Revisiting the Potential Functionality of the MagR Protein

Recent findings have sparked great interest in the putative magnetic receptor protein MagR. However, in vivo experiments have revealed no magnetic moment of MagR at room temperature. Nevertheless, the interaction of MagR and MagR fusion proteins with silica-coated magnetite beads have proven useful...

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Autores principales:	Alexander Pekarsky, Herwig Michor, Oliver Spadiut
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	magnetic receptor protein (MagR) <i>Escherichia coli</i> magnetism affinity chromatography SQUID Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/5cfd62c6942e48949112b5406aadfb16
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spelling	oai:doaj.org-article:5cfd62c6942e48949112b5406aadfb162021-11-25T18:12:31ZRevisiting the Potential Functionality of the MagR Protein10.3390/magnetochemistry71101472312-7481https://doaj.org/article/5cfd62c6942e48949112b5406aadfb162021-11-01T00:00:00Zhttps://www.mdpi.com/2312-7481/7/11/147https://doaj.org/toc/2312-7481Recent findings have sparked great interest in the putative magnetic receptor protein MagR. However, in vivo experiments have revealed no magnetic moment of MagR at room temperature. Nevertheless, the interaction of MagR and MagR fusion proteins with silica-coated magnetite beads have proven useful for protein purification. In this study, we recombinantly produced two different MagR proteins in <i>Escherichia coli</i> BL21(DE3) to (1) expand earlier protein purification studies, (2) test if MagR can magnetize whole <i>E. coli</i> cells once it is expressed to a high cytosolic, soluble titer, and (3) investigate the MagR-expressing <i>E. coli</i> cells’ magnetic properties at low temperatures. Our results show that MagR induces no measurable, permanent magnetic moment in cells at low temperatures, indicating no usability for cell magnetization. Furthermore, we show the limited usability for magnetic bead-based protein purification, thus closing the current knowledge gap between theoretical considerations and empirical data on the MagR protein.Alexander PekarskyHerwig MichorOliver SpadiutMDPI AGarticlemagnetic receptor protein (MagR)<i>Escherichia coli</i>magnetismaffinity chromatographySQUIDChemistryQD1-999ENMagnetochemistry, Vol 7, Iss 147, p 147 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic receptor protein (MagR) <i>Escherichia coli</i> magnetism affinity chromatography SQUID Chemistry QD1-999
spellingShingle	magnetic receptor protein (MagR) <i>Escherichia coli</i> magnetism affinity chromatography SQUID Chemistry QD1-999 Alexander Pekarsky Herwig Michor Oliver Spadiut Revisiting the Potential Functionality of the MagR Protein
description	Recent findings have sparked great interest in the putative magnetic receptor protein MagR. However, in vivo experiments have revealed no magnetic moment of MagR at room temperature. Nevertheless, the interaction of MagR and MagR fusion proteins with silica-coated magnetite beads have proven useful for protein purification. In this study, we recombinantly produced two different MagR proteins in <i>Escherichia coli</i> BL21(DE3) to (1) expand earlier protein purification studies, (2) test if MagR can magnetize whole <i>E. coli</i> cells once it is expressed to a high cytosolic, soluble titer, and (3) investigate the MagR-expressing <i>E. coli</i> cells’ magnetic properties at low temperatures. Our results show that MagR induces no measurable, permanent magnetic moment in cells at low temperatures, indicating no usability for cell magnetization. Furthermore, we show the limited usability for magnetic bead-based protein purification, thus closing the current knowledge gap between theoretical considerations and empirical data on the MagR protein.
format	article
author	Alexander Pekarsky Herwig Michor Oliver Spadiut
author_facet	Alexander Pekarsky Herwig Michor Oliver Spadiut
author_sort	Alexander Pekarsky
title	Revisiting the Potential Functionality of the MagR Protein
title_short	Revisiting the Potential Functionality of the MagR Protein
title_full	Revisiting the Potential Functionality of the MagR Protein
title_fullStr	Revisiting the Potential Functionality of the MagR Protein
title_full_unstemmed	Revisiting the Potential Functionality of the MagR Protein
title_sort	revisiting the potential functionality of the magr protein
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5cfd62c6942e48949112b5406aadfb16
work_keys_str_mv	AT alexanderpekarsky revisitingthepotentialfunctionalityofthemagrprotein AT herwigmichor revisitingthepotentialfunctionalityofthemagrprotein AT oliverspadiut revisitingthepotentialfunctionalityofthemagrprotein
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Revisiting the Potential Functionality of the MagR Protein

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