Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC

Phenolic compounds of plants have a wide spectrum of pharmacological action. A number of effective herbal medicines and biologically active additives have been developed on the basis of raw materials and natural polyphenols. The sources of phenolic compounds are plants of the genus Bupleurum L. In R...

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Autores principales: V. M. Mirovich, S. A. Petukhova, L. V. Dudareva
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Publicado: Scientific Сentre for Family Health and Human Reproduction Problems 2017
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spelling oai:doaj.org-article:5d415252adeb4e0e86756cc79bc34f012021-11-23T06:14:37ZStudying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC2541-94202587-959610.12737/article_59f035fe65fc57.52277900https://doaj.org/article/5d415252adeb4e0e86756cc79bc34f012017-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/394https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Phenolic compounds of plants have a wide spectrum of pharmacological action. A number of effective herbal medicines and biologically active additives have been developed on the basis of raw materials and natural polyphenols. The sources of phenolic compounds are plants of the genus Bupleurum L. In Russia Bupleurum scorzonerifolium used in folk medicine. Infusions and decoctions were taken for diseases of the liver, gall bladder, topically as an anti-inflammatory agent. The composition of phenolic compounds in the above-ground organs of the B. scorzonerifolium studied by HPLC on the device "GILSTON". Samples of raw materials (above-ground organs) were collected in the southern areas of the Irkutsk region. For the analysis of the herb B. scorzonerifolium 70% extract ethyl alcohol was used. Separation was performed on substances Kromasil C18 column (4,6 x 250 mm, 5 micron). Detection was carried out using a UV detector "GILSTON" UV/VIS model 151, at a wavelength of 254 nm. In comparison with standard samples we identified 8 flavonoids (prevailing - izoquercetin, hyperoside, izoramnetin-3-glucoside), 5 aromatic acids (predominantly acid - cinnamic, gallic, chicory). The study established the content of epicatechin, catechin, epigallocatechingallate, coumarin. For the first time the content of acids - coffee, chlorogenic, gallic, chicory, cinnamon; flavonoids - vitsenin, lyuteolin-7-glucoside, isoramnetin-3-glucoside, apigenin; epicatechin, catechin, epigallocatechingallate; coumarin -was established in B. scorzonerifolium.V. M. MirovichS. A. PetukhovaL. V. DudarevaScientific Сentre for Family Health and Human Reproduction Problemsarticlebupleurum scorzonerifolium willdhplcphenol compoundsflavonoidstanning agentsphenolcarbonic acidsbupleurum scorzonerifolium willdScienceQRUActa Biomedica Scientifica, Vol 2, Iss 3, Pp 75-77 (2017)
institution DOAJ
collection DOAJ
language RU
topic bupleurum scorzonerifolium willd
hplc
phenol compounds
flavonoids
tanning agents
phenolcarbonic acids
bupleurum scorzonerifolium willd
Science
Q
spellingShingle bupleurum scorzonerifolium willd
hplc
phenol compounds
flavonoids
tanning agents
phenolcarbonic acids
bupleurum scorzonerifolium willd
Science
Q
V. M. Mirovich
S. A. Petukhova
L. V. Dudareva
Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC
description Phenolic compounds of plants have a wide spectrum of pharmacological action. A number of effective herbal medicines and biologically active additives have been developed on the basis of raw materials and natural polyphenols. The sources of phenolic compounds are plants of the genus Bupleurum L. In Russia Bupleurum scorzonerifolium used in folk medicine. Infusions and decoctions were taken for diseases of the liver, gall bladder, topically as an anti-inflammatory agent. The composition of phenolic compounds in the above-ground organs of the B. scorzonerifolium studied by HPLC on the device "GILSTON". Samples of raw materials (above-ground organs) were collected in the southern areas of the Irkutsk region. For the analysis of the herb B. scorzonerifolium 70% extract ethyl alcohol was used. Separation was performed on substances Kromasil C18 column (4,6 x 250 mm, 5 micron). Detection was carried out using a UV detector "GILSTON" UV/VIS model 151, at a wavelength of 254 nm. In comparison with standard samples we identified 8 flavonoids (prevailing - izoquercetin, hyperoside, izoramnetin-3-glucoside), 5 aromatic acids (predominantly acid - cinnamic, gallic, chicory). The study established the content of epicatechin, catechin, epigallocatechingallate, coumarin. For the first time the content of acids - coffee, chlorogenic, gallic, chicory, cinnamon; flavonoids - vitsenin, lyuteolin-7-glucoside, isoramnetin-3-glucoside, apigenin; epicatechin, catechin, epigallocatechingallate; coumarin -was established in B. scorzonerifolium.
format article
author V. M. Mirovich
S. A. Petukhova
L. V. Dudareva
author_facet V. M. Mirovich
S. A. Petukhova
L. V. Dudareva
author_sort V. M. Mirovich
title Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC
title_short Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC
title_full Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC
title_fullStr Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC
title_full_unstemmed Studying the content of phenolic compounds of Bupleurum scorzonerifolium Willd., growing in the Baikal region with HPLC
title_sort studying the content of phenolic compounds of bupleurum scorzonerifolium willd., growing in the baikal region with hplc
publisher Scientific Сentre for Family Health and Human Reproduction Problems
publishDate 2017
url https://doaj.org/article/5d415252adeb4e0e86756cc79bc34f01
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