Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites

Hussam I Kutbi,1 Ahmed K Kammoun,2 Dalia Farag El-Telbany3 1Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department...

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Autores principales:	Kutbi HI, Kammoun AK, Farag El-Telbany D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	breast cancer zein pterostilbene nanoparticles apoptosis cell cycle oxidative stress Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5d1b082f1689456fbf2eb3751272eade
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spelling	oai:doaj.org-article:5d1b082f1689456fbf2eb3751272eade2021-12-02T15:39:41ZAmelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites1178-2013https://doaj.org/article/5d1b082f1689456fbf2eb3751272eade2021-04-01T00:00:00Zhttps://www.dovepress.com/amelioration-of-pterostilbene-antiproliferative-proapoptotic-and-oxida-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Hussam I Kutbi,1 Ahmed K Kammoun,2 Dalia Farag El-Telbany3 1Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department of Pharmaceutics, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo, 11571, EgyptCorrespondence: Dalia Farag El-TelbanyDepartment of Pharmaceutics, Faculty of Pharmacy, Modern University for Technology and Information (MTI) University, El-Hadaba El-Wosta, 5th District, Mokatam, Cairo, 11571, EgyptTel +20-11-1996-6617Fax +20227294500Email dalia.altelbany@gmail.comPurpose: This study aimed to explain the influence of zein nanosphere (ZN NS) formulation on the pharmacotherapeutic profile of PTS in MCF7 cells.Methods: Liquid–liquid phase separation was used to formulate PTS-ZN NSs. The formulations developed were evaluated for particle-size analysis, encapsulation efficiency, and in vitro diffusion. Also, assays of cytotoxicity, uptake, cell-cycle progression, annexin V, apoptotic gene mRNA expression and biochemical assays were carried out.Results: The PTS-ZN NS formulation selected showed 104.5± 6.2 nm, 33.4± 1.8 mV, 95.1%± 3.6%, and 89.1%± 2.65% average particle size, zeta-potential, encapsulation efficiency and in vitro diffusion, respectively. With MCF7 cells, IC50 was reduced approximately 15-fold, with increased cellular uptake, accumulation in the G2/M phase, increased percentage of cells in the pre-G1 phase, amelioration of early and late apoptosis, raised mRNA expression of CASP3 and CASP7, lower expression of cyclin-CDK1, and enhanced oxidant potential through decreased glutathione reductase (GR) activity, and enhanced reactive oxygen–species generation and lipid-peroxidation products.Conclusion: PTS-ZN NSs indicated enhanced antiproliferative, proapoptotic, and oxidant potential toward MCF7 cells compared to free PTS. Ameliorated results of nanosized carriers, cellular uptake, and sustained diffusion may contribute to these outcomes.Keywords: phototherapy, pterostilbene, MCF7 cells, nanospheres, zeinKutbi HIKammoun AKFarag El-Telbany DDove Medical Pressarticlebreast cancerzeinpterostilbenenanoparticlesapoptosiscell cycleoxidative stressMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3059-3071 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer zein pterostilbene nanoparticles apoptosis cell cycle oxidative stress Medicine (General) R5-920
spellingShingle	breast cancer zein pterostilbene nanoparticles apoptosis cell cycle oxidative stress Medicine (General) R5-920 Kutbi HI Kammoun AK Farag El-Telbany D Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites
description	Hussam I Kutbi,1 Ahmed K Kammoun,2 Dalia Farag El-Telbany3 1Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department of Pharmaceutics, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo, 11571, EgyptCorrespondence: Dalia Farag El-TelbanyDepartment of Pharmaceutics, Faculty of Pharmacy, Modern University for Technology and Information (MTI) University, El-Hadaba El-Wosta, 5th District, Mokatam, Cairo, 11571, EgyptTel +20-11-1996-6617Fax +20227294500Email dalia.altelbany@gmail.comPurpose: This study aimed to explain the influence of zein nanosphere (ZN NS) formulation on the pharmacotherapeutic profile of PTS in MCF7 cells.Methods: Liquid–liquid phase separation was used to formulate PTS-ZN NSs. The formulations developed were evaluated for particle-size analysis, encapsulation efficiency, and in vitro diffusion. Also, assays of cytotoxicity, uptake, cell-cycle progression, annexin V, apoptotic gene mRNA expression and biochemical assays were carried out.Results: The PTS-ZN NS formulation selected showed 104.5± 6.2 nm, 33.4± 1.8 mV, 95.1%± 3.6%, and 89.1%± 2.65% average particle size, zeta-potential, encapsulation efficiency and in vitro diffusion, respectively. With MCF7 cells, IC50 was reduced approximately 15-fold, with increased cellular uptake, accumulation in the G2/M phase, increased percentage of cells in the pre-G1 phase, amelioration of early and late apoptosis, raised mRNA expression of CASP3 and CASP7, lower expression of cyclin-CDK1, and enhanced oxidant potential through decreased glutathione reductase (GR) activity, and enhanced reactive oxygen–species generation and lipid-peroxidation products.Conclusion: PTS-ZN NSs indicated enhanced antiproliferative, proapoptotic, and oxidant potential toward MCF7 cells compared to free PTS. Ameliorated results of nanosized carriers, cellular uptake, and sustained diffusion may contribute to these outcomes.Keywords: phototherapy, pterostilbene, MCF7 cells, nanospheres, zein
format	article
author	Kutbi HI Kammoun AK Farag El-Telbany D
author_facet	Kutbi HI Kammoun AK Farag El-Telbany D
author_sort	Kutbi HI
title	Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites
title_short	Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites
title_full	Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites
title_fullStr	Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites
title_full_unstemmed	Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites
title_sort	amelioration of pterostilbene antiproliferative, proapoptotic, and oxidant potentials in human breast cancer mcf7 cells using zein nanocomposites
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/5d1b082f1689456fbf2eb3751272eade
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Amelioration of Pterostilbene Antiproliferative, Proapoptotic, and Oxidant Potentials in Human Breast Cancer MCF7 Cells Using Zein Nanocomposites

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