Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients

Abstract There is a stringent need to find means for risk stratification of coronary artery diseases (CAD) patients. We aimed at identifying alterations of plasma high-density lipoproteins (HDL) components and their validation as dysfunctional HDL that could discriminate between acute coronary syndr...

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Autores principales:	Mihaela G. Carnuta, Camelia S. Stancu, Laura Toma, Gabriela M. Sanda, Loredan S. Niculescu, Mariana Deleanu, Andreea C. Popescu, Mihaela R. Popescu, Adelina Vlad, Doina R. Dimulescu, Maya Simionescu, Anca V. Sima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d4b4807917c435f8e936856e6fa55ce
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spelling	oai:doaj.org-article:5d4b4807917c435f8e936856e6fa55ce2021-12-02T12:32:33ZDysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients10.1038/s41598-017-07821-52045-2322https://doaj.org/article/5d4b4807917c435f8e936856e6fa55ce2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07821-5https://doaj.org/toc/2045-2322Abstract There is a stringent need to find means for risk stratification of coronary artery diseases (CAD) patients. We aimed at identifying alterations of plasma high-density lipoproteins (HDL) components and their validation as dysfunctional HDL that could discriminate between acute coronary syndrome (ACS) and stable angina (SA) patients. HDL2 and HDL3 were isolated from CAD patients’ plasma and healthy subjects. ApolipoproteinAI (apoAI), apoAII, apoCIII, malondialdehyde (MDA), myeloperoxidase (MPO), ceruloplasmin and paraoxonase1 (PON1) were assessed. The anti-inflammatory potential of HDL subfractions was tested by evaluating the secreted inflammatory molecules of tumor necrosis factor α-activated endothelial cells (EC) upon co-incubation with HDL2 or HDL3. We found in ACS versus SA patients: 40% increased MPO, MDA, apoCIII in HDL2 and HDL3, 35% augmented apoAII in HDL2, and in HDL3 increased ceruloplasmin, decreased apoAII (40%) and PON1 protein and activity (15% and 25%). Co-incubation of activated EC with HDL2 or HDL3 from CAD patients induced significantly increased levels of secreted inflammatory molecules, 15–20% more for ACS versus SA. In conclusion, the assessed panel of markers correlates with the reduced anti-inflammatory potential of HDL subfractions isolated from ACS and SA patients (mostly for HDL3 from ACS) and can discriminate between these two groups of CAD patients.Mihaela G. CarnutaCamelia S. StancuLaura TomaGabriela M. SandaLoredan S. NiculescuMariana DeleanuAndreea C. PopescuMihaela R. PopescuAdelina VladDoina R. DimulescuMaya SimionescuAnca V. SimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mihaela G. Carnuta Camelia S. Stancu Laura Toma Gabriela M. Sanda Loredan S. Niculescu Mariana Deleanu Andreea C. Popescu Mihaela R. Popescu Adelina Vlad Doina R. Dimulescu Maya Simionescu Anca V. Sima Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
description	Abstract There is a stringent need to find means for risk stratification of coronary artery diseases (CAD) patients. We aimed at identifying alterations of plasma high-density lipoproteins (HDL) components and their validation as dysfunctional HDL that could discriminate between acute coronary syndrome (ACS) and stable angina (SA) patients. HDL2 and HDL3 were isolated from CAD patients’ plasma and healthy subjects. ApolipoproteinAI (apoAI), apoAII, apoCIII, malondialdehyde (MDA), myeloperoxidase (MPO), ceruloplasmin and paraoxonase1 (PON1) were assessed. The anti-inflammatory potential of HDL subfractions was tested by evaluating the secreted inflammatory molecules of tumor necrosis factor α-activated endothelial cells (EC) upon co-incubation with HDL2 or HDL3. We found in ACS versus SA patients: 40% increased MPO, MDA, apoCIII in HDL2 and HDL3, 35% augmented apoAII in HDL2, and in HDL3 increased ceruloplasmin, decreased apoAII (40%) and PON1 protein and activity (15% and 25%). Co-incubation of activated EC with HDL2 or HDL3 from CAD patients induced significantly increased levels of secreted inflammatory molecules, 15–20% more for ACS versus SA. In conclusion, the assessed panel of markers correlates with the reduced anti-inflammatory potential of HDL subfractions isolated from ACS and SA patients (mostly for HDL3 from ACS) and can discriminate between these two groups of CAD patients.
format	article
author	Mihaela G. Carnuta Camelia S. Stancu Laura Toma Gabriela M. Sanda Loredan S. Niculescu Mariana Deleanu Andreea C. Popescu Mihaela R. Popescu Adelina Vlad Doina R. Dimulescu Maya Simionescu Anca V. Sima
author_facet	Mihaela G. Carnuta Camelia S. Stancu Laura Toma Gabriela M. Sanda Loredan S. Niculescu Mariana Deleanu Andreea C. Popescu Mihaela R. Popescu Adelina Vlad Doina R. Dimulescu Maya Simionescu Anca V. Sima
author_sort	Mihaela G. Carnuta
title	Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
title_short	Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
title_full	Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
title_fullStr	Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
title_full_unstemmed	Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
title_sort	dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5d4b4807917c435f8e936856e6fa55ce
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Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients

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