Bayram Pektaş Aytül Gül Selçuk Kaya Ayşegül Aksoy Gökmen
author_sort	Mehmet Karabey
title	Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
title_short	Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
title_full	Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
title_fullStr	Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
title_full_unstemmed	Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
title_sort	cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
publisher	King Faisal Specialist Hospital and Research Centre
publishDate	2021
url	https://doaj.org/article/5ce0dc472305430a993b3e4e3bd7da7c
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Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor

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