Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine

Abstract Conjugate vaccine platform is a promising strategy to overcome the poor immunogenicity of bacterial polysaccharide antigens in infants and children. A carrier protein in conjugate vaccines works not only as an immune stimulator to polysaccharide, but also as an immunogen; with the latter ge...

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Autores principales:	Wook-Jin Park, Yeon-Kyung Yoon, Ji-Sun Park, Ruchirkumar Pansuriya, Yeong-Jae Seok, Ravi Ganapathy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d37cb8763e54effbd1aef2e4478cbac
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spelling	oai:doaj.org-article:5d37cb8763e54effbd1aef2e4478cbac2021-11-14T12:18:54ZRotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine10.1038/s41598-021-01549-z2045-2322https://doaj.org/article/5d37cb8763e54effbd1aef2e4478cbac2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01549-zhttps://doaj.org/toc/2045-2322Abstract Conjugate vaccine platform is a promising strategy to overcome the poor immunogenicity of bacterial polysaccharide antigens in infants and children. A carrier protein in conjugate vaccines works not only as an immune stimulator to polysaccharide, but also as an immunogen; with the latter generally not considered as a measured outcome in real world. Here, we probed the potential of a conjugate vaccine platform to induce enhanced immunogenicity of a truncated rotavirus spike protein ΔVP8. ΔVP8 was covalently conjugated to Vi capsular polysaccharide (Vi) of Salmonella Typhi to develop a bivalent vaccine, termed Vi-ΔVP8. Our results demonstrated that the Vi-ΔVP8 vaccine can induce specific immune responses against both antigens in immunized mice. The conjugate vaccine elicits high antibody titers and functional antibodies against S. Typhi and Rotavirus (RV) when compared to immunization with a single antigen. Together, these results indicate that Vi-ΔVP8* is a potent and immunogenic vaccine candidate, thus strengthening the potential of conjugate vaccine platform with enhanced immune responses to carrier protein, including ΔVP8*.Wook-Jin ParkYeon-Kyung YoonJi-Sun ParkRuchirkumar PansuriyaYeong-Jae SeokRavi GanapathyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wook-Jin Park Yeon-Kyung Yoon Ji-Sun Park Ruchirkumar Pansuriya Yeong-Jae Seok Ravi Ganapathy Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
description	Abstract Conjugate vaccine platform is a promising strategy to overcome the poor immunogenicity of bacterial polysaccharide antigens in infants and children. A carrier protein in conjugate vaccines works not only as an immune stimulator to polysaccharide, but also as an immunogen; with the latter generally not considered as a measured outcome in real world. Here, we probed the potential of a conjugate vaccine platform to induce enhanced immunogenicity of a truncated rotavirus spike protein ΔVP8. ΔVP8 was covalently conjugated to Vi capsular polysaccharide (Vi) of Salmonella Typhi to develop a bivalent vaccine, termed Vi-ΔVP8. Our results demonstrated that the Vi-ΔVP8 vaccine can induce specific immune responses against both antigens in immunized mice. The conjugate vaccine elicits high antibody titers and functional antibodies against S. Typhi and Rotavirus (RV) when compared to immunization with a single antigen. Together, these results indicate that Vi-ΔVP8* is a potent and immunogenic vaccine candidate, thus strengthening the potential of conjugate vaccine platform with enhanced immune responses to carrier protein, including ΔVP8*.
format	article
author	Wook-Jin Park Yeon-Kyung Yoon Ji-Sun Park Ruchirkumar Pansuriya Yeong-Jae Seok Ravi Ganapathy
author_facet	Wook-Jin Park Yeon-Kyung Yoon Ji-Sun Park Ruchirkumar Pansuriya Yeong-Jae Seok Ravi Ganapathy
author_sort	Wook-Jin Park
title	Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
title_short	Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
title_full	Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
title_fullStr	Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
title_full_unstemmed	Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
title_sort	rotavirus spike protein δvp8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5d37cb8763e54effbd1aef2e4478cbac
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Rotavirus spike protein ΔVP8* as a novel carrier protein for conjugate vaccine platform with demonstrated antigenic potential for use as bivalent vaccine

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