<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation

ABSTRACT Bacterial cells need to coordinate the cell cycle with biomass growth to maintain cell size homeostasis. For fast-growing bacterial species like Escherichia coli and Bacillus subtilis, it is well-known that cell size exhibits a strong dependence on the growth rate under different nutrient c...

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Autores principales:	Xiongfeng Dai, Zichu Shen, Yiheng Wang, Manlu Zhu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Sinorhizobium meliloti cell cycle cell size growth rate Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5d7635700efa4cd4a0d3b2907bb4fe0d
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spelling	oai:doaj.org-article:5d7635700efa4cd4a0d3b2907bb4fe0d2021-11-15T15:22:21Z<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation10.1128/mSphere.00567-182379-5042https://doaj.org/article/5d7635700efa4cd4a0d3b2907bb4fe0d2018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00567-18https://doaj.org/toc/2379-5042ABSTRACT Bacterial cells need to coordinate the cell cycle with biomass growth to maintain cell size homeostasis. For fast-growing bacterial species like Escherichia coli and Bacillus subtilis, it is well-known that cell size exhibits a strong dependence on the growth rate under different nutrient conditions (known as the nutrient growth law). However, cell size changes little with slow growth (doubling time of >90 min) for E. coli, posing the interesting question of whether slow-growing bacteria species also observe the nutrient growth law. Here, we quantitatively characterize the cell size and cell cycle parameter of a slow-growing bacterium, Sinorhizobium meliloti, at different nutrient conditions. We find that S. meliloti exhibits a threefold change in its cell size when its doubling time varies from 2 h to 6 h. Moreover, the progression rate of its cell cycle is much longer than that of E. coli, suggesting a delicate coordination between the cell cycle progression rate and the biomass growth rate. Our study shows that the nutrient growth law holds robustly regardless of the growth capacity of the bacterial species, generalizing its applicability among the bacterial kingdom. IMPORTANCE The dependence of cell size on growth rate is a fundamental principle in the field of bacterial cell size regulation. Previous studies of cell size regulation mainly focus on fast-growing bacterial species such as Escherichia coli and Bacillus subtilis. We find here that Sinorhizobium meliloti, a slow-growing bacterium, exhibits a remarkable growth rate-dependent cell size pattern under nutrient limitation, generalizing the applicability of the empirical nutrient growth law of cell size. Moreover, S. meliloti exhibits a much slower speed of cell cycle progression than E. coli does, suggesting a delicate coordination between the cell cycle progression rate and the biomass growth rate.Xiongfeng DaiZichu ShenYiheng WangManlu ZhuAmerican Society for MicrobiologyarticleSinorhizobium meliloticell cyclecell sizegrowth rateMicrobiologyQR1-502ENmSphere, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sinorhizobium meliloti cell cycle cell size growth rate Microbiology QR1-502
spellingShingle	Sinorhizobium meliloti cell cycle cell size growth rate Microbiology QR1-502 Xiongfeng Dai Zichu Shen Yiheng Wang Manlu Zhu <named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
description	ABSTRACT Bacterial cells need to coordinate the cell cycle with biomass growth to maintain cell size homeostasis. For fast-growing bacterial species like Escherichia coli and Bacillus subtilis, it is well-known that cell size exhibits a strong dependence on the growth rate under different nutrient conditions (known as the nutrient growth law). However, cell size changes little with slow growth (doubling time of >90 min) for E. coli, posing the interesting question of whether slow-growing bacteria species also observe the nutrient growth law. Here, we quantitatively characterize the cell size and cell cycle parameter of a slow-growing bacterium, Sinorhizobium meliloti, at different nutrient conditions. We find that S. meliloti exhibits a threefold change in its cell size when its doubling time varies from 2 h to 6 h. Moreover, the progression rate of its cell cycle is much longer than that of E. coli, suggesting a delicate coordination between the cell cycle progression rate and the biomass growth rate. Our study shows that the nutrient growth law holds robustly regardless of the growth capacity of the bacterial species, generalizing its applicability among the bacterial kingdom. IMPORTANCE The dependence of cell size on growth rate is a fundamental principle in the field of bacterial cell size regulation. Previous studies of cell size regulation mainly focus on fast-growing bacterial species such as Escherichia coli and Bacillus subtilis. We find here that Sinorhizobium meliloti, a slow-growing bacterium, exhibits a remarkable growth rate-dependent cell size pattern under nutrient limitation, generalizing the applicability of the empirical nutrient growth law of cell size. Moreover, S. meliloti exhibits a much slower speed of cell cycle progression than E. coli does, suggesting a delicate coordination between the cell cycle progression rate and the biomass growth rate.
format	article
author	Xiongfeng Dai Zichu Shen Yiheng Wang Manlu Zhu
author_facet	Xiongfeng Dai Zichu Shen Yiheng Wang Manlu Zhu
author_sort	Xiongfeng Dai
title	<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
title_short	<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
title_full	<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
title_fullStr	<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
title_full_unstemmed	<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
title_sort	<named-content content-type="genus-species">sinorhizobium meliloti</named-content>, a slow-growing bacterium, exhibits growth rate dependence of cell size under nutrient limitation
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/5d7635700efa4cd4a0d3b2907bb4fe0d
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<named-content content-type="genus-species">Sinorhizobium meliloti</named-content>, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation

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