Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration

Abstract Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs...

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Autores principales:	Kelly Cristine Santos Roballo, Juliano Coelho da Silveira, Fabiana Fernandes Bressan, Aline Fernanda de Souza, Vitoria Mattos Pereira, Jorge Eliecer Pinzon Porras, Felipe Augusto Rós, Lidia Hildebrand Pulz, Ricardo de Francisco Strefezzi, Daniele dos Santos Martins, Flavio Vieira Meirelles, Carlos Eduardo Ambrósio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5cf84b9f25c34d319497d9181ddefd66
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spelling	oai:doaj.org-article:5cf84b9f25c34d319497d9181ddefd662021-12-02T15:08:31ZNeurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration10.1038/s41598-019-47229-x2045-2322https://doaj.org/article/5cf84b9f25c34d319497d9181ddefd662019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47229-xhttps://doaj.org/toc/2045-2322Abstract Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury.Kelly Cristine Santos RoballoJuliano Coelho da SilveiraFabiana Fernandes BressanAline Fernanda de SouzaVitoria Mattos PereiraJorge Eliecer Pinzon PorrasFelipe Augusto RósLidia Hildebrand PulzRicardo de Francisco StrefezziDaniele dos Santos MartinsFlavio Vieira MeirellesCarlos Eduardo AmbrósioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kelly Cristine Santos Roballo Juliano Coelho da Silveira Fabiana Fernandes Bressan Aline Fernanda de Souza Vitoria Mattos Pereira Jorge Eliecer Pinzon Porras Felipe Augusto Rós Lidia Hildebrand Pulz Ricardo de Francisco Strefezzi Daniele dos Santos Martins Flavio Vieira Meirelles Carlos Eduardo Ambrósio Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
description	Abstract Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury.
format	article
author	Kelly Cristine Santos Roballo Juliano Coelho da Silveira Fabiana Fernandes Bressan Aline Fernanda de Souza Vitoria Mattos Pereira Jorge Eliecer Pinzon Porras Felipe Augusto Rós Lidia Hildebrand Pulz Ricardo de Francisco Strefezzi Daniele dos Santos Martins Flavio Vieira Meirelles Carlos Eduardo Ambrósio
author_facet	Kelly Cristine Santos Roballo Juliano Coelho da Silveira Fabiana Fernandes Bressan Aline Fernanda de Souza Vitoria Mattos Pereira Jorge Eliecer Pinzon Porras Felipe Augusto Rós Lidia Hildebrand Pulz Ricardo de Francisco Strefezzi Daniele dos Santos Martins Flavio Vieira Meirelles Carlos Eduardo Ambrósio
author_sort	Kelly Cristine Santos Roballo
title	Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_short	Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_full	Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_fullStr	Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_full_unstemmed	Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_sort	neurons-derived extracellular vesicles promote neural differentiation of adscs: a model to prevent peripheral nerve degeneration
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5cf84b9f25c34d319497d9181ddefd66
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Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration

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