Sniffer cells for the detection of neural Angiotensin II in vitro

Abstract Neuropeptide release in the brain has traditionally been difficult to observe. Existing methods lack temporal and spatial resolution that is consistent with the function and size of neurons. We use cultured “sniffer cells” to improve the temporal and spatial resolution of observing neuropep...

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Autores principales:	George E. Farmer, Anna Amune, Martha E. Bachelor, Phong Duong, Joseph P. Yuan, J. Thomas Cunningham
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d31e5e9234347df89331ea08aac6961
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spelling	oai:doaj.org-article:5d31e5e9234347df89331ea08aac69612021-12-02T15:07:55ZSniffer cells for the detection of neural Angiotensin II in vitro10.1038/s41598-019-45262-42045-2322https://doaj.org/article/5d31e5e9234347df89331ea08aac69612019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45262-4https://doaj.org/toc/2045-2322Abstract Neuropeptide release in the brain has traditionally been difficult to observe. Existing methods lack temporal and spatial resolution that is consistent with the function and size of neurons. We use cultured “sniffer cells” to improve the temporal and spatial resolution of observing neuropeptide release. Sniffer cells were created by stably transfecting Chinese Hamster Ovary (CHO) cells with plasmids encoding the rat angiotensin type 1a receptor and a genetically encoded Ca2+ sensor. Isolated, cultured sniffer cells showed dose-dependent increases in fluorescence in response to exogenously applied angiotensin II and III, but not other common neurotransmitters. Sniffer cells placed on the median preoptic nucleus (a presumptive site of angiotensin release) displayed spontaneous activity and evoked responses to either electrical or optogenetic stimulation of the subfornical organ. Stable sniffer cell lines could be a viable method for detecting neuropeptide release in vitro, while still being able to distinguish differences in neuropeptide concentration.George E. FarmerAnna AmuneMartha E. BachelorPhong DuongJoseph P. YuanJ. Thomas CunninghamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q George E. Farmer Anna Amune Martha E. Bachelor Phong Duong Joseph P. Yuan J. Thomas Cunningham Sniffer cells for the detection of neural Angiotensin II in vitro
description	Abstract Neuropeptide release in the brain has traditionally been difficult to observe. Existing methods lack temporal and spatial resolution that is consistent with the function and size of neurons. We use cultured “sniffer cells” to improve the temporal and spatial resolution of observing neuropeptide release. Sniffer cells were created by stably transfecting Chinese Hamster Ovary (CHO) cells with plasmids encoding the rat angiotensin type 1a receptor and a genetically encoded Ca2+ sensor. Isolated, cultured sniffer cells showed dose-dependent increases in fluorescence in response to exogenously applied angiotensin II and III, but not other common neurotransmitters. Sniffer cells placed on the median preoptic nucleus (a presumptive site of angiotensin release) displayed spontaneous activity and evoked responses to either electrical or optogenetic stimulation of the subfornical organ. Stable sniffer cell lines could be a viable method for detecting neuropeptide release in vitro, while still being able to distinguish differences in neuropeptide concentration.
format	article
author	George E. Farmer Anna Amune Martha E. Bachelor Phong Duong Joseph P. Yuan J. Thomas Cunningham
author_facet	George E. Farmer Anna Amune Martha E. Bachelor Phong Duong Joseph P. Yuan J. Thomas Cunningham
author_sort	George E. Farmer
title	Sniffer cells for the detection of neural Angiotensin II in vitro
title_short	Sniffer cells for the detection of neural Angiotensin II in vitro
title_full	Sniffer cells for the detection of neural Angiotensin II in vitro
title_fullStr	Sniffer cells for the detection of neural Angiotensin II in vitro
title_full_unstemmed	Sniffer cells for the detection of neural Angiotensin II in vitro
title_sort	sniffer cells for the detection of neural angiotensin ii in vitro
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5d31e5e9234347df89331ea08aac6961
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Sniffer cells for the detection of neural Angiotensin II in vitro

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