Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Bing Du,1,2 Weizhen Liu,1 Yue Deng,1,3 Shaobing Li,1 Xiangning Liu,4 Yan Gao,1 Lei Zhou1 1Department of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Center of Stomatology, The First People’...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Du B, Liu W, Deng Y, Li S, Liu X, Gao Y, Zhou L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5d459a312a05455797a6f0c55c23577e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5d459a312a05455797a6f0c55c23577e
record_format	dspace
spelling	oai:doaj.org-article:5d459a312a05455797a6f0c55c23577e2021-12-02T05:39:10ZAngiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo1178-2013https://doaj.org/article/5d459a312a05455797a6f0c55c23577e2015-03-01T00:00:00Zhttp://www.dovepress.com/angiogenesis-and-bone-regeneration-of-porous-nano-hydroxyapatitecorall-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Bing Du,1,2 Weizhen Liu,1 Yue Deng,1,3 Shaobing Li,1 Xiangning Liu,4 Yan Gao,1 Lei Zhou1 1Department of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Center of Stomatology, The First People’s Hospital of Foshan, Foshan, Guangdong, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, Qingdao Stomatological Hospital, Qingdao, People’s Republic of China; 4Department of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou, People’s Republic of China Abstract: To improve the regenerative performance of nano-hydroxyapatite/coralline (nHA/coral) block grafting in a canine mandibular critical-size defect model, nHA/coral blocks were coated with recombinant human vascular endothelial growth factor165 (rhVEGF) via physical adsorption (3 µg rhVEGF165 per nHA/coral block). After the nHA/coral blocks and VEGF/nHA/coral blocks were randomly implanted into the mandibular box-shaped defects in a split-mouth design, the healing process was evaluated by histological observation and histomorphometric and immunohistological analyses. The histological evaluations revealed the ingrowth of newly formed blood vessels and bone at the periphery and cores of the blocks in both groups at both 3 and 8 weeks postsurgery, respectively. In the histomorphometric analysis, the VEGF/nHA/coral group exhibited a larger quantity of new bone formation at 3 and 8 weeks postsurgery. The percentages of newly formed bone within the entire blocks in the VEGF/nHA/coral group were 27.3%±8.1% and 39.3%±12.8% at 3 weeks and 8 weeks, respectively, and these values were slightly greater than those of the nHA/coral group (21.7%±3.0% and 32.6%±10.3%, respectively), but the differences were not significant (P>0.05). The immunohistological evaluations revealed that the neovascular density in the VEGF/nHA/coral group (146±32.9 vessel/mm2) was much greater than that in the nHA/coral group (105±51.8 vessel/mm2) at the 3-week time point (P<0.05), but no significant difference was observed at the 8-week time point (341±86.1 and 269±50.7 vessel/mm2, respectively, P>0.05). The present study indicated that nHA/coral blocks might be optimal scaffolds for block grafting in critical-size mandibular defects and that additional VEGF coating via physical adsorption can promote angiogenesis in the early stage of bone healing, which suggests that prevascularized nHA/coral blocks have significant potential as a bioactive material for bone regeneration in large-scale alveolar defects. Keywords: angiogenesis, bone regeneration, tissue engineering, block grafting, nano-hydroxyapatite/coralline, critical size, bone defect Du BLiu WDeng YLi SLiu XGao YZhou LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2555-2565 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Du B Liu W Deng Y Li S Liu X Gao Y Zhou L Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo
description	Bing Du,1,2 Weizhen Liu,1 Yue Deng,1,3 Shaobing Li,1 Xiangning Liu,4 Yan Gao,1 Lei Zhou1 1Department of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Center of Stomatology, The First People’s Hospital of Foshan, Foshan, Guangdong, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, Qingdao Stomatological Hospital, Qingdao, People’s Republic of China; 4Department of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou, People’s Republic of China Abstract: To improve the regenerative performance of nano-hydroxyapatite/coralline (nHA/coral) block grafting in a canine mandibular critical-size defect model, nHA/coral blocks were coated with recombinant human vascular endothelial growth factor165 (rhVEGF) via physical adsorption (3 µg rhVEGF165 per nHA/coral block). After the nHA/coral blocks and VEGF/nHA/coral blocks were randomly implanted into the mandibular box-shaped defects in a split-mouth design, the healing process was evaluated by histological observation and histomorphometric and immunohistological analyses. The histological evaluations revealed the ingrowth of newly formed blood vessels and bone at the periphery and cores of the blocks in both groups at both 3 and 8 weeks postsurgery, respectively. In the histomorphometric analysis, the VEGF/nHA/coral group exhibited a larger quantity of new bone formation at 3 and 8 weeks postsurgery. The percentages of newly formed bone within the entire blocks in the VEGF/nHA/coral group were 27.3%±8.1% and 39.3%±12.8% at 3 weeks and 8 weeks, respectively, and these values were slightly greater than those of the nHA/coral group (21.7%±3.0% and 32.6%±10.3%, respectively), but the differences were not significant (P>0.05). The immunohistological evaluations revealed that the neovascular density in the VEGF/nHA/coral group (146±32.9 vessel/mm2) was much greater than that in the nHA/coral group (105±51.8 vessel/mm2) at the 3-week time point (P<0.05), but no significant difference was observed at the 8-week time point (341±86.1 and 269±50.7 vessel/mm2, respectively, P>0.05). The present study indicated that nHA/coral blocks might be optimal scaffolds for block grafting in critical-size mandibular defects and that additional VEGF coating via physical adsorption can promote angiogenesis in the early stage of bone healing, which suggests that prevascularized nHA/coral blocks have significant potential as a bioactive material for bone regeneration in large-scale alveolar defects. Keywords: angiogenesis, bone regeneration, tissue engineering, block grafting, nano-hydroxyapatite/coralline, critical size, bone defect
format	article
author	Du B Liu W Deng Y Li S Liu X Gao Y Zhou L
author_facet	Du B Liu W Deng Y Li S Liu X Gao Y Zhou L
author_sort	Du B
title	Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo
title_short	Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo
title_full	Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo
title_fullStr	Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo
title_full_unstemmed	Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo
title_sort	angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhvegf165 in critical-size alveolar bone defects in vivo
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/5d459a312a05455797a6f0c55c23577e
work_keys_str_mv	AT dub angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo AT liuw angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo AT dengy angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo AT lis angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo AT liux angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo AT gaoy angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo AT zhoul angiogenesisandboneregenerationofporousnanohydroxyapatitecorallineblockscoatedwithrhvegf165incriticalsizealveolarbonedefectsinvivo
_version_	1718400298177789952

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Ejemplares similares