Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

Summary: Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse...

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Autores principales:	Mai T. Dang, Fernanda Mafra, Malay Haldar
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell isolation Single Cell Cancer Sequencing RNAseq Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/5d7600e4e1c446369e89fba784c30532
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spelling	oai:doaj.org-article:5d7600e4e1c446369e89fba784c305322021-11-18T04:51:42ZIsolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis2666-166710.1016/j.xpro.2021.100957https://doaj.org/article/5d7600e4e1c446369e89fba784c305322021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006638https://doaj.org/toc/2666-1667Summary: Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis.For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).Mai T. DangFernanda MafraMalay HaldarElsevierarticleCell BiologyCell isolationSingle CellCancerSequencingRNAseqScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100957- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell isolation Single Cell Cancer Sequencing RNAseq Science (General) Q1-390
spellingShingle	Cell Biology Cell isolation Single Cell Cancer Sequencing RNAseq Science (General) Q1-390 Mai T. Dang Fernanda Mafra Malay Haldar Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
description	Summary: Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis.For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).
format	article
author	Mai T. Dang Fernanda Mafra Malay Haldar
author_facet	Mai T. Dang Fernanda Mafra Malay Haldar
author_sort	Mai T. Dang
title	Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_short	Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_full	Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_fullStr	Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_full_unstemmed	Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis
title_sort	isolation of myeloid cells from mouse brain tumors for single-cell rna-seq analysis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5d7600e4e1c446369e89fba784c30532
work_keys_str_mv	AT maitdang isolationofmyeloidcellsfrommousebraintumorsforsinglecellrnaseqanalysis AT fernandamafra isolationofmyeloidcellsfrommousebraintumorsforsinglecellrnaseqanalysis AT malayhaldar isolationofmyeloidcellsfrommousebraintumorsforsinglecellrnaseqanalysis
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Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

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