Real-time measurement of volatile chemicals released by bed bugs during mating activities.

In recent years, bed bug (Hemiptera: Cimicidae) problems have increased dramatically in many parts of the world, leading to a renewed interest in their chemical ecology. Most studies of bed bug semiochemicals have been based on the collection of volatiles over a period of time followed by chemical a...

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Autores principales:	Ole Kilpinen, Dezhao Liu, Anders Peter S Adamsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d48d197cac74d63aaade0bf25f963e7
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spelling	oai:doaj.org-article:5d48d197cac74d63aaade0bf25f963e72021-11-18T08:06:15ZReal-time measurement of volatile chemicals released by bed bugs during mating activities.1932-620310.1371/journal.pone.0050981https://doaj.org/article/5d48d197cac74d63aaade0bf25f963e72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23227225/?tool=EBIhttps://doaj.org/toc/1932-6203In recent years, bed bug (Hemiptera: Cimicidae) problems have increased dramatically in many parts of the world, leading to a renewed interest in their chemical ecology. Most studies of bed bug semiochemicals have been based on the collection of volatiles over a period of time followed by chemical analysis. Here we present for the first time, a combination of proton transfer reaction mass spectrometry and video analysis for real-time measurement of semiochemicals emitted by isolated groups of bed bugs during specific behavioural activities. The most distinct peaks in the proton transfer reaction mass spectrometry recordings were always observed close to the termination of mating attempts, corresponding to the defensive emissions that bed bugs have been suspected to exploit for prevention of unwanted copulations. The main components of these emissions were (E)-2-hexenal and (E)-2-octenal recorded in ratios between 1:3 and 3:1. In the current study, the quantity varied over 1000 fold for both of the compounds with up to 40 µg total release in a single emission. Males also emit defensive compounds due to homosexual copulation attempts by other males, and no significant differences were observed in the ratio or the amount of the two components released from males or females. In summary, this study has demonstrated that combining proton-transfer-reaction mass spectrometry with video analysis can provide detailed information about semiochemicals emitted during specific behavioural activities.Ole KilpinenDezhao LiuAnders Peter S AdamsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e50981 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ole Kilpinen Dezhao Liu Anders Peter S Adamsen Real-time measurement of volatile chemicals released by bed bugs during mating activities.
description	In recent years, bed bug (Hemiptera: Cimicidae) problems have increased dramatically in many parts of the world, leading to a renewed interest in their chemical ecology. Most studies of bed bug semiochemicals have been based on the collection of volatiles over a period of time followed by chemical analysis. Here we present for the first time, a combination of proton transfer reaction mass spectrometry and video analysis for real-time measurement of semiochemicals emitted by isolated groups of bed bugs during specific behavioural activities. The most distinct peaks in the proton transfer reaction mass spectrometry recordings were always observed close to the termination of mating attempts, corresponding to the defensive emissions that bed bugs have been suspected to exploit for prevention of unwanted copulations. The main components of these emissions were (E)-2-hexenal and (E)-2-octenal recorded in ratios between 1:3 and 3:1. In the current study, the quantity varied over 1000 fold for both of the compounds with up to 40 µg total release in a single emission. Males also emit defensive compounds due to homosexual copulation attempts by other males, and no significant differences were observed in the ratio or the amount of the two components released from males or females. In summary, this study has demonstrated that combining proton-transfer-reaction mass spectrometry with video analysis can provide detailed information about semiochemicals emitted during specific behavioural activities.
format	article
author	Ole Kilpinen Dezhao Liu Anders Peter S Adamsen
author_facet	Ole Kilpinen Dezhao Liu Anders Peter S Adamsen
author_sort	Ole Kilpinen
title	Real-time measurement of volatile chemicals released by bed bugs during mating activities.
title_short	Real-time measurement of volatile chemicals released by bed bugs during mating activities.
title_full	Real-time measurement of volatile chemicals released by bed bugs during mating activities.
title_fullStr	Real-time measurement of volatile chemicals released by bed bugs during mating activities.
title_full_unstemmed	Real-time measurement of volatile chemicals released by bed bugs during mating activities.
title_sort	real-time measurement of volatile chemicals released by bed bugs during mating activities.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5d48d197cac74d63aaade0bf25f963e7
work_keys_str_mv	AT olekilpinen realtimemeasurementofvolatilechemicalsreleasedbybedbugsduringmatingactivities AT dezhaoliu realtimemeasurementofvolatilechemicalsreleasedbybedbugsduringmatingactivities AT anderspetersadamsen realtimemeasurementofvolatilechemicalsreleasedbybedbugsduringmatingactivities
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Real-time measurement of volatile chemicals released by bed bugs during mating activities.

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