PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage

Abstract Intermittent Parathyroid Hormone (I-PTH) is the only FDA approved anabolic drug therapy available for the treatment of osteoporosis in males and postmenopausal females. The effects of I-PTH on the chondrogenic lineage of the mandibular condylar cartilage (MCC) are not well understood. To in...

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Autores principales:	Mara Heather O’ Brien, Eliane Hermes Dutra, Alexandro Lima, Ravindra Nanda, Sumit Yadav
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d214f32d93e407d888ed9bdd3563f11
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spelling	oai:doaj.org-article:5d214f32d93e407d888ed9bdd3563f112021-12-02T12:32:34ZPTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage10.1038/s41598-017-03428-y2045-2322https://doaj.org/article/5d214f32d93e407d888ed9bdd3563f112017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03428-yhttps://doaj.org/toc/2045-2322Abstract Intermittent Parathyroid Hormone (I-PTH) is the only FDA approved anabolic drug therapy available for the treatment of osteoporosis in males and postmenopausal females. The effects of I-PTH on the chondrogenic lineage of the mandibular condylar cartilage (MCC) are not well understood. To investigate the role of I-PTH on the MCC and subchondral bone, we carried out our studies using 4 to 5 week old triple transgenic mice (Col1a1XCol2a1XCol10a1). The experimental group was injected with PTH (80 μg/kg) daily for 2 weeks, while control group was injected with saline. Our histology showed that the I-PTH treatment led to an increased number of cells expressing Col1a1, Col2a1 and Col10a1. Additionally, there was an increase in cellular proliferation, increased proteoglycan distribution, increased cartilage thickness, increased TRAP activity, and mineralization. Immunohistochemical staining showed increased expression of pSMAD158 and VEGF in the MCC and subchondral bone. Furthermore our microCT data showed that I-PTH treatment led to an increased bone volume fraction, tissue density and trabecular thickness, with a decrease in trabecular spacing. Morphometric measurements showed increased mandibular length and condyle head length following I-PTH treatment. In conclusion, our study suggests that I-PTH plays a critical role in cellular proliferation, proteoglycan distribution, and mineralization of the MCC.Mara Heather O’ BrienEliane Hermes DutraAlexandro LimaRavindra NandaSumit YadavNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mara Heather O’ Brien Eliane Hermes Dutra Alexandro Lima Ravindra Nanda Sumit Yadav PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage
description	Abstract Intermittent Parathyroid Hormone (I-PTH) is the only FDA approved anabolic drug therapy available for the treatment of osteoporosis in males and postmenopausal females. The effects of I-PTH on the chondrogenic lineage of the mandibular condylar cartilage (MCC) are not well understood. To investigate the role of I-PTH on the MCC and subchondral bone, we carried out our studies using 4 to 5 week old triple transgenic mice (Col1a1XCol2a1XCol10a1). The experimental group was injected with PTH (80 μg/kg) daily for 2 weeks, while control group was injected with saline. Our histology showed that the I-PTH treatment led to an increased number of cells expressing Col1a1, Col2a1 and Col10a1. Additionally, there was an increase in cellular proliferation, increased proteoglycan distribution, increased cartilage thickness, increased TRAP activity, and mineralization. Immunohistochemical staining showed increased expression of pSMAD158 and VEGF in the MCC and subchondral bone. Furthermore our microCT data showed that I-PTH treatment led to an increased bone volume fraction, tissue density and trabecular thickness, with a decrease in trabecular spacing. Morphometric measurements showed increased mandibular length and condyle head length following I-PTH treatment. In conclusion, our study suggests that I-PTH plays a critical role in cellular proliferation, proteoglycan distribution, and mineralization of the MCC.
format	article
author	Mara Heather O’ Brien Eliane Hermes Dutra Alexandro Lima Ravindra Nanda Sumit Yadav
author_facet	Mara Heather O’ Brien Eliane Hermes Dutra Alexandro Lima Ravindra Nanda Sumit Yadav
author_sort	Mara Heather O’ Brien
title	PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage
title_short	PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage
title_full	PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage
title_fullStr	PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage
title_full_unstemmed	PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage
title_sort	pth [1–34] induced differentiation and mineralization of mandibular condylar cartilage
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5d214f32d93e407d888ed9bdd3563f11
work_keys_str_mv	AT maraheatherobrien pth134induceddifferentiationandmineralizationofmandibularcondylarcartilage AT elianehermesdutra pth134induceddifferentiationandmineralizationofmandibularcondylarcartilage AT alexandrolima pth134induceddifferentiationandmineralizationofmandibularcondylarcartilage AT ravindrananda pth134induceddifferentiationandmineralizationofmandibularcondylarcartilage AT sumityadav pth134induceddifferentiationandmineralizationofmandibularcondylarcartilage
_version_	1718394037034024960

PTH [1–34] induced differentiation and mineralization of mandibular condylar cartilage

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