Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts

In this study, we propose a chronobiological method for examining inter-microbial interactions in bacterial and fungal associations in female reproductive tract. Fungal and bacterial species were isolated in 45 women of reproductive aged 19–35, with regular menstrual cycle, applying no hormonal cont...

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Autores principales:	M. V. Nikolenko, N. V. Baryshnikova, E. A. Bakhlykova, A. V. Zykova, K. V. Romanchenko
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	candida albicans microsymbionts dysbiosis mesor acrophase amplitude-phase parameters Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/5cf1c99c32b740f9b90876940b514a76
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spelling	oai:doaj.org-article:5cf1c99c32b740f9b90876940b514a762021-11-22T07:09:53ZBioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts2220-76192313-739810.15789/2220-7619-2019-2-363-368https://doaj.org/article/5cf1c99c32b740f9b90876940b514a762019-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/705https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398In this study, we propose a chronobiological method for examining inter-microbial interactions in bacterial and fungal associations in female reproductive tract. Fungal and bacterial species were isolated in 45 women of reproductive aged 19–35, with regular menstrual cycle, applying no hormonal contraceptives, without previous gynecological surgery, abortions, miscarriages with vaginal eubiosis and dysbiosis in history. Sexually transmitted diseases (HIV infection, syphilis, gonorrhea, trichomoniasis, chlamydiosis) were excluded in all subjects. Proliferation rate, morphogenesis and phospholipase activity were examined within the 48-hour period every 4 hours, in winter time, Moon phase IV. The data obtained were assessed by using Student’s t-test, Wilcoxon test, and least squares method. All subjects were divided into the groups: group 1 — women with vaginal eubiosis, group 2 — women with vaginal dysbiosis. It was shown that in all subjects experimental parameters of C. albicans cultures showed a diurnal dynamics characterized in healthy women by circadian rhythms with a single peak of activity. However, in women with vaginal dysbiosis C. albicans was characterized by significant ultradian (around 12 hours long) rhythms with two peaks of biological activity. Concurrence and consistency in manifested physiological functions related to clinical isolates was coupled to temporal pattern of distributed biological resources in fungi depending on course of infectious process. It was found that in vaginal eubiosis exometabolites released by dominant associated microbiota did not significantly change microbiota-related amplitude-phase parameters. The data obtained evidenced that temporal pattern of parameters related to C. albicans from healthy individuals was stable and independent on bacterial metabolites. In contrast, dominant microsymbiont in vaginal dysbiosis inhibited fungi-related rhythms, which might be important in establishing lactobacillus-associated biotope colonization resistance. Effects of metabolites released by the associated microbiota typical to dysbiosis was revealed by increased mesor, amplitude, preserved biorhythm spectral pattern in examined properties as well as amplitude-phase characteristics indicating at enhanced or sustained C. albicans adaptive potential. Therefore, the amplitude-phase parameter of C. albicans physiological activity served as a marker of opposite (enhanced/weakened) effect of microsymbiont survival described in “microbial dominant-associate” pairs.M. V. NikolenkoN. V. BaryshnikovaE. A. BakhlykovaA. V. ZykovaK. V. RomanchenkoSankt-Peterburg : NIIÈM imeni Pasteraarticlecandida albicansmicrosymbiontsdysbiosismesoracrophaseamplitude-phase parametersInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 2, Pp 363-368 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	candida albicans microsymbionts dysbiosis mesor acrophase amplitude-phase parameters Infectious and parasitic diseases RC109-216
spellingShingle	candida albicans microsymbionts dysbiosis mesor acrophase amplitude-phase parameters Infectious and parasitic diseases RC109-216 M. V. Nikolenko N. V. Baryshnikova E. A. Bakhlykova A. V. Zykova K. V. Romanchenko Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
description	In this study, we propose a chronobiological method for examining inter-microbial interactions in bacterial and fungal associations in female reproductive tract. Fungal and bacterial species were isolated in 45 women of reproductive aged 19–35, with regular menstrual cycle, applying no hormonal contraceptives, without previous gynecological surgery, abortions, miscarriages with vaginal eubiosis and dysbiosis in history. Sexually transmitted diseases (HIV infection, syphilis, gonorrhea, trichomoniasis, chlamydiosis) were excluded in all subjects. Proliferation rate, morphogenesis and phospholipase activity were examined within the 48-hour period every 4 hours, in winter time, Moon phase IV. The data obtained were assessed by using Student’s t-test, Wilcoxon test, and least squares method. All subjects were divided into the groups: group 1 — women with vaginal eubiosis, group 2 — women with vaginal dysbiosis. It was shown that in all subjects experimental parameters of C. albicans cultures showed a diurnal dynamics characterized in healthy women by circadian rhythms with a single peak of activity. However, in women with vaginal dysbiosis C. albicans was characterized by significant ultradian (around 12 hours long) rhythms with two peaks of biological activity. Concurrence and consistency in manifested physiological functions related to clinical isolates was coupled to temporal pattern of distributed biological resources in fungi depending on course of infectious process. It was found that in vaginal eubiosis exometabolites released by dominant associated microbiota did not significantly change microbiota-related amplitude-phase parameters. The data obtained evidenced that temporal pattern of parameters related to C. albicans from healthy individuals was stable and independent on bacterial metabolites. In contrast, dominant microsymbiont in vaginal dysbiosis inhibited fungi-related rhythms, which might be important in establishing lactobacillus-associated biotope colonization resistance. Effects of metabolites released by the associated microbiota typical to dysbiosis was revealed by increased mesor, amplitude, preserved biorhythm spectral pattern in examined properties as well as amplitude-phase characteristics indicating at enhanced or sustained C. albicans adaptive potential. Therefore, the amplitude-phase parameter of C. albicans physiological activity served as a marker of opposite (enhanced/weakened) effect of microsymbiont survival described in “microbial dominant-associate” pairs.
format	article
author	M. V. Nikolenko N. V. Baryshnikova E. A. Bakhlykova A. V. Zykova K. V. Romanchenko
author_facet	M. V. Nikolenko N. V. Baryshnikova E. A. Bakhlykova A. V. Zykova K. V. Romanchenko
author_sort	M. V. Nikolenko
title	Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
title_short	Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
title_full	Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
title_fullStr	Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
title_full_unstemmed	Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts
title_sort	bioregulation of amplitude-phase biological activity of candida albicans by women reproductive tract microsymbionts
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/5cf1c99c32b740f9b90876940b514a76
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Bioregulation of amplitude-phase biological activity of Candida albicans by women reproductive tract microsymbionts

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