High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.

The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thomas J F Nieland, David J Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E Root, Anne E Carpenter, Bernardo L Sabatini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d3bee3942524ddeb31a3659de87e7cb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5d3bee3942524ddeb31a3659de87e7cb
record_format	dspace
spelling	oai:doaj.org-article:5d3bee3942524ddeb31a3659de87e7cb2021-11-18T08:27:56ZHigh content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.1932-620310.1371/journal.pone.0091744https://doaj.org/article/5d3bee3942524ddeb31a3659de87e7cb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24633176/?tool=EBIhttps://doaj.org/toc/1932-6203The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders.Thomas J F NielandDavid J LoganJessica SaulnierDaniel LamCaroline JohnsonDavid E RootAnne E CarpenterBernardo L SabatiniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91744 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas J F Nieland David J Logan Jessica Saulnier Daniel Lam Caroline Johnson David E Root Anne E Carpenter Bernardo L Sabatini High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
description	The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders.
format	article
author	Thomas J F Nieland David J Logan Jessica Saulnier Daniel Lam Caroline Johnson David E Root Anne E Carpenter Bernardo L Sabatini
author_facet	Thomas J F Nieland David J Logan Jessica Saulnier Daniel Lam Caroline Johnson David E Root Anne E Carpenter Bernardo L Sabatini
author_sort	Thomas J F Nieland
title	High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
title_short	High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
title_full	High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
title_fullStr	High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
title_full_unstemmed	High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
title_sort	high content image analysis identifies novel regulators of synaptogenesis in a high-throughput rnai screen of primary neurons.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5d3bee3942524ddeb31a3659de87e7cb
work_keys_str_mv	AT thomasjfnieland highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT davidjlogan highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT jessicasaulnier highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT daniellam highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT carolinejohnson highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT davideroot highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT anneecarpenter highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons AT bernardolsabatini highcontentimageanalysisidentifiesnovelregulatorsofsynaptogenesisinahighthroughputrnaiscreenofprimaryneurons
_version_	1718421742553137152

High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.

Ejemplares similares