Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein

Dorival Martins1, Lucas Frungillo2, Maristela C Anazzetti2, Patr&iacute;cia S Melo3, Nelson Dur&aacute;n11Institute of Chemistry, Biological Chemistry Laboratory, Universidade Estadual de Campinas-UNICAMP, C.P. 6154, CE P 13083-970, Campinas, SP, Brazil; 2Institute of Biology, Cell C...

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Autores principales:	Dorival Martins, Lucas Frungillo, Maristela C Anazzetti, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5d62333de7b64a49ab13c42b8afed663
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spelling	oai:doaj.org-article:5d62333de7b64a49ab13c42b8afed6632021-12-02T02:42:31ZAntitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein1176-91141178-2013https://doaj.org/article/5d62333de7b64a49ab13c42b8afed6632010-01-01T00:00:00Zhttp://www.dovepress.com/antitumoral-activity-of-l-ascorbic-acid-poly-dl-lactide-co-glycolide-n-a3934https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Dorival Martins1, Lucas Frungillo2, Maristela C Anazzetti2, Patr&iacute;cia S Melo3, Nelson Dur&aacute;n11Institute of Chemistry, Biological Chemistry Laboratory, Universidade Estadual de Campinas-UNICAMP, C.P. 6154, CE P 13083-970, Campinas, SP, Brazil; 2Institute of Biology, Cell Cultures and Biopharmaceutical Laboratory, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brazil; 3Campinas Integrated Metropolitan Faculties-METROCAMP, Campinas, SP, BrazilAbstract: It has been demonstrated that tumoral cells have a higher uptake of ascorbic acid compared to normal cells. This differential characteristic can be used as a way to improve the specificity of antitumoral compounds if combined with polymeric drug delivery systems. The aim of this study was to prepare, characterize and evaluate the antitumoral activity of poly-D,L-(lactide-co-glycolide) 50:50 loading the antitumoral compound violacein and capped with L-ascorbic acid. Nanoparticles were prepared using the nanoprecipitation method and morphologically characterized by scanning electron microscopy (SEM). The average diameter and Zeta potential were determined by photon correlation spectroscopy method (PCS), and assays were carried out to determine the content of ascorbic acid and in vitro drug release kinetics. The antitumoral activity of this system was also evaluated against HL-60 cells by tetrazolium reduction assay. Nanoparticles with size distribution between 300&ndash;400 nm and strong negative outer surface (-40 mV) were obtained by this method. Analysis of ascorbic acid content showed that this compound was mainly localized on the external surface of nanoparticles. Violacein loading efficiency was determined as 32% &plusmn; 1% and this drug was gradually released from nanoparticles at different rates depending on the composition of the release media. In addition, this system was observed to be 2 &times; more efficient as an antitumoral compared with free violacein.Keywords: violacein, ascorbic acid, nanoparticles, PLGA Dorival MartinsLucas FrungilloMaristela C Anazzettiet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 77-85 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dorival Martins Lucas Frungillo Maristela C Anazzetti et al Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
description	Dorival Martins1, Lucas Frungillo2, Maristela C Anazzetti2, Patr&iacute;cia S Melo3, Nelson Dur&aacute;n11Institute of Chemistry, Biological Chemistry Laboratory, Universidade Estadual de Campinas-UNICAMP, C.P. 6154, CE P 13083-970, Campinas, SP, Brazil; 2Institute of Biology, Cell Cultures and Biopharmaceutical Laboratory, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brazil; 3Campinas Integrated Metropolitan Faculties-METROCAMP, Campinas, SP, BrazilAbstract: It has been demonstrated that tumoral cells have a higher uptake of ascorbic acid compared to normal cells. This differential characteristic can be used as a way to improve the specificity of antitumoral compounds if combined with polymeric drug delivery systems. The aim of this study was to prepare, characterize and evaluate the antitumoral activity of poly-D,L-(lactide-co-glycolide) 50:50 loading the antitumoral compound violacein and capped with L-ascorbic acid. Nanoparticles were prepared using the nanoprecipitation method and morphologically characterized by scanning electron microscopy (SEM). The average diameter and Zeta potential were determined by photon correlation spectroscopy method (PCS), and assays were carried out to determine the content of ascorbic acid and in vitro drug release kinetics. The antitumoral activity of this system was also evaluated against HL-60 cells by tetrazolium reduction assay. Nanoparticles with size distribution between 300&ndash;400 nm and strong negative outer surface (-40 mV) were obtained by this method. Analysis of ascorbic acid content showed that this compound was mainly localized on the external surface of nanoparticles. Violacein loading efficiency was determined as 32% &plusmn; 1% and this drug was gradually released from nanoparticles at different rates depending on the composition of the release media. In addition, this system was observed to be 2 &times; more efficient as an antitumoral compared with free violacein.Keywords: violacein, ascorbic acid, nanoparticles, PLGA
format	article
author	Dorival Martins Lucas Frungillo Maristela C Anazzetti et al
author_facet	Dorival Martins Lucas Frungillo Maristela C Anazzetti et al
author_sort	Dorival Martins
title	Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
title_short	Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
title_full	Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
title_fullStr	Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
title_full_unstemmed	Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein
title_sort	antitumoral activity of l-ascorbic acid-poly-d,l-(lactide-co-glycolide) nanoparticles containing violacein
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/5d62333de7b64a49ab13c42b8afed663
work_keys_str_mv	AT dorivalmartins antitumoralactivityoflascorbicacidpolydllactidecoglycolidenanoparticlescontainingviolacein AT lucasfrungillo antitumoralactivityoflascorbicacidpolydllactidecoglycolidenanoparticlescontainingviolacein AT maristelacanazzetti antitumoralactivityoflascorbicacidpolydllactidecoglycolidenanoparticlescontainingviolacein AT etal antitumoralactivityoflascorbicacidpolydllactidecoglycolidenanoparticlescontainingviolacein
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Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein

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