Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.

Phytoplankton growth rates are limited by the supply of iron (Fe) in approximately one third of the open ocean, with major implications for carbon dioxide sequestration and carbon (C) biogeochemistry. To date, understanding how alteration of Fe supply changes phytoplankton physiology has focused on...

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Autores principales:	Brook L Nunn, Jessica F Faux, Anna A Hippmann, Maria T Maldonado, H Rodger Harvey, David R Goodlett, Philip W Boyd, Robert F Strzepek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cf6eb061b91420f93c56bc74bc07177
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spelling	oai:doaj.org-article:5cf6eb061b91420f93c56bc74bc071772021-11-18T08:50:53ZDiatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.1932-620310.1371/journal.pone.0075653https://doaj.org/article/5cf6eb061b91420f93c56bc74bc071772013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146769/?tool=EBIhttps://doaj.org/toc/1932-6203Phytoplankton growth rates are limited by the supply of iron (Fe) in approximately one third of the open ocean, with major implications for carbon dioxide sequestration and carbon (C) biogeochemistry. To date, understanding how alteration of Fe supply changes phytoplankton physiology has focused on traditional metrics such as growth rate, elemental composition, and biophysical measurements such as photosynthetic competence (Fv/Fm). Researchers have subsequently employed transcriptomics to probe relationships between changes in Fe supply and phytoplankton physiology. Recently, studies have investigated longer-term (i.e. following acclimation) responses of phytoplankton to various Fe conditions. In the present study, the coastal diatom, Thalassiosira pseudonana, was acclimated (10 generations) to either low or high Fe conditions, i.e. Fe-limiting and Fe-replete. Quantitative proteomics and a newly developed proteomic profiling technique that identifies low abundance proteins were employed to examine the full complement of expressed proteins and consequently the metabolic pathways utilized by the diatom under the two Fe conditions. A total of 1850 proteins were confidently identified, nearly tripling previous identifications made from differential expression in diatoms. Given sufficient time to acclimate to Fe limitation, T. pseudonana up-regulates proteins involved in pathways associated with intracellular protein recycling, thereby decreasing dependence on extracellular nitrogen (N), C and Fe. The relative increase in the abundance of photorespiration and pentose phosphate pathway proteins reveal novel metabolic shifts, which create substrates that could support other well-established physiological responses, such as heavily silicified frustules observed for Fe-limited diatoms. Here, we discovered that proteins and hence pathways observed to be down-regulated in short-term Fe starvation studies are constitutively expressed when T. pseudonana is acclimated (i.e., nitrate and nitrite transporters, Photosystem II and Photosystem I complexes). Acclimation of the diatom to the desired Fe conditions and the comprehensive proteomic approach provides a more robust interpretation of this dynamic proteome than previous studies.Brook L NunnJessica F FauxAnna A HippmannMaria T MaldonadoH Rodger HarveyDavid R GoodlettPhilip W BoydRobert F StrzepekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e75653 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brook L Nunn Jessica F Faux Anna A Hippmann Maria T Maldonado H Rodger Harvey David R Goodlett Philip W Boyd Robert F Strzepek Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.
description	Phytoplankton growth rates are limited by the supply of iron (Fe) in approximately one third of the open ocean, with major implications for carbon dioxide sequestration and carbon (C) biogeochemistry. To date, understanding how alteration of Fe supply changes phytoplankton physiology has focused on traditional metrics such as growth rate, elemental composition, and biophysical measurements such as photosynthetic competence (Fv/Fm). Researchers have subsequently employed transcriptomics to probe relationships between changes in Fe supply and phytoplankton physiology. Recently, studies have investigated longer-term (i.e. following acclimation) responses of phytoplankton to various Fe conditions. In the present study, the coastal diatom, Thalassiosira pseudonana, was acclimated (10 generations) to either low or high Fe conditions, i.e. Fe-limiting and Fe-replete. Quantitative proteomics and a newly developed proteomic profiling technique that identifies low abundance proteins were employed to examine the full complement of expressed proteins and consequently the metabolic pathways utilized by the diatom under the two Fe conditions. A total of 1850 proteins were confidently identified, nearly tripling previous identifications made from differential expression in diatoms. Given sufficient time to acclimate to Fe limitation, T. pseudonana up-regulates proteins involved in pathways associated with intracellular protein recycling, thereby decreasing dependence on extracellular nitrogen (N), C and Fe. The relative increase in the abundance of photorespiration and pentose phosphate pathway proteins reveal novel metabolic shifts, which create substrates that could support other well-established physiological responses, such as heavily silicified frustules observed for Fe-limited diatoms. Here, we discovered that proteins and hence pathways observed to be down-regulated in short-term Fe starvation studies are constitutively expressed when T. pseudonana is acclimated (i.e., nitrate and nitrite transporters, Photosystem II and Photosystem I complexes). Acclimation of the diatom to the desired Fe conditions and the comprehensive proteomic approach provides a more robust interpretation of this dynamic proteome than previous studies.
format	article
author	Brook L Nunn Jessica F Faux Anna A Hippmann Maria T Maldonado H Rodger Harvey David R Goodlett Philip W Boyd Robert F Strzepek
author_facet	Brook L Nunn Jessica F Faux Anna A Hippmann Maria T Maldonado H Rodger Harvey David R Goodlett Philip W Boyd Robert F Strzepek
author_sort	Brook L Nunn
title	Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.
title_short	Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.
title_full	Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.
title_fullStr	Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.
title_full_unstemmed	Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.
title_sort	diatom proteomics reveals unique acclimation strategies to mitigate fe limitation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5cf6eb061b91420f93c56bc74bc07177
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Diatom proteomics reveals unique acclimation strategies to mitigate Fe limitation.

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