Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice

Abstract Suprachiasmatic nucleus circadian oscillatory protein (SCOP) (a.k.a. PHLPP1) regulates long-term memory consolidation in the brain. Using a mouse model of controlled cortical impact (CCI) we tested if (1) brain tissue levels of SCOP/PHLPP1 increase after a traumatic brain injury (TBI), and...

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Autores principales:	Travis C. Jackson, C. Edward Dixon, Keri Janesko-Feldman, Vincent Vagni, Shawn E. Kotermanski, Edwin K. Jackson, Patrick M. Kochanek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5d728bdcc90544d2bf5dd7ed47464977
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spelling	oai:doaj.org-article:5d728bdcc90544d2bf5dd7ed474649772021-12-02T15:08:45ZAcute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice10.1038/s41598-018-25371-22045-2322https://doaj.org/article/5d728bdcc90544d2bf5dd7ed474649772018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25371-2https://doaj.org/toc/2045-2322Abstract Suprachiasmatic nucleus circadian oscillatory protein (SCOP) (a.k.a. PHLPP1) regulates long-term memory consolidation in the brain. Using a mouse model of controlled cortical impact (CCI) we tested if (1) brain tissue levels of SCOP/PHLPP1 increase after a traumatic brain injury (TBI), and (2) if SCOP/PHLPP1 gene knockout (KO) mice have improved (or worse) neurologic outcomes. Blood chemistry (pH, pCO2, pO2, pSO2, base excess, sodium bicarbonate, and osmolarity) and arterial pressure (MAP) differed in isoflurane anesthetized WT vs. KOs at baseline and up to 1 h post-injury. CCI injury increased cortical/hippocampal SCOP/PHLPP1 levels in WTs 7d and 14d post-injury. Injured KOs had higher brain tissue levels of phosphorylated AKT (pAKT) in cortex (14d post-injury), and higher levels of phosphorylated MEK (pMEK) in hippocampus (7d and 14d post-injury) and in cortex (7d post-injury). Consistent with an important role of SCOP/PHLPP1 on memory function, injured-KOs had near normal performance on the probe trial of the Morris water maze, whereas injured-WTs were impaired. CA1/CA3 hippocampal survival was lower in KOs vs. WTs 24 h post-injury but equivalent by 7d. No difference in 21d cortical lesion volume was detected. SCOP/PHLPP1 overexpression in cultured rat cortical neurons had no effect on 24 h cell death after a mechanical stretch-injury.Travis C. JacksonC. Edward DixonKeri Janesko-FeldmanVincent VagniShawn E. KotermanskiEdwin K. JacksonPatrick M. KochanekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Travis C. Jackson C. Edward Dixon Keri Janesko-Feldman Vincent Vagni Shawn E. Kotermanski Edwin K. Jackson Patrick M. Kochanek Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice
description	Abstract Suprachiasmatic nucleus circadian oscillatory protein (SCOP) (a.k.a. PHLPP1) regulates long-term memory consolidation in the brain. Using a mouse model of controlled cortical impact (CCI) we tested if (1) brain tissue levels of SCOP/PHLPP1 increase after a traumatic brain injury (TBI), and (2) if SCOP/PHLPP1 gene knockout (KO) mice have improved (or worse) neurologic outcomes. Blood chemistry (pH, pCO2, pO2, pSO2, base excess, sodium bicarbonate, and osmolarity) and arterial pressure (MAP) differed in isoflurane anesthetized WT vs. KOs at baseline and up to 1 h post-injury. CCI injury increased cortical/hippocampal SCOP/PHLPP1 levels in WTs 7d and 14d post-injury. Injured KOs had higher brain tissue levels of phosphorylated AKT (pAKT) in cortex (14d post-injury), and higher levels of phosphorylated MEK (pMEK) in hippocampus (7d and 14d post-injury) and in cortex (7d post-injury). Consistent with an important role of SCOP/PHLPP1 on memory function, injured-KOs had near normal performance on the probe trial of the Morris water maze, whereas injured-WTs were impaired. CA1/CA3 hippocampal survival was lower in KOs vs. WTs 24 h post-injury but equivalent by 7d. No difference in 21d cortical lesion volume was detected. SCOP/PHLPP1 overexpression in cultured rat cortical neurons had no effect on 24 h cell death after a mechanical stretch-injury.
format	article
author	Travis C. Jackson C. Edward Dixon Keri Janesko-Feldman Vincent Vagni Shawn E. Kotermanski Edwin K. Jackson Patrick M. Kochanek
author_facet	Travis C. Jackson C. Edward Dixon Keri Janesko-Feldman Vincent Vagni Shawn E. Kotermanski Edwin K. Jackson Patrick M. Kochanek
author_sort	Travis C. Jackson
title	Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice
title_short	Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice
title_full	Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice
title_fullStr	Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice
title_full_unstemmed	Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice
title_sort	acute physiology and neurologic outcomes after brain injury in scop/phlpp1 ko mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5d728bdcc90544d2bf5dd7ed47464977
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Acute Physiology and Neurologic Outcomes after Brain Injury in SCOP/PHLPP1 KO Mice

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